Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_LN832404 | 464,304 | A→G | 71.0% | V181V (GTT→GTC) | queC ← | 7‑cyano‑7‑deazaguanine synthase QueC |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_LN832404 | 464,304 | 0 | A | G | 71.0% | 243.3 / 133.3 | 162 | V181V (GTT→GTC) | queC | 7‑cyano‑7‑deazaguanine synthase QueC |
Reads supporting (aligned to +/- strand): ref base A (22/25); new base G (57/58); total (79/83) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.63e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 |
Only 100 of 178 total aligned reads displayed.
ACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTT > NZ_LN832404/464013‑464593 | agggttgttggtATTGATCTAAAAAAGCAGAAACAAGTGGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTGGTGGTGCCGTATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCAGCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGAc < 2:256476/290‑1 (MQ=255) aCGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGTAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAAc < 2:44828/294‑1 (MQ=255) cGTTTGTTTGTATTGAGCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACt < 1:6768/294‑1 (MQ=255) gtttgtttGTATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTa < 1:407932/294‑1 (MQ=255) gtgtgttgttATTGATCTTAAAGTCTAGAAGGCATTAG‑TCTAGTATGGGGAATTTCGCGAAAAGCTGTTGCAGATGGTGAGGTAAACGTTTTGTGTTGCCGGATTCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTa < 2:1026867/285‑1 (MQ=255) tttgtATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATcc < 1:410310/294‑1 (MQ=255) tttgtATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATcc > 1:1064232/1‑294 (MQ=255) tgtATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATACTGATTCAAACCGTTGGCGCGTAAATTACCTTCCGCACAATGAACCAACCCGTCCCCCATAACGCCGTATTAACCGGTCAA‑CGTTTCGTT‑TCGGCCTAAATcaca > 2:760664/1‑291 (MQ=255) gtATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTGTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGt < 1:680884/294‑1 (MQ=255) tCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGt < 1:478407/294‑1 (MQ=255) ttAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCCTCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCAGTTATAGCCGGTAAA‑CGTTTCGTT‑ACGCAATAAATCCAATTTGACGtaa > 2:322774/1‑294 (MQ=255) aaaaccacgAAATCATTAGCGTG‑GGAAGGGGAATGACGGGAAAAGCTGGAGCAGACGGTTAAGTAACCGTTTTGTGGTGCCGGAGGCCCAAGCCCCATCCGGCGACACCCGGAATAAGTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGtaata < 2:605064/286‑1 (MQ=255) aCGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCATATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGGAATAATc < 1:628952/294‑1 (MQ=255) cGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCt < 1:40031/294‑1 (MQ=255) gAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCa < 1:541152/294‑1 (MQ=255) aGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGAACGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAgcg > 2:449448/1‑294 (MQ=255) ttAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCa < 1:37368/294‑1 (MQ=255) ttAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCa > 1:955586/1‑294 (MQ=255) aGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAAACCAGTTTGCCGTAATAATCCGCCAGCCCCCAgg > 2:842293/1‑294 (MQ=255) tct‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACCTGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑CCGGACTAACTACAGTTTGCCGTAATAATCTGCCCGCGCACAGGttt > 2:368427/1‑294 (MQ=255) t‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTcc > 1:251089/1‑294 (MQ=255) tGCGCGAAAAGCTGGACAAGATGTTGAAGTAAACGTTTTTTGGTGCCGGATGCTCAAGCCGCATCCGGCGACCCCCGGAATAATTCCCTCAACCCGGTTTGCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATc < 2:666456/294‑1 (MQ=255) gAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑t > 1:503217/1‑294 (MQ=255) gAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑t > 2:65327/1‑294 (MQ=255) aaaGGTGGGGAAGAGGGTTAAGTAAACGTTTGGTGGTGCCGGATGCTCAAGCCGCATCCGGAGAAACCCGGACTAATTACCTCACCCCGGTTTTCTGCGTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCa < 2:511411/294‑1 (MQ=255) ggAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAgcggcg < 1:132531/294‑1 (MQ=255) tGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCaaaa > 1:443273/1‑294 (MQ=255) gTTAAGGAAACGGTTTGTGGTGCAGGATGCTCAATCCGAATCCGGCGACACCCGGAAGAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGAACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACg < 2:973854/294‑1 (MQ=255) aGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ATGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAata > 1:344138/1‑294 (MQ=255) aaCGTGTTGTGGTGCCGGAGGCTCAAGCCGCATCCGGCGACAACCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCtt < 1:556895/294‑1 (MQ=255) aCGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCttt > 1:217598/1‑294 (MQ=255) aCGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCCTTTCAAAAccacatacctt > 2:46155/1‑284 (MQ=255) cGTTTTGTGGTGCCGGATGCGCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTc < 1:91704/294‑1 (MQ=255) gTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCg < 1:1041040/294‑1 (MQ=255) tgtgGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGGGGCGTTTCAAAACGAATATCTTTCGCCAt < 1:796242/294‑1 (MQ=255) tgtgGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCAt < 1:486661/294‑1 (MQ=255) tgtgGTGCCGGATGCCCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACAGTCGCCTTTAAAGCCGTTCTACCAGGTCAA‑AGTTTCGTT‑ACGGACTAAATCCCACTTGCCGTAATAATCTGCCAGCGCCACGGGTCCCGCTTTTTCACTCCACA‑TCCACCGCCTGTAAAAACCAACCTCTTTCGcact > 2:768901/1‑291 (MQ=255) tGCTCAAGCCGCATCCGGCGACACCCGTAATAATTACCTCATCCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGAc < 1:1003166/294‑1 (MQ=255) tGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATTAATCCACACTCA‑CGCAGTTCCAAAAAGGATAACTTTCCCCTTTCCCAAACCGAc > 2:305926/1‑294 (MQ=255) ccctgtCCGGCGCCCCCCGCATTAATCACCTCAACCCTGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGt < 2:143623/289‑1 (MQ=255) cGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGtt < 1:1000721/294‑1 (MQ=255) ggCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCt > 1:295675/1‑294 (MQ=255) gCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTCAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCCATCCCCA‑TCAGCGGCGTTTCAAAACCAATATTCTTCGCACTTCCCAAAC‑CTCAGCACTGGTT‑TAGTGCtt > 2:1051541/1‑294 (MQ=255) gACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTc > 1:279442/1‑294 (MQ=255) gACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGCAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATACATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCACAAACTGACGGCA‑TGGTT‑TAGTGCttta > 1:804366/1‑293 (MQ=255) cacCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACCAATATCTTTCGCCATTCCCAAACTGACGGCC‑TGGTT‑TAGTGCTTTcac > 1:8619/1‑294 (MQ=255) cGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCCGTTTGCCGTAATAATCTGCCAGCCACCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGCATATCTTTCGCCATCCCCAAACTGCAGGCA‑TGGTT‑TAGTGCTTTcaacacc > 2:779963/1‑289 (MQ=255) gcataatTACCTCAACCCGGTTTGCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTc < 2:250492/292‑1 (MQ=255) ataatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCAt < 1:903458/294‑1 (MQ=255) taatTACCTCACCCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTGTATCGGCCAGATAATGATTCAAACCGGTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATc < 2:794425/294‑1 (MQ=255) aatTACCTCAACCCGTGTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATcg < 1:965254/294‑1 (MQ=255) aatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATcg < 1:65327/294‑1 (MQ=255) aatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCC‑TGGTT‑TAGTGCTTTCACAAA‑CTCATcg > 1:205480/1‑294 (MQ=255) aatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCC‑TGGTT‑TAGTGCTTTCACAAA‑CTCATcg > 1:753975/1‑294 (MQ=255) aatTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGAAACCGTCGCCTTTAAAGCCGTTATAGCCGGTCCA‑CGTTTCGTT‑ACGGACTAAATCCAGATTGACGTAATCATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACC‑TCAGCGGCGTTTCAAACCGAATCTCTTTCTCCAAGCCCCAAATTACGGCC‑TAGTTCTAGT‑CTTTTACAAACCTCATc > 2:813662/1‑294 (MQ=255) atTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCCGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTAAAAACGAATCTCTTTCGCCATGCCCCAACTGACCGCC‑TGGTT‑TCGTGCTTTCACCAA‑CCCATccc > 2:1014345/1‑292 (MQ=255) atTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATcgc < 2:1064232/294‑1 (MQ=255) cGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGt > 1:177878/1‑294 (MQ=255) ttttCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGc > 1:992895/1‑294 (MQ=255) tgttCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGAGTCAAACCGTTGGCGCGTAAATTACATGCCGAACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGc < 2:587295/292‑1 (MQ=255) tGCGTCATCGCGTCCATAACCGGCGTGTTTTCGGCCAAAAAATGATTCAAACCGTGGGCGCGTAAATTAAATTCCGAACAAGGACCGCAACCGTCGCCTTTAAGGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGgag < 2:818568/294‑1 (MQ=255) aTCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAACACGAATATCTTTCGCCATGCCCAAACTGACCGCA‑TGGGT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGATCTCCGGAGAAATcc > 2:284807/1‑294 (MQ=255) aTCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATcc < 1:901791/294‑1 (MQ=255) ccATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGc < 1:486928/294‑1 (MQ=255) tCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTCTCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGccgc > 1:262198/1‑291 (MQ=255) tCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGa > 1:891399/1‑294 (MQ=255) aaCCGTGGGTTTATCGGCCAGATAAGTATTAAAACCGTTGGCGCGTAAATTAAATGCCGCAAAATGACCGCAACCGTGGCCTTTAAATCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGAc < 2:78997/293‑1 (MQ=255) aCCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACg > 1:524428/1‑294 (MQ=255) aCCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACg < 1:388242/294‑1 (MQ=255) gTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCCTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGCAGAAATCCGTTTCGAAGCCGCCa > 2:880275/1‑294 (MQ=255) tatagaccAGATAATGATTCAAACCGTTGGCGCGTAAATTACAGGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTa < 1:140600/288‑1 (MQ=255) tCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACg < 1:763007/294‑1 (MQ=255) ggCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTCATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACCAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCCGACGCACGTAATTACCGc > 1:595241/1‑294 (MQ=255) gCCAGATAAGGAGTGAAACCGTTGGCTCGTAAAGTACAGACCGAAAAATGACCGCAACCGTCGCCGTTAAAGCCGTGATAGCAGGTCAA‑CGTGTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCt < 2:869391/294‑1 (MQ=255) aTAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAC‑CTCATCGCGGCAATCCGGGTCGCCGGAGAAATCCGTTTCCCAGACGCCACTCATTACGCCTTCTGc > 2:422907/1‑294 (MQ=255) aTAAGGATTCAATCCGGTGGAGCTTAAATTACAGGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGGTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGc < 2:742203/294‑1 (MQ=255) tAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCCGTAATTACGGCTTCTGCt > 1:374325/1‑294 (MQ=255) ttCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACCGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAAACCGGGTAGCCGGACAAATACGTTTCGCAGAAGCCCGTAATTTCGGCATCTGCTTTTAcc > 2:695669/1‑294 (MQ=255) aCCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATa < 1:63574/294‑1 (MQ=255) aaaTTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTgcc < 1:842293/294‑1 (MQ=255) aTGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑aaaca > 1:83785/1‑291 (MQ=255) gtaCCCCAACCGTCCGCTTTAATACCGTTAGAGAATGTCAA‑CGGTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTGTCCGCTTTATCAATCCACA‑GCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACaaa < 2:64099/292‑1 (MQ=255) aCCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAat > 1:876939/1‑294 (MQ=255) cGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCTCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATCCGCATATATTGCCGCCAGCGCCCGG‑acccacattt > 2:93947/1‑285 (MQ=255) gCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATAtt < 1:21196/294‑1 (MQ=255) cAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCTCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCCGCCGCCAGTAATTACGGCTTCCGCTTTTACCTGTTACGCATATATTTCCGCCAGCGTCCGGCaacaattctta > 2:648565/1‑288 (MQ=255) ccccGTCGCCTTTAAAGCCG‑TATCACCGGGCAACCTTTTCCTTCACG‑CCTAAATCCCGTTGCCCTGAATAATCTGCCAGCGCCCAGGTTTCCCCTTTACCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTAc < 2:524428/292‑1 (MQ=255) aCCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATCTCTTGCCTCCTTCGTCAGG‑AACAAAATATTACg < 1:936078/294‑1 (MQ=255) cggcGCCTTGAACGCCGTTATAGCAGGTCAA‑CGGTTCGTT‑ACGGACTAAATCCAGGTTGCCGTAATAATCTGCACGCGCCCAGGTTTCCGCTGTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGcc < 2:397726/291‑1 (MQ=255) tCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCt > 1:126909/1‑294 (MQ=255) cGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGAATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTg > 2:647743/1‑294 (MQ=255) cGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTAACTGATTCGCATATATTTCAGCCAGCCTCAGG‑GACACAATATTAAGCCCCGGGACCAACGttt > 2:543789/1‑292 (MQ=255) tataGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑ACCAACATATTACGACCTGGGACAAACGTCTTCg > 1:28616/1‑294 (MQ=255) tataGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATGTTACGCCCTGGGACAAACGTATTCg < 1:647740/294‑1 (MQ=255) tataGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACGTATTCg > 2:959749/1‑294 (MQ=255) ataGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACGTATTCgg > 1:298312/1‑294 (MQ=255) taGCAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGAGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACTTATTAggg > 1:84134/1‑294 (MQ=255) cAGGTCAA‑CGTTTCGTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCCTCAGG‑CACAAAATATTAAGCCCTGGGACCAACGTATACGGGaaa > 2:158531/1‑292 (MQ=255) tCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCAt < 1:46155/294‑1 (MQ=255) gTT‑ACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTTCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACGTAATCCGGAACCCCTGGGCTTccag > 2:226757/1‑291 (MQ=255) | ACGTTTGTTTGTATTGATCTTAAAACGCAGAAAGCATTAGCTCT‑GGAAGGGGAATTGCGCGAAAAGCTGGAGCAGATGGTTAAGTAAACGTTTTGTGGTGCCGGATGCTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAA‑CGTTTCGTT‑ACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACA‑TCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCA‑TGGTT‑TAGTGCTTTCACAAA‑CTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATTGCCGCCAGCGTCAGG‑AACAAAATATTACGCCCTGGGACAAACGTATTCGGGATACCATCGGCTTCAGGTT > NZ_LN832404/464013‑464593 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |