New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 350561374 (0.720)4 (0.040) 4/438 8.8 5.8% coding (408/1086 nt) yhfS FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
?NC_000913 3505821 = 62 (0.650)coding (200/1086 nt) yhfS FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGC  >  NC_000913/3505364‑3505862
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
cGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTAACAGCCGATTCGAGCCACCTTCATCACGGCATAGTTATCATCGGTTAACGCTGGAACACCCGCCGCGCGCAACGTTGCCAGCAACTCTCCCACAACGTCGCTGTGCTGCGGGCGGTGGCGCGTAAGCGGCACCCGCGCCGCAt                                                                                                                                                                                                                                                          >  2:816941/1‑251 (MQ=255)
     cgcgAATACGGTTGATAACATCAGCACTGCCAGCCACTGAACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGGGGCATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAATTATCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGgt                                                                                                                                                                                                                                                     <  1:752684/251‑1 (MQ=255)
                ctGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGCTGTCATCGGTTAAAGCTCGAACACCTGCCGCGCGCAACGTTGCCAGCACCTCTGCCAGCACGTCGCTGTCCTGCGGCTGCTGGCGCGTATGTCGCACCAGCGCCCTATCTGGTTGATGCtcgtc                                                                                                                                                                                                                                          >  2:552911/2‑251 (MQ=255)
                 tGATAACACCAGCATCGCCTACCCCTGCACCAACAGCCTCAGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCCATTCGCGCCACCTTCATCTCCGCATAGTTGTCATCGGTTACCGCTGGAACACCTGCCGCACGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCATGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTACtcgtcg                                                                                                                                                                                                                                         <  1:424244/251‑1 (MQ=255)
                   ttaccataagcaagcccGACACCTGCACCAACACCCGCTGGCCCAAATAGCTTGAAGCAGGAAAAGGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCCGGAACACCTGCCGCGCGCAACGTTGCCAGCACAGCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTcgac                                                                                                                                                                                                                                       <  2:571264/236‑1 (MQ=255)
                   aTAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGCGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCGGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTcgac                                                                                                                                                                                                                                       <  1:617452/251‑1 (MQ=255)
                    tAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGCTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCCCATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCCCGTcgacg                                                                                                                                                                                                                                      >  1:158338/1‑251 (MQ=255)
                               tcaccgccccccggaacttcacCCGCTGTCCCACATGGCTTGAGGCAGCAACATGTCGAGACATTCGGGCCCCATGCACATCCGGTTCGAGCCACCTTCATCTCCGCATAATTGTCATCGGTAAACACTGGACCACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTACCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCGGCGCCGCATCCGGTTGTTGCTCGTCGACAACCTGCTTCaa                                                                                                                                                                                                                           <  2:975652/232‑2 (MQ=255)
                                 gCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTg                                                                                                                                                                                                                         <  1:201391/251‑1 (MQ=255)
                                      ccACTCCACCAACCCCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGAc                                                                                                                                                                                                                    <  1:701548/251‑1 (MQ=255)
                                      ccACCGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCTAGACCTTCGCCCCGCATTCACAGCCGATTCGAGCCCCCTTCATCACCGCTTAGTTTTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTTTTGCTCGTCGACGACCTGCTTCAGTGCCGAc                                                                                                                                                                                                                    <  2:783635/251‑1 (MQ=255)
                                         cggCACCAACACGCCCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCCCCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAgg                                                                                                                                                                                                                 <  2:1073167/249‑1 (MQ=255)
                                          ttcACCAACACCCGCTGTCCCAAATAGCTTGAAGCAGGAAAATGTCGACACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGt                                                                                                                                                                                                                <  1:609710/249‑1 (MQ=255)
                                          tGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGATAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATCACGCACCACCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGATTCAGTGcgtccaggt                                                                                                                                                                                                                >  1:777531/1‑243 (MQ=255)
                                              ccAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCAtt                                                                                                                                                                                                            >  1:963189/1‑251 (MQ=255)
                                               cAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCCTCCGGTTGTTGCTGGTCGACGACCTGCTTCAGTGCAGACAGGTCATTg                                                                                                                                                                                                           >  1:808833/1‑251 (MQ=255)
                                               cAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCACCCGGTTGTTGCTCGTCGACGACCTGCCTCAGTGCCGACAGGTCATTg                                                                                                                                                                                                           >  1:480730/1‑251 (MQ=255)
                                                 aggacaccgggCCCAAATGGTTTGAAGCAGGAAAGGGGCGTGACATTCGGGCCGCATTCACGGCGGATTCGCGCCACCTTCATCACCGCGGAGTGTTCCTCGAGGAACGTGGGACCACCGGCCGCTCGCAACGTTGCCAGAACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGGTCGTATGCTGCGCCTGCGCCGCATCCGATTATTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGaa                                                                                                                                                                                                         <  2:762256/242‑1 (MQ=255)
                                                 acacCGTCTTGCCCAAATATGTTTAAGCAGGAAAATGTCGAGACATTCGCACCGCATTCACAGCCGATTCGAGCCACCTTCGTCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAGCGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGaa                                                                                                                                                                                                         <  1:780088/251‑1 (MQ=255)
                                                     ccTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCa                                                                                                                                                                                                     <  1:667142/251‑1 (MQ=255)
                                                       tctGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAAc                                                                                                                                                                                                   >  1:252795/1‑251 (MQ=255)
                                                       tctGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCCGGCGCGTATGCTGCACCAGCCCCGCACCCGGATGTTGCTCGGCGACGAACTGCCTCAGTGGCACCAGGCCCTTAAAAtaaca                                                                                                                                                                                                   >  2:906810/1‑247 (MQ=255)
                                                        ctGGCCCAAATAGCTTGAAGCAGGAAAAGTTCGGGACATTCGGGCCGCTTTCACAGCCGATTCGAGCCACCTTCATCCCCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCGGCGCGCATCGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACa                                                                                                                                                                                                  <  2:252846/251‑1 (MQ=255)
                                                         tGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCCTCAGTGCCGACAGGTCATTGAAATCAACAg                                                                                                                                                                                                 >  1:783498/1‑251 (MQ=255)
                                                              cAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCCGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCCGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCCGTGCCGAAAGGCCATTGAAATCAACAGtaata                                                                                                                                                                                            >  1:833099/1‑251 (MQ=255)
                                                               aactAGCTGAAAGCAGGAAAATGTCGCGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGtaataa                                                                                                                                                                                           <  1:34880/248‑1 (MQ=255)
                                                               aaaTAGCTTGATGCATGAACATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCGGCCGCGCGCAACGTTGCCAGCACATCTGGCAGCACGTAGCTGTCCTGCGTCTGCTGGCGCGTATGCTGCGCCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGCGCCGACAAGTCATTGAAATCAACAGtaataa                                                                                                                                                                                           <  1:881146/251‑1 (MQ=255)
                                                               aaaTAGCTTGAATCAGTAAAATATCGAGACATTCGCTCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCCGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGtaataa                                                                                                                                                                                           <  2:833244/251‑1 (MQ=255)
                                                               aaaTAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGtaataa                                                                                                                                                                                           <  1:239324/251‑1 (MQ=255)
                                                               aaaGAGCTTGAAGCAGGAAAATGTGGAGTCATTCGAGCCGCATTCACAGCCGGTTCGAACCACCTACATCACCACATAATTGTCACCGGTTAACGCTGAAACACCTGCCGCGCGCAACGTTGCCAGCACATCTTCCAGCACGTAGCTGTCCTGCGGCTACTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTATTGCTCGTCGACGACCTGCTTCAGTGACGACAGGTCATTGAAATCAACAGtaataa                                                                                                                                                                                           <  1:392828/251‑1 (MQ=255)
                                                                   aGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGACGCATCCGGTTGTTACCCGTCGACGACCTGCTTCAGTGCCGACAGGTCATCGAAATCAACAGTAATAAGCGt                                                                                                                                                                                       >  1:848317/1‑251 (MQ=255)
                                                                    gcgtGAAGAAGGAAAATGTCGAGACATTCGCGCCGCATTCCCAGCCGATTCGAGCCCCCTTCATCACCGCATAGTTCTCATCGGTTAACGCTGGAACACCGGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTc                                                                                                                                                                                      <  2:480840/248‑1 (MQ=255)
                                                                    gCTTGAAGGAGGCAAATGTCGAGTCATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAGATCAACAGTAATAAGCGTc                                                                                                                                                                                      <  1:115296/251‑1 (MQ=255)
                                                                    gCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCCTCAGTGCCGACAGGGCCTTGAAATCAAAAGTAATAAGCGTc                                                                                                                                                                                      >  2:239375/1‑251 (MQ=255)
                                                                    gCTTGAACCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTc                                                                                                                                                                                      <  1:27630/251‑1 (MQ=255)
                                                                      ttGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTAATTGAAATCAACAGTAATAAGCGTCAg                                                                                                                                                                                    >  1:8379/1‑251 (MQ=255)
                                                                         caGCATGTAAATTTCGAGACATTCTCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGATCATTGAAATCAACAGTAATAAGCGTCAGccc                                                                                                                                                                                 <  1:1081194/250‑1 (MQ=255)
                                                                         aaGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTAGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCACCCGGTTGTTGCTCGTCGACCACCTGCTTCAGTGCCGACGGGTCAATGAAATCAACGGTAATAAGCGACAGccc                                                                                                                                                                                 >  1:608788/1‑251 (MQ=255)
                                                                             aGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCCTCAGTTCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATc                                                                                                                                                                             >  2:361954/1‑251 (MQ=255)
                                                                                gagacgTCGTGACATTCGCGCCGCAATCACAGCCGGTTCCGTCCACCTTCATCACCGCATAGTTGTCTTCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCGAGCACATCGGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGc                                                                                                                                                                          <  1:747437/246‑1 (MQ=255)
                                                                                     gTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCaat                                                                                                                                                                     <  1:361878/251‑1 (MQ=255)
                                                                                     gTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAACAGTCGGCCCCATCTGCCCaat                                                                                                                                                                     >  1:910267/1‑251 (MQ=255)
                                                                                        gagaCATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCCTAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCaataat                                                                                                                                                                  <  1:816795/251‑1 (MQ=255)
                                                                                                      gcATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTTCCGACCGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCAGTGtcgtcg                                                                                                                                                    >  1:1034334/1‑251 (MQ=255)
                                                                                                          tCACAGCCGATTCGAACCACCTTCATCACCGCATAGTTGTCATCGGTTAGCGCTGGAACACCTGCCGCGCGCAACGGTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCGGGCGCGTATGCTGCACCAGCGCAGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTa                                                                                                                                                <  1:552797/251‑1 (MQ=255)
                                                                                                                        aGCCCCCTTCATCACCGCTTAGTTGTCATCGGTTAACGCTTGAACACCTGCCTCCGGCAACGTTGCCAGCACATCTGCCAGCACATAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCGTCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCAt                                                                                                                                  <  2:370725/251‑1 (MQ=255)
                                                                                                                        aGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACTCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCAt                                                                                                                                  <  2:1034498/251‑1 (MQ=255)
                                                                                                                             ccTTCATCACCGCATACTTGTCATCGGTTAACGCTAGAACCCCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTTTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCACGTCAt                                                                                                                                  <  1:947174/246‑1 (MQ=255)
                                                                                                                               ttCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCAGCCGCGCGCAACGTTGCCAGCACATCCGCTAGCACGTAGCTGTCCTGCGCCCGCTGGCGCGTCTGCCGTACACGCTCCGCATCAGGGTGTGGCTCGTCGACGACCTGCTCCATTGCAGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATACGCCCAATAATAAAGCGGGTCGTCGGGTAAACAGGCTCGTCATGCAACag                                                                                                                           >  2:539667/1‑251 (MQ=255)
                                                                                                                                 catcaCCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCAAATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTCTGCTGCACCAGCTCCGCATCCGGTTGTTGAGCGTAGAAGACCTGCTCCCGTGCCGTCACGGCATTGAGATCAACAGCAATAAGCGTCCGACCCATCTGCTCAAAAATAACCCGTCTCGTCGGGTAACAAGGCGCGTACTGCACCagat                                                                                                                         >  2:629495/1‑250 (MQ=255)
                                                                                                                                             gTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCAACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTgtcgtc                                                                                                                                                     <  1:428798/211‑1 (MQ=255)
                                                                                                                                                 tCATCGGTTAACGCTGAAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCAcc                                                                                                                             <  1:888017/231‑1 (MQ=255)
                                                                                                                                                    tCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATACCCCGTGTCGTCGGGTAGACAGGCGCGTCATGCACCAGAAGACGCTCCCCCGGCTTGAg                                                                                                      >  1:73902/1‑251 (MQ=255)
                                                                                                                                                    tCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGATGCTGGCGCGTACGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGCCCTGCTCCAGTGCCGACAGGTCATTGAAACCAACAGTAATAACCGTCAGCCCCATCTGCTCAATAATAACCTGTGTCGCCGGGTAACAAGGCGCGTCATGCCCCAGAAGACCCCCCCCCGGTCTGAg                                                                                                      >  2:408313/1‑251 (MQ=255)
                                                                                                                                                     cGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGAGGACCTGCTTCAGTGCCGACAGGGCATCGAAATCAAAAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCAGGCTAGTCGGCTAAACAGGCACGTCATGCCCGAAAAGACGATGCCCCgggtctgtt                                                                                                     >  2:947326/1‑244 (MQ=255)
                                                                                                                                                     cGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTTAGCCACATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTTATGCACCAGAAGACGCTGCCTCGGTTTGAGc                                                                                                     >  1:229570/1‑251 (MQ=255)
                                                                                                                                                         tAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCCCCAGAAGACGCTGCCCCGGTTTGAGCAAAg                                                                                                 >  1:427545/1‑251 (MQ=255)
                                                                                                                                                           aCGCTGGAACACCTGCCGCGCGCAACGTTGCCAGTACATCTGCCAGCACGTAGCTGTTCTGCGGCTCCCGGCGCGTATGCAGCACCAGCCCCGCATCCGGTTGTTGCTCGTCGACGGACTGCTTCAGTGCCGACCGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCCGCTCACTTATAACCCGTGTACTCGGGTAAACAGGCGCGCCAAGCACCAGAATAAGCTGCCCCggctgacgcagagac                                                                                               >  2:841411/1‑238 (MQ=255)
                                                                                                                                                              cTGGAACCCGTCCCGCGCGCAACTTTGCCCGCACATCTGCAGGCACGGCGCTTTCCGGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAgccgcc                                                                                            <  2:602426/251‑1 (MQ=255)
                                                                                                                                                                  aaCACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGttgttg                                                                                                                                                                                                                                                >  1:973901/1‑99 (MQ=255)
                                                                                                                                                                         gCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCTTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGGGcctgtcgcttatacacacctg                                                                                 >  2:888164/1‑231 (MQ=255)
                                                                                                                                                                             cgcgcACCGTTGCCAGCAAATCTGCCAGCACGTGCCTGTCCTGCGGCTCCTGGCGCGTATGCAGCACCACCGCCGCATCAGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCGTCTGCTCAATAATATCCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATc                                                                             <  1:539552/251‑1 (MQ=255)
                                                                                                                                                                             agcgcAAGGTTCCCCACACATCTGCCAGCCGGTGAGTCTCCTCGGGCTACTGGCGCGTTTGCTGCACCTGCGCCGCATCCGCTTGTTGCTCGTCCACAACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACATAAATAAGCGTCAGCCCCATCTGCTCGATCATAACCCGTGTCGTCGGGTAAGCCGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATc                                                                             <  1:1082020/250‑1 (MQ=255)
                                                                                                                                                                               tggaaggTTGCCAGTACAGCCTCCGGCACGTTGCGGTCCTGCGGCTGCTGGCGCGTATGCTGCCCCAGCGCCGCATCCGGTTGTTGCGCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAGGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATcgc                                                                           <  2:182823/245‑1 (MQ=255)
                                                                                                                                                                               cgcAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATcgc                                                                           <  1:44915/251‑1 (MQ=255)
                                                                                                                                                                                    cGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATcgccgcag                                                                      >  1:957440/1‑246 (MQ=255)
                                                                                                                                                                                                      cAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCTCCATCTTCTCAATAATAACCCGTGTCGTCGGATAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCTCCAGCCCTCGCTCCCTGCACCa                                                    >  1:749516/1‑251 (MQ=255)
                                                                                                                                                                                                          acttAGCTCTCCTGCGGCTGCTGGCGCGTATGCTTCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATATTAACCCATGTCGTCGGGTAAACAGGCACGTCATGCACCGGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCAAATCGCGCCAGTccc                                                                  <  1:289307/230‑1 (MQ=255)
                                                                                                                                                                                                                  gTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGCCAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGATGCCCCGGTTTGAGAAAAGCCGCCAGCCCGGCGCGAATAGCGCCAGTCCCCGCCCCCTGCACCAGCCCCCCAGCCt                                        >  2:66791/1‑251 (MQ=255)
                                                                                                                                                                                                                   tCCTGCGGCTGCGGGCGCGTATGATGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGCCCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAAGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCtg                                       <  1:841266/251‑1 (MQ=255)
                                                                                                                                                                                                                                ggCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTg                                 <  1:994343/244‑1 (MQ=255)
                                                                                                                                                                                                                                  cgcgTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGTCAGGGCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCGGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCAACAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCGAGCCCGGCGCGAATCGCGCCAGCCACCCCCCACTGCACCACCGCCCCAGCCtgccttttttacacttc                        >  2:289364/1‑236 (MQ=255)
                                                                                                                                                                                                                                          ctgcACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCCCAATAATAACCCGTGCCGTCGGGTAAACAGGCGCGTCATGCACCCGAAGACTCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCCACCGCGCCGCAGCCTGCGCGGGAAAGGCATCAGccacca                >  2:211444/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        cATCCGGTTGTTGCTCGTCGACGACCTGCTCCAGTGCCGACAGGTCATTGAAATCAACCGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGACCAAAGCCGCCAGCCCGGCGCGAACCGCGCCATTCCCCGCGCCCTGCACCCGCCCCTACCCCTGTGAGTGAAAAGCATGAGCCAGCAACTGTccccggcgt  >  2:994505/1‑245 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
CGTGGCGCGAATACGGTTGATAACATCAGCATCGCCGACCACTGCACCAACACCCTCTGGCCCAAATAGCTTGAAGCAGGAAAATGTCGAGACATTCGCGCCGCATTCACAGCCGATTCGAGCCACCTTCATCACCGCATAGTTGTCATCGGTTAACGCTGGAACACCTGCCGCGCGCAACGTTGCCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGC  >  NC_000913/3505364‑3505862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.