Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,274	C→A	G19V (GGC→GTC)	tufA ←	translation elongation factor EF‑Tu 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,274	0	C	A	100.0%	201.6 / NA	53	G19V (GGC→GTC)	tufA	translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/28);  total (25/28)

CGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCA  >  NC_000913/3471128‑3471418                                                                                                                                                   |                                                                                                                                                 cGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGAt                                                                                                                                              >  2:164914/1‑151 (MQ=255) cGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGAt                                                                                                                                              <  2:387395/151‑1 (MQ=255) cGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGAt                                                                                                                                              <  2:423300/151‑1 (MQ=255) cGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGAt                                                                                                                                              >  1:305350/1‑151 (MQ=255)       aaGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTAc                                                                                                                                         <  1:115363/150‑1 (MQ=255)          tgtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGGCGAAGGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCa                                                                                                                                       <  1:29031/149‑1 (MQ=255)              gATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTa                                                                                                                                 >  1:209336/1‑151 (MQ=255)                tGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTaa                                                                                                                                >  1:537956/1‑150 (MQ=255)                  gTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACg                                                                                                                              <  2:478439/150‑1 (MQ=255)                  gTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGt                                                                                                                             >  2:174738/1‑151 (MQ=255)                       aCCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGt                                                                                                                        >  2:340038/1‑151 (MQ=255)                                                ttATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAg                                                                                               >  2:211276/1‑151 (MQ=255)                                                ttATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAg                                                                                               >  1:312832/1‑151 (MQ=255)                                                ttATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAg                                                                                               >  2:428055/1‑151 (MQ=255)                                                            tCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTatat                                                                                   <  1:484576/151‑1 (MQ=255)                                                            tCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTatat                                                                                   <  2:484335/151‑1 (MQ=255)                                                               aaTGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTc                                                                                 <  2:24354/150‑1 (MQ=255)                                                                   cgcGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTAc                                                                            <  1:230629/149‑1 (MQ=255)                                                                       agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                         <  2:290867/150‑1 (MQ=255)                                                                       agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                         <  1:536368/150‑1 (MQ=255)                                                                       agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                         <  1:393206/150‑1 (MQ=255)                                                                         cagcaCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAg                                                                       <  1:398798/150‑1 (MQ=255)                                                                         cagcaCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAg                                                                       <  2:356847/150‑1 (MQ=255)                                                                            caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                    <  1:494099/150‑1 (MQ=255)                                                                            caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                    <  2:261517/150‑1 (MQ=255)                                                                             aCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTGCCAACGTTAGCGTGCTGTTTTGTACGTTCAAATGTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                    <  1:288509/149‑1 (MQ=255)                                                                                gccgTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCtctc                                                                >  2:274645/1‑150 (MQ=255)                                                                                 ccgTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTcc                                                               >  1:43679/1‑150 (MQ=255)                                                                                       gTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                          <  2:312562/149‑1 (MQ=255)                                                                                        ttttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                       <  1:352206/151‑1 (MQ=255)                                                                                          ttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                     <  1:120805/151‑1 (MQ=255)                                                                                            aGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                   >  1:441736/1‑151 (MQ=255)                                                                                              ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                   <  2:479123/149‑1 (MQ=255)                                                                                                 gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                               >  2:332504/1‑150 (MQ=255)                                                                                                 gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                               >  2:227973/1‑150 (MQ=255)                                                                                                 gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                               >  2:119153/1‑150 (MQ=255)                                                                                                       tggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACt                                        <  1:393600/151‑1 (MQ=255)                                                                                                           gATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTgg                                    >  1:206241/1‑151 (MQ=255)                                                                                                             ttGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTa                                  <  1:391856/151‑1 (MQ=255)                                                                                                                   cGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                             <  2:56704/150‑1 (MQ=255)                                                                                                                     gTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                           <  2:393491/150‑1 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                     >  2:236051/1‑120 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                     <  1:236047/120‑1 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                      >  2:169328/1‑151 (MQ=255)                                                                                                                            tagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                     <  2:114296/149‑1 (MQ=255)                                                                                                                            tagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                   >  1:203496/1‑151 (MQ=255)                                                                                                                              gtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                 >  2:452602/1‑151 (MQ=255)                                                                                                                              gtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                 >  1:335809/1‑151 (MQ=255)                                                                                                                              gtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                 >  1:280073/1‑151 (MQ=255)                                                                                                                               ttttACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                 <  1:509722/150‑1 (MQ=255)                                                                                                                                        ggTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg       >  1:535215/1‑151 (MQ=255)                                                                                                                                           cAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCtt    >  2:446113/1‑151 (MQ=255)                                                                                                                                             aCGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa  >  2:386076/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCA  >  NC_000913/3471128‑3471418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑