Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C93.1% 451.9 / 24.1 164intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (4/7);  new base C (66/83);  total (73/91)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
*NC_0009134,296,3802.G93.2% 463.4 / 23.0 164intergenic (+586/+56)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base . (4/7);  new base G (69/83);  total (73/91)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 164 total aligned reads displayed.

GCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑C‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTT  >  NC_000913/4296193‑4296617
                                                                                                                                                                                              ||                                                                                                                                                                                                                                                   
gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGAAGC‑TTGCCGCCTCTTATCAGGCCTATCTTAAcc                                                                                                                                                                                   >  1:1172538/1‑251 (MQ=2)
     ttctgaggaccaCGCCAGGCAGCGGAATAGCCTGAGTTAGCTGGAGTTTGTGGGTCGGTTAAGGCGTTTATGCCGCATCCGCCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGGCAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                                                                                                                              <  1:1910638/240‑1 (MQ=11)
     ttatacggCCTACCCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                                                                                                                              <  1:886023/245‑1 (MQ=255)
     ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                                                                                                                              <  2:420435/251‑1 (MQ=17)
     ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                                                                                                                              <  2:148164/251‑1 (MQ=17)
     ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                                                                                                                                              <  2:1255538/251‑1 (MQ=17)
         cAGGCCTATGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCCGGCCTATTTTACCCGTTGGTTac                                                                                                                                                                          >  2:1083879/1‑250 (MQ=9)
               tacaccacccAGCACAATAGCCTGATTTCCCGTTATTTTGGATGTCGGATAAGGCGTTTATACCGCATCCGACGTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCGTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑Tc                                                                                                                                                                   <  2:983339/242‑1 (MQ=11)
                accccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑CGTC‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCa                                                                                                                                                                  <  2:679072/248‑1 (MQ=255)
                accccACACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCa                                                                                                                                                                  <  2:1031557/248‑1 (MQ=255)
                aCGCCAGACAGCGCAATAGCCTGATTTAGGGTGATTTTGTAGGTCGGTTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCa                                                                                                                                                                  <  1:1362633/251‑1 (MQ=255)
                aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt                                                                                                                                                                             <  2:163391/241‑1 (MQ=17)
                aCGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCa                                                                                                                                                                  <  2:1321344/251‑1 (MQ=255)
                  gCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCaaa                                                                                                                                                                <  2:1172550/251‑1 (MQ=255)
                    cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTTGGTCGGACAAGGCGTT‑T‑A‑‑CGTC‑GC‑ATCCGACATCAAGGCCCGATGCGACGC‑TTGCCGCGTTTTACCAGGCCTATCTTAACCGGTGGTTAATTTT‑TCaaaac                                                                                                                                                              >  1:679065/1‑249 (MQ=11)
                         aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATAAATGCCTGATGCTACGC‑TTGCCGCGTCTTATCAGGCCTATCTTACCGCTTCGTTAATTTT‑TCAACCTCTTca                                                                                                                                                         >  1:1280514/1‑251 (MQ=11)
                          gcgcgtaaGCCTGATTTAACGTAATTTTGTAGTTCGGATAAGGCGGTTAAGCCGCATCCGTCATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  1:1200149/244‑1 (MQ=14)
                          gcgcTATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  1:1805022/251‑1 (MQ=255)
                          gcgcGATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTGTATGCCGCATCCGACATAAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  2:279737/251‑1 (MQ=255)
                          gcgcAATAGCCTGTTTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCTCTTCCCACATCAACGCCTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  2:950682/251‑1 (MQ=14)
                          gcgcAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAGGCGACGCTTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTGTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  2:613898/251‑1 (MQ=255)
                          gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAGGCTTAATGCGTCTTATCAGGCCGAAGTCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGAGCAGGCGTT‑T‑ACGC‑‑C‑GC‑ATACGACCTCAATCCCGGCTGCTACGC‑TTCCCGAATCTTACAAGGCCTATCTTACCCGTGGGTCAATTTT‑TCAAACACTTcac                                                                                                                                                        >  1:139063/1‑251 (MQ=11)
                          gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  2:1874792/251‑1 (MQ=255)
                          gcccaatagctgtatgtggCGTGATTTTGTAGGTCGGATAAGGCATTTACGCCGCATCCGGCATCAACGCCTGGTGCGACGCTTACCGCGTCTTATCAGGCCTACGCCATACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  1:652219/233‑1 (MQ=14)
                              aaTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATCAGGCGTT‑T‑ACGC‑‑C‑GC‑ATACGACACCAATGCCTGATGCGACGC‑TTGCCTCGCCTTACCAGCCCTATCTTAACCGTGGGTTAATTTT‑TCAAACTCTTCTCAccc                                                                                                                                                    >  1:97283/1‑251 (MQ=14)
                                   ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGCATTAGCGTGATTTTGTCGGTCGGATCAGGCGTT‑T‑ACGC‑‑C‑GC‑ATTCGACCTCAATCACTGATTCGACGC‑TTTCCGCGTCTTAACAAGCCTCTCTTACCCGTTGGTAAATTTT‑TCACACTCTTCACACGCGGTAt                                                                                                                                               >  1:100420/1‑251 (MQ=14)
                                   ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTAt                                                                                                                                               >  2:1129744/1‑251 (MQ=255)
                                    cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATc                                                                                                                                              <  2:866626/251‑1 (MQ=255)
                                        tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCGGCGCAATAGCCTGCATTAGCGTGATTTTGTAGGTTGGATAATGCGTT‑A‑A‑‑CG‑C‑CCAAGCCGACATCCAGGCCTGACGCG‑CGCTTTGCCCACTCTTAACAGGCTTATCTTAACCGTCGGTTAATTTT‑CCAAACTCTTTACAACCGCTATCCAAc                                                                                                                                          >  1:1775284/1‑251 (MQ=14)
                                        tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGTCTGATGCGACGC‑TTGCAGCGTCTTATCAGGCCTATCTTAACCGTTGGTAAATTTT‑TCACACTGTTCACACCTGGTATCAAAc                                                                                                                                          >  2:861147/1‑251 (MQ=14)
                                        tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATCCCTGATGCGACGC‑TTGCCCTGTCTTATCAGCCCTATCTTCATCGTTGGTTAATTTT‑TCACACCCTTCACACCCGGTATCAAAc                                                                                                                                          >  1:574646/1‑251 (MQ=14)
                                        tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTAGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCTTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTT‑TCAAACCCTTCACACCCGGTATCAAAc                                                                                                                                          >  2:15332/1‑251 (MQ=255)
                                         ttggcgggttttcGTATGTCGGATAACGCGTTTTTGCCGCTTCCGACTCCACCGCTTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAAcc                                                                                                                                         <  1:377147/242‑1 (MQ=17)
                                              tcagatgtgtataaggaacagggCGTTTGTGCCGCATCCGACATCAACGGCTGATGCGACGCTTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTcc                                                                                                                                    <  1:1208432/230‑1 (MQ=17)
                                              gTGATTTTGTATGTCGGATAAGGCGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTcc                                                                                                                                    <  2:1730400/251‑1 (MQ=255)
                                              gTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTcc                                                                                                                                    <  2:507844/251‑1 (MQ=255)
                                                  ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTATGCCAGAAAGCGCAATAGCCTGATTTAGTGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCCGATGCGACGC‑TTGGCGCGGCGTATCAGGTCTATCTTACCAGTTGGTTAATTTT‑TCAAAATCTTCCAAACCGGTTGCAAAcccatacaaac                                                                                                                                >  2:1253605/1‑243 (MQ=11)
                                                   tttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                                                                                               <  2:527720/251‑1 (MQ=255)
                                                    ttGTAGGTCGGAAAAGGCGTTTAGGCCCCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAGACTCTTCACACCCGGTATCAAACCCTTCCATACAg                                                                                                                              <  1:310986/251‑1 (MQ=255)
                                                     tGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACAcc                                                                                                                                                     >  2:1829711/1‑227 (MQ=255)
                                                         ggTCGGATAAGGCGTTTATGCCGCAACCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATACCCTGATTTAGCGTGATTTTGTACGCCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATCCCTGAGGAGAGCC‑TTGCCGCCTCTTCTCAGGCCTATCTTAACCGTGGGTTAATTTT‑CCAAACTCTTCCCCCCCGGTAACAAATCTTTCCATACACCTccg                                                                                                                         >  1:1028301/1‑249 (MQ=14)
                                                          gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑TC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCACACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgg                                                                                                                        >  1:1219159/1‑251 (MQ=255)
                                                          gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTACCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTCGTAGGTCGGATAAGTCGCT‑T‑ACGC‑‑C‑GC‑ATCAGACATCAATGCCTGATGCGAAGC‑TTGCCGTGTCTTACCAGGCCTATCTTAACCGTTGGATAATTTTGTCACA‑TCTTCACAATCGGCATCAAACCCTTACATACCGCTCCgg                                                                                                                        >  1:1702861/1‑251 (MQ=14)
                                                              gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTG‑TCAAACTCTTCACACCCGGTATAAAACCCTTCCATCCAGCTCAGGtttc                                                                                                                    >  2:86997/1‑249 (MQ=255)
                                                                   ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTcac                                                                                                                                                        <  1:1620630/210‑1 (MQ=255)
                                                                         tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc                                                                                                         >  1:1448685/1‑251 (MQ=255)
                                                                         tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCCGATTTAGTGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgc                                                                                                         >  2:488095/1‑251 (MQ=255)
                                                                            gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGcc                                                                                                      >  2:1163981/1‑251 (MQ=255)
                                                                             ccacATCCGCCATCACCGCCTAATGCTACGCTTAACGCGTCTTATCAGGGCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCGTACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                     <  2:1002169/248‑1 (MQ=18)
                                                                             ccGCCTCCGACATCAACGCCTGATGGGACGCTTAACGCGTTTTCTCAGGCCTATGCCAGACAGCGGAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                     <  1:1074615/251‑1 (MQ=18)
                                                                                 aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTg                                                                                                 >  2:245290/1‑251 (MQ=255)
                                                                                      ccagcaacccctgagcctccGCTTAGCGCCGCTTACCAGACCGACGCATGAAAGCGAAAGTGCCTAATTTAGCGTTATTTTGTAGGTACGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATTCCTGAGTCGACGC‑TGTCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAGCTCTTCACCCCCGGTATCAAACCCTTCCATACAGCTCAGGTGCAACCAGTGCAGCGCCTTTTGCTTGt                                                                                            <  1:850931/232‑1 (MQ=16)
                                                                                      acagtcaggCCTAATGCGACGCTTAACGCGTCTTATCAGACCGACGCCAGACTGCGCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGt                                                                                            <  2:53997/243‑1 (MQ=21)
                                                                                      aCATGAACGCCCGATGCAAAGCTTAACGCGTCTTTTCAGGGTTCCGCACGACGACTCAGTAGCCTGATTTAGCGTGATTTGTTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑AACCGACATCAATGCCTGCTGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGt                                                                                            <  1:1864275/251‑1 (MQ=21)
                                                                                      aCATCAACGCCTGGTGGCACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGt                                                                                            <  2:1280529/251‑1 (MQ=255)
                                                                                           aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                >  2:1620667/1‑210 (MQ=255)
                                                                                           aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGc                                                                                                            >  2:1208446/1‑230 (MQ=255)
                                                                                              gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ATGC‑‑C‑GC‑ATCCGACATCAACGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTGTCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCg                                                                                    <  1:152316/251‑1 (MQ=255)
                                                                                              gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCg                                                                                    <  1:245289/251‑1 (MQ=255)
                                                                                              gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCg                                                                                    <  1:1621852/251‑1 (MQ=255)
                                                                                               ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ATGA‑‑C‑GC‑ATCCGAAATCAATGCCTGATGCGAAGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCTGGTATCAAACCCTTCCATACAGCTCAGGTTCAAACAGTGCAGAGCATTTAGCATGttcgctacga                                                                                   >  2:383871/1‑244 (MQ=18)
                                                                                                         cccttcacgctgattaccagtcatccGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCTTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCTCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACTCCCTGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttc                                                                         <  1:370172/226‑1 (MQ=25)
                                                                                                          gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                                                                                     >  2:241154/1‑222 (MQ=255)
                                                                                                          gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCCCCTTTTGCTTGTTCTCTTCGATAAAACcttct                                                                        >  1:1005495/1‑251 (MQ=255)
                                                                                                                cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCg                                                                  >  2:1065093/1‑251 (MQ=255)
                                                                                                                   gtgttACCAGGCCTACGCCCGACAGTGTAGTCGCCTGAGTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑CGTCGGC‑CACCG‑CAGTAATGGCTGATGAGACAG‑TGGTCGCGTCTTATCAGGCCTATCATATCCGTTGGTTAATTTT‑TCACACTCGTCACGCCCGGTATCAAAACCTTCCATACAGCTAAAGTTCGACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTACACCGttt                                                               <  1:1515235/248‑1 (MQ=255)
                                                                                                                     cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACTGTTGGTTAATTTT‑TCAACCTCTTCACAcc                                                                                                                                                     >  2:846774/1‑163 (MQ=255)
                                                                                                                        agcAGGCGTAGGCGAGACAGCGCAATAGCCTGATTTAGCTGGATTTTGTAGGTCGGGTAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGaa                                                          <  2:1775325/249‑1 (MQ=37)
                                                                                                                        aTCAGGCTGACTCCAGACAGCGAAAGAGCCGTATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTGTCG‑‑C‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TGGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCACAAGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGaa                                                          <  1:628561/251‑1 (MQ=255)
                                                                                                                        aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGaa                                                          <  2:661831/251‑1 (MQ=255)
                                                                                                                          cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAca                                                        <  2:1517922/251‑1 (MQ=255)
                                                                                                                              ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcatcat                                                    <  2:1615417/251‑1 (MQ=255)
                                                                                                                               ttACGCCAGGAAGCGCAATAGCATGATTTAGCGTGAGTTTTTAGGTAGGAGAATGCGTT‑T‑ACGC‑‑C‑GG‑AACCGGAATCAATGCCTGGTGCGGCGA‑TTGCCGCGTCTTATCAGGCTTATCTTAACGTTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCGTCCATACAGCTCAGGTTTAACCAGGGCAGCACCTTTTGCGTGGTCTTCTCGATAAAACCTTCTGCACCGTTTAAGAAcatcatc                                                   <  1:1437027/250‑2 (MQ=255)
                                                                                                                               cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGGTAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCAtt                                                   <  1:240563/251‑1 (MQ=255)
                                                                                                                                   gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGCCGC‑TTGCAGCGTATTATCAGGCCCATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                >  2:1369081/1‑170 (MQ=18)
                                                                                                                                   gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCCTCTTCACCGTTTAAGAACATCATTCCCg                                               >  1:1615380/1‑251 (MQ=255)
                                                                                                                                       gACATCGCAGTAACCGTATTTAGCGTGGTTTTGTAGCTCGGATAACGCGTT‑T‑ACGC‑‑C‑GC‑ATTCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCATTGGTTAATTTT‑TCAAACTCTTCGCACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCCACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCa                                           <  1:488091/251‑1 (MQ=37)
                                                                                                                                        aCAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTAGTTTATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTACTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAg                                          <  1:1923165/251‑1 (MQ=255)
                                                                                                                                        aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAg                                          <  2:557393/251‑1 (MQ=255)
                                                                                                                                          aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTa                                        >  2:1018799/1‑251 (MQ=255)
                                                                                                                                              cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                                    <  2:185683/219‑1 (MQ=255)
                                                                                                                                                  aGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAAt                                <  1:601487/251‑1 (MQ=255)
                                                                                                                                                    ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTCTCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCCCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTCCTTCACAGTTTAAGAACATCATTCCCGCCTAGTACTCGGAATaa                              >  1:1245595/1‑251 (MQ=255)
                                                                                                                                                    ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCCTCCATACAGCCCAGGTTCAACCAGTGCAGCGCCTTTTGCTTTTTCTTCTCGATAAAACCTTCTTCACCGTTCAAGACCATAATTCACGCCCACTACTTGCAATac                              >  1:1321734/1‑250 (MQ=25)
                                                                                                                                                    ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTGCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATaa                              <  2:453682/251‑1 (MQ=255)
                                                                                                                                                       gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTc                                     <  1:1058728/241‑1 (MQ=255)
                                                                                                                                                         tttAGCGTGATTTTGTAGGTCGGCTAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTCCACCGTTTAAGAACATCATTCCCGCCCAGTCCTCGGAATAACCGGt                         >  2:1864061/1‑251 (MQ=255)
                                                                                                                                                         tttAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGTCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTATTTTT‑TCAAACTCTTCATACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACTTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGt                         >  2:1034239/1‑251 (MQ=255)
                                                                                                                                                         tttAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAAATCATTCCCGCCCAGTACTCGGAATAACCgtt                         >  1:1832476/1‑249 (MQ=255)
                                                                                                                                                         tttAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGt                         >  2:1908273/1‑251 (MQ=255)
                                                                                                                                                         tttAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑C‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TAAAACTATTCACACCCGGTATCAAACCATTCAATACAGCCCAGGTTCAACCAGTGCAGCGCCTTTTCCTTGTTCTTATCGATCAAACCTTCTTCACCGTTTGAGAACATCCTTCCCGGCCAGTACTCGGAATacacgct                         >  2:951892/1‑245 (MQ=25)
                                                                                                                                                         tttAGCGTGATCTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGTGTCTTATCAGGCCTATCTTAATCGTTGGTTAATTTT‑TCAAACTATTAACACCCGGTATCAAAACCTTCCATACAGCCCAGGTTCAACCAGTGCAACGGCATTTGCTTGATCTTTTTGATAAAACCTTCTTCACCGTTTAAGAAcatcat                                                    >  1:1562012/1‑224 (MQ=25)
                                                                                                                                                            aGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCg                      <  1:1319240/251‑1 (MQ=255)
                                                                                                                                                            aGCGTGATTTTGTAGGTCAGATAAGCCGTT‑C‑A‑‑CA‑CAGC‑ACCTGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTAATTTT‑TCATACTCTTCACACCCGTTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCTCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCg                      <  2:1289278/251‑1 (MQ=38)
                                                                                                                                                                 gATTTTGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCCTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTCCCATACAGATCAGGTTCCATCAGTGCAGCGCCTTTCGCTTGTTCTTCCCGATCAAACCTTCTTCACCGTTTACGAACACCATTCCCGCCCATTACTCGGCATAACCGGTTGTGGaaa                 >  1:1838601/1‑251 (MQ=37)
                                                                                                                                                                   ttttGTAGGTCGGATAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAAtt               >  2:12988/1‑251 (MQ=255)
                                                                                                                                                                         atggctgATAAGTCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTTATTCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTACTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCagag         <  1:954983/245‑1 (MQ=255)
                                                                                                                                                                               gATAAGGCGTT‑T‑A‑‑CGGC‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAGTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTACAGAGCTGCGt   <  1:1320265/251‑1 (MQ=255)
                                                                                                                                                                                aTAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGtt  <  2:1005502/251‑1 (MQ=255)
                                                                                                                                                                                 tAAGGCGTT‑T‑ACGC‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcatcat                                                    <  2:1414074/200‑1 (MQ=255)
                                                                                                                                                                                              ||                                                                                                                                                                                                                                                   
GCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑T‑A‑‑C‑‑C‑GC‑ATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTT‑TCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTT  >  NC_000913/4296193‑4296617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑