Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,966,174	IS1 (–) +9 bp	intergenic (+84/‑235)	trxA → / → rho	thioredoxin 1/transcription termination factor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3583428 =	NA (NA)	36 (1.040)	29/296	0.1	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	3966174 =	0 (0.000)					intergenic (+84/‑243)	trxA/rho	thioredoxin 1/transcription termination factor
*	?	NC_000913	= 3584195	NA (NA)	44 (1.270)	32/296	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 3966182	0 (0.000)					intergenic (+92/‑235)	trxA/rho	thioredoxin 1/transcription termination factor

AATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3584048‑3584195 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAGTGCACCCAC  <  NC_000913/3966182‑3966034                                                                                                                                                                                                                                                                                                           AATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAG                                                                                                                                                     <  1:450801/150‑1        CGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTT                                                                                                                                             <  2:493458/151‑1          GCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAA                                                                                                                                           >  1:355455/1‑151               ACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGA                                                                                                                                      >  1:21443/1‑151               ACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGCGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGA                                                                                                                                      >  2:197507/1‑151                   GAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGT                                                                                                                                   >  2:476952/1‑150                               GCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTA                                                                                                                      >  2:337101/1‑151                                CACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAG                                                                                                                     >  1:348902/1‑151                                CACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTT                                                                                                                       <  2:475819/149‑1                                 ACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGC                                                                                                                    <  1:304352/151‑1                                  CCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCA                                                                                                                   >  2:279203/1‑151                                       CACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACT                                                                                                              <  1:451244/151‑1                                       CACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACT                                                                                                              <  1:33356/151‑1                                       CACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGAC                                                                                                               <  1:382787/150‑1                                                                      AAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCCCGCCGGGCGTCCAGTTTTTAGCGACGGGG                                                                               <  1:356875/151‑1                                                                             GTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGA                                                                        >  1:259687/1‑151                                                                                GAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACA                                                                     >  2:357778/1‑151                                                                                   CTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACAT                                                                    <  1:19936/149‑1                                                                                     GCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAA                                                                >  2:418706/1‑151                                                                                             AAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTA                                                        <  2:551028/151‑1                                                                                                 CATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGC                                                     >  1:52449/1‑150                                                                                                      GGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTT                                               >  1:526533/1‑151                                                                                                          TTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCG                                           <  1:470773/151‑1                                                                                                           TATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGT                                          >  1:114720/1‑151                                                                                                           TATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGT                                          >  1:52/1‑151                                                                                                           TATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGT                                          >  1:359303/1‑151                                                                                                            ATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGT                                          >  1:357918/1‑150                                                                                                                   CATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAAC                                  <  2:149614/151‑1                                                                                                                    ATAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAA                                   >  2:432299/1‑149                                                                                                                     TAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTC                                <  1:80399/151‑1                                                                                                                     TAAAACACTATCAATAAGTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACT                                 >  1:164440/1‑150                                                                                                                                      GTTGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAG               >  1:224174/1‑151                                                                                                                                        tgtAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTGAGTTAGCATGACTCACGCCGTGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCATTAGAC             <  1:143166/148‑1                                                                                                                                        TGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGAC             <  2:226509/151‑1                                                                                                                                        TGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGAC             <  2:177410/151‑1                                                                                                                                        TGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGAC             <  1:521372/151‑1                                                                                                                                        TGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGAC             <  1:337167/151‑1                                                                                                                                        TGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGAC             <  1:182807/151‑1                                                                                                                                        TGGAGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGAC             <  1:106721/151‑1                                                                                                                                           AGTCATTACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAG           <  1:306545/150‑1                                                                                                                                                 TACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAGTGCACC     >  1:47959/1‑150                                                                                                                                                 TACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAGTGCACC     >  2:531463/1‑150                                                                                                                                                  ACCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAGTGCACCCA   <  1:116800/151‑1                                                                                                                                                   CCAGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAGTGCACCCAC  <  2:9937/151‑1                                                                                                                                                                                                                                                                                                           AATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3584048‑3584195 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGTATGTTTAATACGAAGTCAACACTAAGTTAGCATGACTCACGCCGGGCGTCCAGTTTTTAGCGACGGGGCACCCGAACATGAAATTCCCTTACGCCAGGTTAGCGTCGAGGAACTCTTTCAACTGACCTTTAGACAGTGCACCCAC  <  NC_000913/3966182‑3966034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.