New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi 3221823 =24 (0.820)5 (0.180) 4/266 3.5 15.2% coding (358/984 nt) ebgR transcriptional repressor
?NC_000913_3_pae_pgi 3221880 = 33 (1.190)coding (415/984 nt) ebgR transcriptional repressor
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGA  >  NC_000913_3_pae_pgi/3221681‑3221965
                                                                                                                                              |                                                                                                                                              
gcagGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCg                                                                                                                                          >  1:333062/1‑149 (MQ=255)
    gAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCCCCGGTATTTTAATTGTCGGCaa                                                                                                                                      >  1:211515/1‑149 (MQ=255)
        tGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAAccc                                                                                                                                  >  1:228704/1‑149 (MQ=255)
             aTCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGACATTATGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCAcgcc                                                                                                                             >  1:360283/1‑149 (MQ=255)
                aaCGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCAcgaaggc                                                                                                                          >  1:247212/1‑144 (MQ=255)
                                              ggCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGa                                                                                            >  2:471740/1‑149 (MQ=255)
                                                        ccccGTGCGAAAACCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGTTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGt                                                                                  <  1:432802/149‑1 (MQ=255)
                                                         ccAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCACCGGCCTACTTGCGCGCCGCTGCCAGCGCGTTGACCGACAAGATCTGtt                                                                                 >  1:227647/1‑149 (MQ=255)
                                                                    aGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAGACCCACGCCCGCCCTGCGCGCCGCTGCCAACGCGTTGACCGACAATATCTGTTTTATCGACttt                                                                      >  2:416054/1‑149 (MQ=255)
                                                                                     aCCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATAGACTTTCACGAACCCGGCAGCgg                                                     >  1:321532/1‑149 (MQ=255)
                                                                                       cAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGtt                                                   >  2:350252/1‑149 (MQ=255)
                                                                                        aaCGGTTATGAACACAGCGGCTTATCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTa                                                  <  2:170502/149‑1 (MQ=255)
                                                                                               aTGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCAGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCg                                           >  2:56510/1‑149 (MQ=255)
                                                                                                       aGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCAcgcc                                                                                                                             <  1:11236/59‑1 (MQ=255)
                                                                                                       aGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCAcgcc                                                                                                                             >  2:11236/1‑59 (MQ=255)
                                                                                                          ggCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGGTACGCTGCGGTGGCTATCGa                                >  2:359998/1‑149 (MQ=255)
                                                                                                            cTTACCAGACATTAACAACGTCACCGGTCTTTTAATTGTCGGCAACCCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTCGAACGATCGTATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATc                              <  1:305442/149‑1 (MQ=255)
                                                                                                             ttACCAGACATTAAAATCGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCt                             <  2:227647/149‑1 (MQ=255)
                                                                                                                   gACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGCATATCGATCTGGCACg                       >  2:333359/1‑149 (MQ=255)
                                                                                                                        tAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCa                  <  2:8409/149‑1 (MQ=255)
                                                                                                                          aaaaCCTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCCCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGt                <  1:145568/149‑1 (MQ=255)
                                                                                                                             aCGTCCCCGGTATGTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTaaa             <  2:48464/149‑1 (MQ=255)
                                                                                                                             aCGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCTGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTaaa             <  2:333062/149‑1 (MQ=255)
                                                                                                                                        aTTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGa  <  2:228704/149‑1 (MQ=255)
                                                                                                                                              aTTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCa                                                                    <  2:168913/77‑1 (MQ=255)
                                                                                                                                              aTTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCa                                                                    >  1:168913/1‑77 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GCAGGAGCTGGAGATCAACGATCCTTACTATCTGGCGATCCGCCACGGCATTGAAACCCAGTGCGAAAAGCTGGGCATCGAGCTCACCAACTGTTATGAACACAGCGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCGCGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGCATCAGTAAAGAAATCATCGA  >  NC_000913_3_pae_pgi/3221681‑3221965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.