Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,818,103	A→T	V133E (GTA→GAA)	chbF ←	phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,818,103	0	A	T	100.0%	91.9 / NA	24	V133E (GTA→GAA)	chbF	phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (11/13);  total (11/13)

CATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAA  >  NC_000913/1817961‑1818250                                                                                                                                               |                                                                                                                                                    cATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCa                                                                                                                                             >  1:362665/1‑151 (MQ=255)    cTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCaaaa                                                                                                                                          >  1:104524/1‑151 (MQ=255)                  gAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGt                                                                                                                            >  1:480350/1‑151 (MQ=255)                    atatTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTAc                                                                                                                          <  2:239719/151‑1 (MQ=255)                                ccGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaa                                                                                                              >  1:373474/1‑151 (MQ=255)                                ccGATAAAGCGTTTAAATCCGGTATAACGATAAACGGCTTCAGTGACCATCCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGTTACGCAGACCTATaa                                                                                                              <  1:488677/151‑1 (MQ=255)                                   aTAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaaac                                                                                                            <  2:362673/150‑1 (MQ=255)                                   aTAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaaac                                                                                                            <  1:57120/150‑1 (MQ=255)                                   aTAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaaac                                                                                                            <  1:487639/150‑1 (MQ=255)                                                     gTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTgg                                                                                         >  1:338407/1‑151 (MQ=255)                                                          aCGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTc                                                                                     >  1:187957/1‑150 (MQ=255)                                                                    gCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGAt                                                                          >  2:138871/1‑151 (MQ=255)                                                                           tGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCa                                                                     <  2:187961/149‑1 (MQ=255)                                                                           tGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCa                                                                     <  2:321245/149‑1 (MQ=255)                                                                             aCCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGa                                                                  >  2:117256/1‑150 (MQ=255)                                                                                         gggTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATa                                                      >  2:435961/1‑150 (MQ=255)                                                                                                  aaGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATcc                                             <  2:22304/150‑1 (MQ=255)                                                                                                    gTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTAGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGt                                          >  1:158434/1‑151 (MQ=255)                                                                                                           aCCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCAcgcg                                   <  2:351561/151‑1 (MQ=255)                                                                                                                                         tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag     <  1:293936/151‑1 (MQ=255)                                                                                                                                         tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag     <  2:166895/151‑1 (MQ=255)                                                                                                                                         tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag     >  2:236711/1‑151 (MQ=255)                                                                                                                                         tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag     <  1:114163/151‑1 (MQ=255)                                                                                                                                          cTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtagt    <  1:423949/151‑1 (MQ=255)                                                                                                                                            ttttCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTaa  <  2:404491/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    CATCTTCATGCCGATCGGAATATTACACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAA  >  NC_000913/1817961‑1818250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑