mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | gctrim-C13-Redo-3-57_S3_L001_R2_001 | 1,584,165 | 374,331,150 | 100.0% | 236.3 bases | 251 bases | 86.8% |
| errors | gctrim-C13-Redo-3-57_S3_L001_R1_001 | 1,583,868 | 370,557,822 | 100.0% | 234.0 bases | 251 bases | 98.1% |
| total | 3,168,033 | 744,888,972 | 100.0% | 235.1 bases | 251 bases | 92.5% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 149.3 | 4.8 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 8780 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 44 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.005 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.82408 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 06:07:48 26 Jun 2016 | 06:08:54 26 Jun 2016 | 1 minute 6 seconds |
| Read alignment to reference genome | 06:08:54 26 Jun 2016 | 06:13:04 26 Jun 2016 | 4 minutes 10 seconds |
| Preprocessing alignments for candidate junction identification | 06:13:04 26 Jun 2016 | 06:14:26 26 Jun 2016 | 1 minute 22 seconds |
| Preliminary analysis of coverage distribution | 06:14:26 26 Jun 2016 | 06:18:22 26 Jun 2016 | 3 minutes 56 seconds |
| Identifying junction candidates | 06:18:22 26 Jun 2016 | 06:18:24 26 Jun 2016 | 2 seconds |
| Re-alignment to junction candidates | 06:18:24 26 Jun 2016 | 06:19:02 26 Jun 2016 | 38 seconds |
| Resolving alignments with junction candidates | 06:19:02 26 Jun 2016 | 06:22:06 26 Jun 2016 | 3 minutes 4 seconds |
| Creating BAM files | 06:22:06 26 Jun 2016 | 06:24:34 26 Jun 2016 | 2 minutes 28 seconds |
| Tabulating error counts | 06:24:34 26 Jun 2016 | 06:28:16 26 Jun 2016 | 3 minutes 42 seconds |
| Re-calibrating base error rates | 06:28:16 26 Jun 2016 | 06:28:17 26 Jun 2016 | 1 second |
| Examining read alignment evidence | 06:28:17 26 Jun 2016 | 06:55:50 26 Jun 2016 | 27 minutes 33 seconds |
| Polymorphism statistics | 06:55:50 26 Jun 2016 | 06:55:50 26 Jun 2016 | 0 seconds |
| Output | 06:55:50 26 Jun 2016 | 06:56:27 26 Jun 2016 | 37 seconds |
| Total | 48 minutes 39 seconds | ||
