Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_pgi	3,578,650	A→C	L26* (TTA→TGA)	gntR ←	d‑gluconate inducible gluconate regulon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_pgi	3,578,650	0	A	C	100.0%	214.1 / NA	76	L26* (TTA→TGA)	gntR	d‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (38/38);  total (38/38)

AGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAA  >  NC_000913_3_hsa_pgi/3578509‑3578795                                                                                                                                              |                                                                                                                                                  agagaAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGTATATCGGGCGCACGATTGGGAATATAGCCCAGTTAATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAACCATGCTCAGGGTTGCGTcaaaaaag                                                                                                                                            >  2:379423/1‑147 (MQ=255)  gagcAGGGATCAGGACGCGAAGCGCCCGGCGGGTGGCGGTAGAGAGGATATCGGGCGCTCGAGTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACgg                                                                                                                                           <  2:259507/149‑1 (MQ=255)   agaAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGc                                                                                                                                          <  2:1081581/149‑1 (MQ=255)     aaGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGcgg                                                                                                                                        <  2:1146910/149‑3 (MQ=255)     aaGCTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTg                                                                                                                                        <  1:417756/149‑1 (MQ=255)      aGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGa                                                                                                                                       <  1:115277/149‑1 (MQ=255)        gTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCTAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGAcc                                                                                                                                     >  2:1059753/1‑149 (MQ=255)        gTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGAcc                                                                                                                                     >  1:1180623/1‑149 (MQ=255)        gTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGAcc                                                                                                                                     >  2:303242/1‑149 (MQ=255)          aaCAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGt                                                                                                                                   >  2:947958/1‑149 (MQ=255)            cAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATAACGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGGCa                                                                                                                                 >  1:562110/1‑149 (MQ=255)              ggACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCAtt                                                                                                                               >  1:181280/1‑149 (MQ=255)                aCGCGAATCGCCCGGCTGGTGGAGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAAGCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGGTGCGTCAAAAACGGCTCACCGTCAtttt                                                                                                                             <  2:89011/149‑1 (MQ=255)                aCGCCAATCGCCCGGCTGGAGGCGTTAGAGAGGATATCGGTCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCTAAAACGGCTGACCGTCAtttt                                                                                                                             <  1:1113968/149‑1 (MQ=255)                  gCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTgg                                                                                                                           <  1:633728/149‑1 (MQ=255)                      aTCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCAc                                                                                                                       <  2:517562/149‑1 (MQ=255)                       tCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCCAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACg                                                                                                                      <  1:841884/149‑1 (MQ=255)                        cGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGGCAAAAACGGCTGACCGTCATTTTGGTCACGc                                                                                                                     <  1:673110/149‑1 (MQ=255)                         gCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAACCGGCTGACCGTCATTTTGGTAACGcc                                                                                                                    >  2:1176099/1‑149 (MQ=255)                         gCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGcc                                                                                                                    <  2:1170325/149‑1 (MQ=255)                         gCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGcc                                                                                                                    >  2:1135924/1‑149 (MQ=255)                           ccGGCTGGTGGCGTTAGTGAGGATATCGGGCGCACGATTGGGATTATAGCCCATTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGGTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTa                                                                                                                  <  1:985230/149‑1 (MQ=255)                           ccGGCTGGGGGGGTTAGAGTTGATTTCGGGCCCACGCTTCGGAATATAGCCCTTTTCATCATTAGCCGCGGCAATGTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCTTCAAAAACGGCAGACCGTCATTTTGGTCACGCCTa                                                                                                                  <  1:845893/149‑1 (MQ=255)                           ccGGCGGGTGGCTTTAGAGATGATATCGGGCGCACGTATGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTa                                                                                                                  <  2:1200225/149‑1 (MQ=255)                               cTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACg                                                                                                              <  2:911884/149‑1 (MQ=255)                                  gtggCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACTGCTGACCGTCATTTTGGTCACGCCTACACGGTc                                                                                                           >  2:1163185/1‑149 (MQ=255)                                   tggCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCa                                                                                                          <  1:78909/149‑1 (MQ=255)                                   tggCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGGTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCa                                                                                                          >  1:178333/1‑149 (MQ=255)                                   gggCGTTAGAGAGGATATCGTGCGCACGCTTGGGAATATATCCCAGTTCATCATGAGCCGCGGCAATCTTGCCGCGTAGAGCGACTGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACGCGGTCa                                                                                                          <  2:1036401/148‑1 (MQ=255)                                       gTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCca                                                                                                      >  1:212263/1‑149 (MQ=255)                                        ttAGAGAGGTTATCGGGCGAACGATTGGGAATATAGCCAACTTCATCAAGAGCCGCGGCAAACTTGCAGCGTAGAGCGAAGGAAACCTGGTACGGGTTGCGACAAAAACCGCTGACCGTCATTTTGGTCACGCCTCCACGGGCAGCcac                                                                                                     >  2:210319/1‑149 (MQ=255)                                           gagagGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATc                                                                                                  >  2:539415/1‑149 (MQ=255)                                             gagGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGAAGGAAACCTGATCCGGGTTGCGTCAAAAAAGGGTGACATTAATTTTGGTATCGACTTCACGGTCATACACATACt                                                                                                >  2:741901/1‑149 (MQ=255)                                              agGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTg                                                                                               <  2:30946/149‑1 (MQ=255)                                                   atCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTa                                                                                          >  1:893953/1‑149 (MQ=255)                                                        gcgcACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGACGCGTAGAGCGACTGAAAACTGCTCAGGTATCCGTCAAAAACGACTGAAAGTCATAAAGGTCACGCCAAAACCATCTCCCACATACTAAAgatcagga                                                                                     >  1:864661/1‑142 (MQ=255)                                                          gcACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCAGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtct                                                                                   <  1:455223/149‑1 (MQ=255)                                                            aCGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGCCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtcttt                                                                                 >  1:683136/1‑149 (MQ=255)                                                             cGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGCAACCGGCTCCGGGTTGCGTCAAAAACGGCTGCCCGTCCTTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGgctttt                                                                                >  1:228903/1‑149 (MQ=255)                                                             cGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTAACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtcggtt                                                                                >  2:541651/1‑145 (MQ=255)                                                                ttGGGAATATAGCCCAGTTCATCAAGAGCCGCGGGAATCATGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGtcttttctt                                                                             <  2:192045/149‑1 (MQ=255)                                                                   ggAATATAGCCCAGTTCATCACGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAACCGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCttttt                                                                          <  2:822864/149‑1 (MQ=255)                                                                    gAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTc                                                                         >  1:1121388/1‑149 (MQ=255)                                                                    gAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTc                                                                         <  1:740469/149‑1 (MQ=255)                                                                     aaTATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGGCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCa                                                                        <  1:1123102/149‑1 (MQ=255)                                                                       tataGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATc                                                                      >  1:556683/1‑149 (MQ=255)                                                                       tataGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATc                                                                      >  1:941304/1‑149 (MQ=255)                                                                           gCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTcc                                                                  >  1:642117/1‑149 (MQ=255)                                                                            cccAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACCTCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCt                                                                 >  2:20437/1‑149 (MQ=255)                                                                                gTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCAAGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAg                                                             >  1:1074028/1‑149 (MQ=255)                                                                                       aaGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGCCCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACaaa                                                      >  2:161064/1‑149 (MQ=255)                                                                                         tctacgcgGCAAGATTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGCCCGTCATGTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCGTGTCTTTTTCATCGTCCTGAAGGTACATAag                                                    >  1:336477/5‑149 (MQ=255)                                                                                         gagCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTTCGGGTCTTTTCTTTTTCGTCGTCCTGAAGGTACAAAag                                                    >  2:1133316/1‑149 (MQ=255)                                                                                          agCCGCGGCAATCTTGCCGCGGAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAAGGGCTGACCGTCATTTTGGACACGCCTTCACGGTCAGCCACATCCTGAAGTACGGGTCTTGTCTTTTTCATCGTCCTGAAGGTACAAAaga                                                   <  1:114448/149‑1 (MQ=255)                                                                                           tacgcgGCAAGATTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCCCGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAagag                                                  >  1:480295/3‑149 (MQ=255)                                                                                           tacgcgGCAAGATTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACCTCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAagag                                                  >  2:171861/3‑149 (MQ=255)                                                                                              gcgGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGTGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGagata                                               <  1:238839/149‑1 (MQ=255)                                                                                              gcgGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGAACGTCATTATGGTCACGCCTACAAGGTCAGCCACATCCTGAAGTACGGGTCTTTTATTTTTCATCGTCCTGAAGGTACAAAAGagatt                                               >  1:754254/1‑148 (MQ=255)                                                                                                ggCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGagataga                                             >  2:1235773/1‑149 (MQ=255)                                                                                                       ttGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTAGAAAAGAGATAGATTCTCtt                                      <  1:379423/149‑1 (MQ=255)                                                                                                        tGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGTTCAGGCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTa                                     <  2:603999/149‑1 (MQ=255)                                                                                                        tGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTa                                     <  2:212263/149‑1 (MQ=255)                                                                                                         tccGCGTAGAGCTACGGAAACATGCTCCGGGTTGCGTCAAATACGGCTGACCGTCATTTTGGTCAGGCCTACACGGTCAGCCACATCCTTAAGTACGGTTCTTTTCTTTTTCATCGTCCTGAATGTACAAAAGAGATAGATTCTCTTAg                                    <  2:562110/148‑1 (MQ=255)                                                                                                            gcgTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGttt                                 <  1:1135924/149‑1 (MQ=255)                                                                                                             cgTAGAGCGACGGAAACCTGCTCCGGGTTGCGTCAACAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCCTCGTCCTGAAGGTACAAAAGGGATAGATTCTCTTAGTTTa                                >  2:662040/1‑149 (MQ=255)                                                                                                              gTAGAGCGACGGAAACCTGCTCGGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGTGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTaa                               <  2:228627/149‑1 (MQ=255)                                                                                                                 gagCGACGGAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAacac                            >  1:972744/1‑149 (MQ=255)                                                                                                                       cGGAAACCTGCTCCGGGGTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGCCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGacac                      <  2:128172/149‑1 (MQ=255)                                                                                                                        ggAAACCTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCCCGCCTACACGGTCAGCCACATCCTGAAGTACGGCTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTCAACACGGacacc                     >  2:753891/1‑148 (MQ=255)                                                                                                                          aaaCCTGCTCCGGGTTGCATCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATc                   >  2:1221231/1‑149 (MQ=255)                                                                                                                             ccTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCAATTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGGCTTTGCTTTTACATCGTCCTGAAGGAACAAAAGAGATAGATGCTCTTAGATTAACACGGCCCCATCAGa                >  1:677562/1‑149 (MQ=255)                                                                                                                              cTGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGaa               <  1:1005893/149‑1 (MQ=255)                                                                                                                               tGCTCCGGGTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAAc              <  1:947958/149‑1 (MQ=255)                                                                                                                                       gTTGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCgg      <  1:539415/149‑1 (MQ=255)                                                                                                                                        ttGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCggg     >  1:456231/1‑149 (MQ=255)                                                                                                                                         tGCGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCgggg    <  1:133508/149‑1 (MQ=255)                                                                                                                                           cGTCAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGaa  <  1:1085506/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  AGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAACCTGCTCCGGGTTGCGTAAAAAACGGCTGACCGTCATTTTGGTCACGCCTACACGGTCAGCCACATCCTGAAGTACGGGTCTTTTCTTTTTCATCGTCCTGAAGGTACAAAAGAGATAGATTCTCTTAGTTTAACACGGACACATCAGAACCTTCCCGGGGAA  >  NC_000913_3_hsa_pgi/3578509‑3578795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑