Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,809 G→T G1189C (GGT→TGT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,8090GT98.7% 523.7 / NA 154G1189C (GGT→TGT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (72/78);  total (74/80)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

TATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTT  >  NC_000913/4184562‑4185053
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
tATGAACATCGGTCAGATCATCGAAACCACCCTGGGTATGGCTGGAAAAGGTATCGGCGAAAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTTGGCGAATTCTTCCAGCGTGCGTACGATCTGGGCGCTGCCGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGTCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACtgtg                                                                                                                                                                                                                                                   <  1:1136063/251‑1 (MQ=255)
         cGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAaagaag                                                                                                                                                                                                                                          <  2:1565891/251‑1 (MQ=255)
          tgTCATATCCTCCAAACCCACCTGGGTAGGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCGTAAACAGCAGCAAGAAGTCGCGCAACTGCGCGAATTCATCTAGCGTGCGTACGATCTGGGCGCTGACGTTCTTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGc                                                                                                                                                                                                                                         <  1:245779/250‑1 (MQ=255)
          ggTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGc                                                                                                                                                                                                                                         >  2:870262/1‑251 (MQ=255)
               gATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGaaa                                                                                                                                                                                                                                    >  1:1007544/1‑251 (MQ=255)
                   cTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTaa                                                                                                                                                                                                                                >  2:1571574/1‑251 (MQ=255)
                       aaaCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGAGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATAAAcgag                                                                                                                                                                                                                            >  1:502625/1‑251 (MQ=255)
                       aaaCCCACCTGGGTATGGCGGCGAAAGGTATCGGCGAAAAGAGCAACGCCATGCTGAAACAGCAGCAAGGAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAagag                                                                                                                                                                                                                            <  1:1451011/251‑1 (MQ=255)
                                 gggTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAAc                                                                                                                                                                                                                  <  2:1062386/251‑1 (MQ=255)
                                   gTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCAATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCCGCGTGAGTACGATCTGGGCGCTGACGTTAGTCAGATAGTTGACCTGAGTACCTTCCGCGATGAAGAAGTTATGCGTCTGGTTGCAAACCTGCGCAAAGGTATGCAACGCGCAACGACGGGGTTCGACTGTGCGAAAGACGCAGAAATTAAAGAGTGGGTGAAACtt                                                                                                                                                                                                                >  1:44063/1‑251 (MQ=255)
                                    tATGGCTGCGAAAGGTAGCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTg                                                                                                                                                                                                               <  1:1185576/251‑1 (MQ=255)
                                       ggCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCg                                                                                                                                                                                                            >  2:504269/1‑251 (MQ=255)
                                               aaGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTACGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCAGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCg                                                                                                                                                                                                    <  2:234510/251‑1 (MQ=255)
                                                    aTCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGACATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                                                                                                                                               >  1:988092/1‑251 (MQ=255)
                                                    aTCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                                                                                                                                               <  2:1404799/251‑1 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  1:317096/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  2:360772/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  1:582171/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  2:1438521/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  2:912445/1‑251 (MQ=255)
                                                          gACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCa                                                                                                                                                                                         <  1:1183547/251‑1 (MQ=255)
                                                               gATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCa                                                                                                                                                                                         <  2:1253847/246‑1 (MQ=255)
                                                               gATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATcc                                                                                                                                                                                    <  2:392188/251‑1 (MQ=255)
                                                               gATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATcc                                                                                                                                                                                    <  2:97924/251‑1 (MQ=255)
                                                                  cAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCTGCTTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 <  2:165865/251‑1 (MQ=255)
                                                                  cAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCGGCGGGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 <  1:1571874/251‑1 (MQ=255)
                                                                  cAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 <  2:696989/251‑1 (MQ=255)
                                                                  cAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACATTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGACGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTGGGCGACATGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 >  2:85984/1‑251 (MQ=255)
                                                                    acaccATGCTGAAACAGGAGCAAAAGAGCGCGGATCGCCGATTATTCATCCAAGGGGCGTACGATTTGGGCGCTTCCGTTTGGCATAAAGTAGACCCGAGTCCCGTCAGCCCTGAAGAAGTTATGCGGCTGGCTGAAGACCTGCGCTAAGGCATGCCGATCGCAACGCCCGTGTTAGACTCAGCGATAGAAGCAGAAATTAAAGAGCTGCTGAAACCTGGCGATCTGCTGATTTCCGGTCAGCTCCGCCTg                                                                                                                                                                               <  1:91998/248‑1 (MQ=255)
                                                                     cGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTAAGCCAATCGCAACGCCGGGGTGCGACTGTGCGACAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGCTCAGATCAGCCTga                                                                                                                                                                              >  1:888723/1‑250 (MQ=255)
                                                                         aTGCTGAACCATCAGCAATAAGTCGCGAAACTGCGCGAATTCATCCAGCGCGCGTACGATTAGGGCGCTGACGTTCGTCAGACTGGTTCGCTGAGTACCTTCAGCGAATAAGAGGTTATACGTCTGGCAGAAAATCTGCGCACAGTGAGCCTAATCGCAACACGGGTTTTCGACCGCGCGAACGACGCAGAAAGTAAGGCGCCGCGGAAACATGGCGCACTGCTGCCTTACGGGTAGATCCGTCTGtgaga                                                                                                                                                                          >  1:1559400/1‑247 (MQ=255)
                                                                         aTGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGa                                                                                                                                                                          >  2:1495659/1‑251 (MQ=255)
                                                                          tGCTGGAACAGCAGCTAGAAGTCGCGAAACTGAGCGAATTCATCCAGCGTGCGTTAGGTCTGGGGGCTGGCTTTCATCCAAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGGTATGCGTCTGGCTGAAGACCTGCGCAAAGGTAGGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTTGCGACCTGCCGACTTCCGGTCAGATCCGCCTATACGAt                                                                                                                                                                         <  2:649586/251‑1 (MQ=255)
                                                                                    gcagcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCa                                                                                                                                                                                         <  2:1399166/225‑1 (MQ=255)
                                                                                    gcagcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGACAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCGGATCCGCCTGTACGATGGTCGCACTg                                                                                                                                                               >  2:382370/1‑251 (MQ=255)
                                                                                       gcaAGAAGTCGCGAAGCTGCGCGAATTTATCCAGCGTGCGTACGAGCTGAGCGCTGACGTTCGACAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGGATACCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCGGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTg                                                                                                                                                            <  2:1296192/251‑1 (MQ=255)
                                                                                       gcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGCAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTg                                                                                                                                                            >  2:933297/1‑251 (MQ=255)
                                                                                       gcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTg                                                                                                                                                            >  1:697171/1‑251 (MQ=255)
                                                                                       gcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGAACCGCCTGTACGATGGCCGAAcctgtc                                                                                                                                                            >  1:1254102/1‑246 (MQ=255)
                                                                                               ttggtaaaCTGGGTGAGTTCATCGAGCGTGGTTACGATTTGGGAGCTAGCTTGCGTGAGAAAGTTGACTTGATGACCGTCAGCGAGGAAGAGGTTATGCGTCTGGCTGAGAACCTGCGCAAAGGTATACCAATCGCAACGCAGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTc                                                                                                                                                    <  1:218002/246‑1 (MQ=255)
                                                                                                  cgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGCCGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                                                                                                                                 <  1:321122/251‑1 (MQ=255)
                                                                                                   gAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGCCGCACTGGGAAACAGTTCGAGc                                                                                                                                                >  1:1372967/1‑251 (MQ=255)
                                                                                                   gAAACTGCGCGAATTCATCAAGCGTGCGTAGGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGGTGAAGAAGTTATTCGTCTGGCTGAAAACCTGCGCAAAGGTAAGCCAATCGCCACGCCGGTGTCCGACTGTGCGAAAGAAGCAGAAAGTAAAAACCTGCTGAAACTTGACGACCTGCCGACTTCCAGTCAAGACCGCCTGTCCGATGGTCGCACTGGCGACCCGTTCgcgc                                                                                                                                                >  1:826015/1‑251 (MQ=255)
                                                                                                     aaCTGCGAGAAGTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAGCCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGt                                                                                                                                              <  2:1229319/251‑1 (MQ=255)
                                                                                                       cTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTcc                                                                                                                                            <  2:86243/251‑1 (MQ=255)
                                                                                                         gcgcGAATTCATCCAGCGTGCGTACGATCTGGGCGCGGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCgg                                                                                                                                          <  1:1278848/251‑1 (MQ=255)
                                                                                                              aaTTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAgctgct                                                                                                                                                                                                                       <  1:450846/169‑1 (MQ=255)
                                                                                                               aTTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCg                                                                                                                                    <  2:151097/251‑1 (MQ=255)
                                                                                                               aTTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCg                                                                                                                                    >  2:321029/1‑251 (MQ=255)
                                                                                                                    tcacccgtgcgtACTATCTGGGCGCTGGCGGTGGTCAGAAAGTTGACCTGAGTACCTTCAGCAGTGAAGAAGTTATGCGTCGGGCTGAAATCCTGCGAAAAGGTATGCCAATCGCGACGCCGGTGTTCGAGTGTTAGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACGTGCCGAGTTCCGGTCAGATACGCCTGTACGATGGTCGCACTGGTGGACAGTTCGGGCGTCCGGTAACCGTTGGt                                                                                                                               <  2:1271/246‑1 (MQ=255)
                                                                                                                      cAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTCCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTCCGAAAGAAGCAGAAATTAAAGAGCTCCTGAAACTGGGCGACCTGCCGAATTCCGGTCAGACCGCCCTGAACGAGGTCCGCATTGGCGACCAGTTCGAGTGCCCgtcacccgtgggcta                                                                                                                             >  1:563856/1‑237 (MQ=255)
                                                                                                                                      tGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTc                                                                                                                                                                                               >  2:450723/1‑169 (MQ=255)
                                                                                                                                          cgcTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGAACCGCCTGTACGa                                                                                                                                                                          >  1:1002034/1‑186 (MQ=255)
                                                                                                                                          cgcTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAAATAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGa                                                                                                         >  1:529805/1‑251 (MQ=255)
                                                                                                                                           gcTGACGTTCGTCAGAAAATTAACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGaa                                                                                                        <  2:845231/251‑1 (MQ=255)
                                                                                                                                                cGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGGGAACAGTTCGAGCGTCCGGTCACCGTTGGTTACATGTACATGCTGAAACTGAaccccc                                                                                                   >  1:1482504/1‑251 (MQ=255)
                                                                                                                                                  ttCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTg                                                                                                 >  2:1487695/1‑251 (MQ=255)
                                                                                                                                                     gTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTc                                                                                              >  2:1315886/1‑251 (MQ=255)
                                                                                                                                                     gTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTc                                                                                              >  2:934740/1‑251 (MQ=255)
                                                                                                                                                          aaaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacga                                                                                         <  2:1176604/251‑1 (MQ=255)
                                                                                                                                                          aaaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacga                                                                                         <  2:1372703/251‑1 (MQ=255)
                                                                                                                                                                 acgtggatcccTTCAGCGATTAAGAAGTTATGCGTCTGTCTTAAAACCAGCGCAAAGGTCTGCCAATCGCCACGCCGGTGTTCGACTGTGGGAAAGAAGCAGAAATTAAAAGGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  1:944317/242‑1 (MQ=255)
                                                                                                                                                                 aCCTGGGTACCTTCAACGAGGAAGAAGTTAGGCGTCTGGCTTAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGGGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTGCGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  2:563705/251‑1 (MQ=255)
                                                                                                                                                                       gTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                                                                                                                                 <  2:1508238/182‑1 (MQ=255)
                                                                                                                                                                       gTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACCACAAGATGCAcgcg                                                                            >  1:1034271/1‑251 (MQ=255)
                                                                                                                                                                        gtcctttggaggagaagagGTTATGCGTGTGGCTTGACACCTGAGCAGAGGTATGCGAATCGGAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAATTAGCTGCTGAGACTTGGCGGCCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGGTGAAACTGAACCACCTGGTCGACGACAAGATGCAcgcgc                                                                           <  1:781427/238‑1 (MQ=255)
                                                                                                                                                                           cTTCAGCGATGAAGAAGTTAGGCGTCGGGCGTAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAAGTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGtt                                                                        <  1:870463/251‑1 (MQ=255)
                                                                                                                                                                                   aTGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCAcg                                                                              <  2:322590/237‑1 (MQ=255)
                                                                                                                                                                                        gaagTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTa                                                           >  1:27653/1‑251 (MQ=255)
                                                                                                                                                                                            ttATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGGGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGGGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGc                                                       <  1:678464/251‑1 (MQ=255)
                                                                                                                                                                                             tATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGCCGACAAGATGCACGCGCGTCCCACCGGTTCTTCCAGcc                                                      >  1:1507988/1‑251 (MQ=255)
                                                                                                                                                                                             tATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGCTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCGACCGGTTCTTACAGcc                                                      >  2:410476/1‑251 (MQ=255)
                                                                                                                                                                                             tATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTCCGAAAGAAGCAGAAATCAAAGAGCTGCTGAAACCTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTTAACAGTTCGAGCGTCGGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACCAGATGCCAGCGACTTCCACCGGTTCTTaacgac                                                      >  1:532306/1‑246 (MQ=255)
                                                                                                                                                                                               tGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatg                                                                                                                         >  1:1508528/1‑182 (MQ=255)
                                                                                                                                                                                                 cGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGAAGAAATTAAAGAGCTGCTGAAAATTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTAAGATGGTCGAACTGGTGTACAGTTCGAGCGTCCGGTACACGTTGGTTACATGTAAACGCTGAACCTGACCCACATGGTCGACGCCACGAGGCACGAGCGTTCCACCTGTTCTGACAGctgggt                                                  >  1:1059450/1‑246 (MQ=255)
                                                                                                                                                                                                 cGTCTGGCTGAAAACCTGCGCAAAGGTAAGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt                                                  <  2:502491/251‑1 (MQ=255)
                                                                                                                                                                                                    cTGGCTGAAACCCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTAc                                               <  2:1216752/251‑1 (MQ=255)
                                                                                                                                                                                                      ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacg                                                                                          <  2:925055/206‑1 (MQ=255)
                                                                                                                                                                                                      ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTc                                             <  1:548819/251‑1 (MQ=255)
                                                                                                                                                                                                       gCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGATCAGt                                                                                                                                                      <  2:59049/145‑1 (MQ=255)
                                                                                                                                                                                                       gCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgta                                                                                                                       <  2:42471/176‑1 (MQ=255)
                                                                                                                                                                                                        cTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcag                                           >  2:1007815/1‑251 (MQ=255)
                                                                                                                                                                                                           aaaaCCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCGGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGcc                                                      >  1:322683/1‑237 (MQ=255)
                                                                                                                                                                                                           aaaaCCTGCGCAAAGGTATGCCAATCGCAAAGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGCTACTccgcag                                        >  2:46744/1‑251 (MQ=255)
                                                                                                                                                                                                           aaaaCCTGCGAAAAGGTATGCCAATCCCAACGCCGGTGTTCGGCTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcagcag                                        <  1:360877/251‑1 (MQ=255)
                                                                                                                                                                                                                  gcgcAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGt                                                                                                                                                      <  2:5753/134‑1 (MQ=255)
                                                                                                                                                                                                                  acgcAAAGGTCAGCCCATGCGAACGGCGGTGTGCGAAGGGGGGAAAGAAACAGAAAAAAAAGAGCTGCTGAAACTGGTCGACCTACAGAACTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTGGGTTACATGTACATGCTGAAACTGAACCACCTTGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTgg                                 <  1:173541/250‑1 (MQ=255)
                                                                                                                                                                                                                         ggTATGCCAAACGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATGAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCt                                   <  1:147078/242‑1 (MQ=255)
                                                                                                                                                                                                                             tGCCAATCCCAACGCAGGTTTTCGACTGTGCAAAAAAAGCATAAATTAAAGAGCTGCTGAAACTTGGGGACCTGCCGACTTCCGGTCAGATCAGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGGCCGGTAACCTTTGGTTACATTTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCTCGTTCCTCCGGTTCTTCCAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGca                      <  2:825820/251‑1 (MQ=255)
                                                                                                                                                                                                                              gCCAATCGCAACGCCGGTGTTAGAGTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGCCCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGcac                     <  2:1079445/251‑1 (MQ=255)
                                                                                                                                                                                                                               ccAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGATCAGTTCGAGCGTCCGGTAACCGTTGGtt                                                                                                                              >  1:59111/1‑145 (MQ=255)
                                                                                                                                                                                                                               ccAATCGCAACGCCGGCGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGACCAGTTCGCGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATCCAGGCGCGTTACACCGGTTCTTACAGCCTGGGTACTCAGCAGACGCTGGGTGGTACGCcaca                    >  1:1144549/1‑251 (MQ=255)
                                                                                                                                                                                                                                cAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAg                   >  1:840579/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACATCAGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACAGGTACATGCTGAAACTGAACCACCTGGTCGACGCCAAGATGCACGCGCCTTCCACCGGTTCTGACAGACCGGTTACTCAGCAGCCGCCGGGTGGCAAGCCACAGTTCggcggta         >  1:220289/1‑250 (MQ=255)
                                                                                                                                                                                                                                            cGGGGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCCGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAg       <  1:1438801/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gtgtTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCAcc                                                                   <  2:121018/189‑1 (MQ=255)
                                                                                                                                                                                                                                               tgtTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGt    <  2:276767/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCggtgg           >  2:147012/1‑242 (MQ=255)
                                                                                                                                                                                                                                                 ttCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGttt  >  2:753313/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 ttCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGGCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGttt  >  2:1169191/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
TATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTT  >  NC_000913/4184562‑4185053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑