Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,492,323	IS1 (–) +9 bp	coding (924‑932/1251 nt)	frc ←	formyl‑CoA transferase, NAD(P)‑binding

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	29 (0.840)	25/296	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 2492331	0 (0.000)					coding (924/1251 nt)	frc	formyl‑CoA transferase, NAD(P)‑binding
*	?	NC_000913	= 1979270	NA (NA)	29 (0.840)	20/296	0.5	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	2492323 =	0 (0.000)					coding (932/1251 nt)	frc	formyl‑CoA transferase, NAD(P)‑binding

GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979123‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCG  >  NC_000913/2492323‑2492460                                                                                                                                                                                                                                                                                                GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCA                                                                                                                                         >  2:549257/1‑151  AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTC                                                                                                                                          <  2:34863/149‑1  AGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCT                                                                                                                                           <  1:44306/148‑1   GATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAG                                                                                                                                        >  2:532845/1‑150   GATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAG                                                                                                                                        >  1:217092/1‑150                                        CACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGG                                                                                                    >  2:443705/1‑149                                          CCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGG                                                                                                    >  2:5464/1‑147                                            ACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTAC                                                                                              <  1:359993/151‑1                                              CCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTG                                                                                            <  1:283404/151‑1                                              CCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTG                                                                                            <  2:281733/151‑1                                                CGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTA                                                                                          >  1:9641/1‑151                                                  TCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAT                                                                                         <  2:355840/150‑1                                                  TCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAT                                                                                         <  1:30293/150‑1                                                     GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATTATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCG                                                                                     >  2:198611/1‑151                                                              CAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCT                                                                                                                                           <  1:520578/88‑1                                                              CAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCT                                                                                                                                           >  2:520588/1‑88                                                                  AGGTACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATGCACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCC                                                                         <  1:421660/150‑1                                                                  AGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCC                                                                         <  2:221750/150‑1                                                                                                     CCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCC                                      >  1:332179/1‑150                                                                                                        CAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGT                                  >  1:33410/1‑151                                                                                                               ACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTC                           <  1:149895/151‑1                                                                                                                CCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTC                           >  2:498999/1‑150                                                                                                                  ATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTC                           >  2:291332/1‑148                                                                                                                  ATCATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTC                           >  1:10661/1‑148                                                                                                                     ATACACTAAATCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAAT                     <  2:115107/151‑1                                                                                                                               TCAGTAAGTTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAA           <  1:137532/151‑1                                                                                                                                       TTGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGG    <  1:397380/150‑1                                                                                                                                        TGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCG  <  1:209887/151‑1                                                                                                                                        TGGCAGCATCACCTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCG  <  2:8231/151‑1                                                                                                                                                                                                                                                                                                GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1979123‑1979270 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCG  >  NC_000913/2492323‑2492460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.