Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,840 +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42338400 (0.000)67 (1.140)
+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA
53/188 0.1 100% intergenic (‑607/‑500) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233841 = NA (NA)intergenic (‑608/‑499) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 107 total aligned reads displayed.

GGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAA  >  NC_000913_3_KO_pgi/4233706‑4233990
                                                                                                                                       |                                                                                                                                                     
cactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATg                                                                                                                                         <  1:887049‑M2/15‑1 (MQ=255)
 actacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                        <  2:223216‑M2/16‑1 (MQ=255)
  ctacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTa                                                                                                                                       >  4:532344‑M2/134‑150 (MQ=255)
   tacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAg                                                                                                                                      >  1:849785‑M2/133‑150 (MQ=255)
   tacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAg                                                                                                                                      >  2:854096‑M2/133‑150 (MQ=255)
      ggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGcc                                                                                                                                   >  3:297119‑M2/130‑150 (MQ=255)
          gtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatcccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGAt                                                                                                                               <  1:444463‑M2/25‑1 (MQ=255)
             gctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                            <  1:841586‑M2/28‑1 (MQ=255)
               tggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATAGGTAGGCCGGATAAGGc                                                                                                                          <  2:866904‑M2/30‑1 (MQ=255)
                  agctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                                       <  1:911101‑M2/33‑1 (MQ=255)
                       cttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                  <  4:111466‑M2/38‑1 (MQ=255)
                        ttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                 >  1:550126‑M2/112‑150 (MQ=255)
                        ttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgaggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                 >  1:901414‑M2/112‑150 (MQ=255)
                           gaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                              >  2:377619‑M2/109‑150 (MQ=255)
                              gttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                           >  1:138263‑M2/106‑150 (MQ=255)
                               ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                          <  1:460313‑M2/46‑1 (MQ=255)
                               ttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                          >  1:632221‑M2/105‑150 (MQ=255)
                                  ctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatc                                                                                                                                                     >  3:43158‑M2/102‑104 (MQ=255)
                                  ctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatc                                                                                                                                                     <  4:43158‑M2/3‑1 (MQ=255)
                                   tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  2:618816‑M2/101‑150 (MQ=255)
                                   tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  1:200956‑M2/101‑150 (MQ=255)
                                     tactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                                    >  1:827974‑M2/99‑150 (MQ=255)
                                      actttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATAATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg                                                                                                   >  3:558880‑M2/98‑150 (MQ=255)
                                       ctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                                  <  3:584689‑M2/54‑1 (MQ=255)
                                        tttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctatacagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                                 <  1:318879‑M2/55‑1 (MQ=255)
                                        tttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaggcgtggcgcggttaATCATCGTCGCTATGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAACCGAt                                                                                                 <  4:227385‑M2/55‑1 (MQ=255)
                                        tttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                                 >  2:753943‑M2/96‑150 (MQ=255)
                                            tagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCt                                                                                             >  1:36910‑M2/92‑150 (MQ=255)
                                             agagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                                            >  3:83704‑M2/91‑150 (MQ=255)
                                             agagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                                            >  4:124315‑M2/91‑150 (MQ=255)
                                              gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                           >  1:196316‑M2/90‑150 (MQ=255)
                                                gaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                                         <  2:179580‑M2/63‑1 (MQ=255)
                                                gaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                                         <  4:75707‑M2/63‑1 (MQ=255)
                                                       aacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCAGCATCCGGCAACAGATGCCTGATGCGAcgcg                                                                                  <  2:67366‑M2/70‑1 (MQ=255)
                                                         cttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                                >  3:421946‑M2/79‑150 (MQ=255)
                                                          ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               <  4:314950‑M2/73‑1 (MQ=255)
                                                          ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               >  2:310951‑M2/78‑150 (MQ=255)
                                                           tcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcg                                                                              >  1:736516‑M2/77‑150 (MQ=255)
                                                            cgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCTTGCACGCCGCATCCGGCAACCGATTCCTGATGAGACGAGGTcgc                                                                             <  2:116247‑M2/75‑1 (MQ=255)
                                                              aactgcaggtcgacggatccccggaatgctcgaccaatggtgtgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                           <  2:93264‑M2/77‑1 (MQ=255)
                                                              aactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                           <  3:233217‑M2/77‑1 (MQ=255)
                                                                 tgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                                        >  3:511968‑M2/71‑150 (MQ=255)
                                                                  gcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                       <  1:101830‑M2/81‑1 (MQ=255)
                                                                   caggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                                      <  3:488692‑M2/82‑1 (MQ=255)
                                                                    aggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                     <  2:427465‑M2/83‑1 (MQ=255)
                                                                     ggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                                    <  3:368137‑M2/84‑1 (MQ=255)
                                                                      gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                                   >  1:184839‑M2/66‑150 (MQ=255)
                                                                       tcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg                                                                  >  2:405480‑M2/65‑150 (MQ=255)
                                                                        cgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                 <  2:736516‑M2/87‑1 (MQ=255)
                                                                        cgacggatccccggaatgctcgaccaagggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                                 <  3:124315‑M2/87‑1 (MQ=255)
                                                                          acggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCAATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCt                                                               >  1:612063‑M2/62‑150 (MQ=255)
                                                                            ggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                             <  3:388865‑M2/91‑1 (MQ=255)
                                                                               tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                          >  3:109873‑M2/57‑150 (MQ=255)
                                                                               tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaACCATCGTCGATATGTAGGCCCGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                          >  2:450270‑M2/57‑150 (MQ=255)
                                                                                 cccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATaa                                                                                                                             >  2:311674‑M2/55‑81 (MQ=255)
                                                                                 cccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATaa                                                                                                                             <  1:311674‑M2/27‑1 (MQ=255)
                                                                                 cccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                         >  2:282321‑M2/55‑149 (MQ=255)
                                                                                 cccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                         <  1:282321‑M2/95‑1 (MQ=255)
                                                                                 cccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                                        >  1:253059‑M2/55‑150 (MQ=255)
                                                                                  ccggaatgctcgaccaatggtctgattaaccgctataaagcgttgcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                       >  1:834601‑M2/54‑150 (MQ=255)
                                                                                   cggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                                      <  2:827974‑M2/98‑1 (MQ=255)
                                                                                    ggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAt                                                     <  2:550126‑M2/99‑1 (MQ=255)
                                                                                         gctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                  <  2:732837‑M2/38‑1 (MQ=255)
                                                                                         gctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                  >  1:732837‑M2/47‑84 (MQ=255)
                                                                                          ctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                               <  2:184839‑M2/105‑1 (MQ=255)
                                                                                           tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCgatt                                              >  1:549264‑M2/45‑149 (MQ=255)
                                                                                           tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                              >  1:892076‑M2/45‑150 (MQ=255)
                                                                                           tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATAATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                              >  3:236587‑M2/45‑150 (MQ=255)
                                                                                            cgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                             <  1:320432‑M2/107‑1 (MQ=255)
                                                                                              accaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                           >  1:637633‑M2/42‑150 (MQ=255)
                                                                                                caatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                         <  2:849785‑M2/111‑1 (MQ=255)
                                                                                                     gtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGt                                    >  4:417739‑M2/35‑150 (MQ=255)
                                                                                                       ctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                               <  2:751963‑M2/105‑1 (MQ=255)
                                                                                                       ctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                               >  1:751963‑M2/33‑137 (MQ=255)
                                                                                                       ctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAt                                  >  4:51261‑M2/33‑150 (MQ=255)
                                                                                                         gattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                                >  1:920063‑M2/31‑150 (MQ=255)
                                                                                                         gattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                                >  1:920179‑M2/31‑150 (MQ=255)
                                                                                                          attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                               <  3:278995‑M2/121‑1 (MQ=255)
                                                                                                             aaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa                            <  1:310951‑M2/124‑1 (MQ=255)
                                                                                                              accgctataaagcgtggcgcggttaATCATAGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                           >  1:150196‑M2/26‑150 (MQ=255)
                                                                                                               ccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                   <  4:451851‑M2/101‑1 (MQ=255)
                                                                                                               ccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                   >  3:451851‑M2/25‑125 (MQ=255)
                                                                                                                cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCCTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                         >  1:573776‑M2/24‑150 (MQ=255)
                                                                                                                  ctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa                       <  4:260683‑M2/129‑1 (MQ=255)
                                                                                                                   tataaagcgtggcgcggttaATCATCGTCGAGATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt                      <  2:184684‑M2/130‑1 (MQ=255)
                                                                                                                   tataaagcgtggcgcggttaATCATCGCCGATACGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCACGCCTACAGGTCGATGCCGATATCTACATCGTATCCGGCAATTAAt                      >  2:315737‑M2/21‑150 (MQ=255)
                                                                                                                    ataaagcgtggcgcgggtaATCAGCGTCGATAGGTAGGCCGGATAAGTCGTTCACGCCGCTTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACCTCGTATTCGGCAATTAATa                     <  4:428350‑M2/131‑1 (MQ=255)
                                                                                                                     taaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                    >  1:603637‑M2/19‑150 (MQ=255)
                                                                                                                       aagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt                  <  1:71850‑M2/134‑1 (MQ=255)
                                                                                                                        agcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa                 <  2:308048‑M2/135‑1 (MQ=255)
                                                                                                                           gtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTCATCGTATTCGGCAATTAATACATAGCAc             >  4:34383‑M2/13‑150 (MQ=255)
                                                                                                                              gcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACga           <  2:637633‑M2/141‑1 (MQ=255)
                                                                                                                                 cggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattg        >  3:277759‑M2/7‑150 (MQ=255)
                                                                                                                                   gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat      >  3:517728‑M2/5‑150 (MQ=255)
                                                                                                                                    ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                                <  1:746558‑M2/88‑1 (MQ=255)
                                                                                                                                    ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                                >  2:746558‑M2/4‑91 (MQ=255)
                                                                                                                                    ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                           >  1:517971‑M2/4‑112 (MQ=255)
                                                                                                                                    ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                           <  2:517971‑M2/109‑1 (MQ=255)
                                                                                                                                      aATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTaa   <  4:511968‑M2/149‑1 (MQ=255)
                                                                                                                                       atcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGACGCGACGCGGTCGCGTCGTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTaaa  <  1:788345/150‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                     
GGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAA  >  NC_000913_3_KO_pgi/4233706‑4233990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.