Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 3,940,810 C→T 100% noncoding (604/2886 nt) EK12AG100_RS19545 → 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324043,940,8100CT100.0% 33.2 / NA 20noncoding (604/2886 nt)EK12AG100_RS1954523S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACT  >  NZ_LN832404/3940520‑3940861
                                                                                                                                                                                                                                                                                                   |                                                   
cgggcaaGTCGCGCGATACAAGGGTACAGCCCCGTACACAAAAATGCACATATTTTGAGCTCGATGCGTAGGGCGGGACACGTTGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGt                                                  <  1:266325/289‑1 (MQ=255)
    aaaGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTGGTAGCa                                              >  1:601312/1‑294 (MQ=17)
     aaggggaagggtACAGTGTGACAGCCCCGGAAACAAAAATGCAAATAGTGGGAGCTCGTGGAGTAGGGCGGGAGACGTGGTAGCCTGGCTGAAGAGGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGAGAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACCGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCaa                                             <  2:646055/283‑1 (MQ=11)
        aggatcgaagtggggTGAAAGGCCCGGAAACAAAAAGGACCATATGGTTAGCGCGAAGAGTAGGGCGGGACAAGTGGTAACCAGGCGGAATATGGGGAGACCATCCACCAAGGGTAAATACTCCTGACTGACCGATAGTGAACCGGTAACGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGt                                          <  2:498653/282‑1 (MQ=11)
            gcgATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCTTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTatc                                      >  1:36760/1‑292 (MQ=17)
              gATACAGTGTAACAGCCACGGAAACAAAAATGACAATATTGTGGGCTAGATGCTTAGTGCGGGACACGTGGTATCCGGTCTGAATATTGGGGGACCAACCTCCAAGGCAAAAGACTCCTGACTGACCGATAGTGAAGCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCg                                    <  2:481931/294‑1 (MQ=11)
              gATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCGTAGGCGTGTGACTGCGTACATTTGTTATAATGGGTCAGAGACTTAGA‑TGTTGTAGCAAGGTTAAcgg                                    >  2:340377/1‑292 (MQ=255)
                 aCAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCACCAAGGCTAAATACTCCTGCCTGACCGATAGTGACCCAGTACCGTGAGGGAAAGGCGAAAAGCACCCCGGCGCGGGGAGTGAAAAAGAACCTGAAACCGTGAACGTACAACCAGAGGGAGCAAGATTAGGCGTGTGAGTGCGTCCCCTTAGTAAAATGGGCCGGCGAC‑TATACTTTTGTAGAAAGGTTAACCGaag                                 >  2:161243/1‑293 (MQ=11)
                    gggTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACAGTGAGGGAAAGGCGAAACGAACCCCGGCGAGGGGAGTGAAAACGAACCTGAAACCGTGTACGTACAAGCAGAGGGAGAACGCCTAGGCGTGTGACTGCGCACCTTTTGTATAATGGGGCAGCGACTTCTA‑GTTTGTAGCAAGGTGAACCGAATAgg                              >  2:212470/1‑294 (MQ=11)
                      gTGACAGCCACGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAgggg                            <  1:554878/294‑1 (MQ=17)
                         aCAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGc                         >  1:164825/1‑294 (MQ=17)
                           atgcccGTACACAAACATGCACATATTGTGGGCTCGATGAGTACGGCGGGACACGAGGTATCCCGGCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCg                       <  2:105963/291‑1 (MQ=255)
                               ccGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGTCACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGCTAGTGAACCCGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGCGAAAAAGAACCTGAAACCGTCTACGTACCAGCAGTGGGACCACGCTTAGGCGTGTGCCTGAGTACTTTTTGTATACTGTGTCAGCGACTTAGA‑TTTTGTAGCCTGGTTACCCGAATGGGGGAGCCGACgg                   >  2:556947/1‑294 (MQ=11)
                               ccGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTGCCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAGCCTGAAACCGTGTACGTACAAGCAGTGGGAGGACGCTTAGGCGTGTGACTGCGTACCTTTTGTATACTGGGTGAGCGACTTTGC‑TGTTGTAGCGAGGTTAACCGACTAGGGCAACCGAggg                   >  2:172807/1‑294 (MQ=11)
                                 gTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAACAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCACGGTTAACCGACTAGGGGAGCCGAAgggc                 >  1:707108/1‑293 (MQ=17)
                                  tACACAAAAATGCACATAGTGTGAGCTAGATGAGGAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCAGCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGaa                <  1:188125/294‑1 (MQ=9)
                                  tACACAAAAAGGCACATATGGATGGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAAATGGGGGGACCAACCGCCAAGGCTAAATCCTCCTGACTGACCGATAGTGAGCCAGTACCGGGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGaa                <  2:275478/294‑1 (MQ=11)
                                     acaAAAATGCACATATTGTGAGCTCGATTAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACTGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAcc             <  1:252064/294‑1 (MQ=17)
                                       aaaaaTGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGCGCCGAAGGGAAACCGa           >  2:66502/1‑294 (MQ=17)
                                         aaaTGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCCGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGCGCAGAAGGGAAACCGCGt         >  2:753991/1‑294 (MQ=12)
                                          aaTGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCCGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAAAGGGTCAGCGCCTGATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGGGCCGAAGGGAAACCggttc        >  2:691087/1‑290 (MQ=12)
                                             gCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCGTTCTGAAGATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATATTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGAAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTa     <  1:499643/294‑1 (MQ=12)
                                              cacaTATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCCGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTaa    >  2:893352/1‑294 (MQ=17)
                                               acaTATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGAGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATCGGGGAGCCGAAGGGAAAACGCGTCTTAAc   >  1:738061/1‑294 (MQ=12)
                                                caTATTGGTAGCGCGATGTGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGTGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACt  <  1:553551/294‑1 (MQ=9)
                                                aatatGGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTTTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACt  <  1:769068/293‑1 (MQ=11)
                                                                                                                                                                                                                                                                                                   |                                                   
CTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATA‑TTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACT  >  NZ_LN832404/3940520‑3940861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑