| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_pae_pgi | 951204 = | 31 (1.150) | 5 (0.190) | 5/278 | 2.9 | 13.4% | intergenic (‑124/+68) | pflA/pflB | pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1 |
| ? | NC_000913_3_pae_pgi | 951236 = | 34 (1.280) | intergenic (‑156/+36) | pflA/pflB | pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase/formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1 | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
GACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTG > NC_000913_3_pae_pgi/951065‑951345 | gACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGcc > 1:167287/1‑149 (MQ=255) aCCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAGAAAGccc > 2:106495/1‑149 (MQ=255) gACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTaaaaaa > 2:292743/1‑134 (MQ=255) gACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTaaaaaa < 1:292743/134‑1 (MQ=255) ttGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTACGTGGAGc < 1:337660/149‑1 (MQ=255) tgtTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGTAGCTCGAAGGCTACGTCGATTCTTTTTTTGCAGTCACCTTAAAGTATAGAGAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTAt < 1:232439/149‑1 (MQ=255) gctaggcagtacgcttcaactcgctcacgaaagaacagcagaaggacgttattactcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTg > 1:134435‑M1/117‑149 (MQ=255) tctcGAGATGTGGCCCATCTTAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTa < 2:377507/149‑1 (MQ=255) tcCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAACAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTTTAcc > 1:208145/1‑148 (MQ=255) cAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATGGCCTGTAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCt > 1:110855/1‑149 (MQ=255) cAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCt > 1:372656/1‑149 (MQ=255) ccATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTa < 1:55118/149‑1 (MQ=255) ccGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAATCTGTTTTGGCAGTCACCTGAAAGTATTGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCGGt > 2:13131/1‑149 (MQ=255) taaagaacagcagcaggacgttattactcgtaccttcactcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCa < 1:426458‑M1/61‑1 (MQ=255) ggtTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGGTAGCTGACAAAACAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTAAGATTTCAGTCaa > 2:268193/2‑149 (MQ=255) cAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTAc > 2:198557/1‑149 (MQ=255) aGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACCAACGAGGTTCTCGCGCTCAAAAAAGCCCCCCTTTCGTGAAGCAGTTATTGTACACTTTTTACTGTCCGCTTGCAGTCAAATCTAATTACa > 1:233426/1‑149 (MQ=255) ggCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGa < 1:100080/149‑1 (MQ=255) gcgaCGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAg < 2:218009/146‑1 (MQ=255) ttactcttaccttcaatcaaattatgtaattagatttgactgaaatcgtacagtaaaaagcgtacAAAAAAGGCCCCATTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTg > 2:426458‑M1/66‑149 (MQ=255) cGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCGGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTAAATAAATTGGTTGaa > 1:198157/1‑149 (MQ=255) tCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGaa > 1:38504/1‑142 (MQ=255) tCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGaa < 2:38504/142‑1 (MQ=255) tCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGa > 1:462791/1‑149 (MQ=255) ttttGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGGGAAGGTACGAGtac > 2:68657/1‑148 (MQ=255) tcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacAATAAAGGCCCCACTTTCGTGGa > 2:78044‑M1/50‑72 (MQ=255) tcaatctatgtaattagatttgactgaaatcgtacagtaaaaagcgtacAATAAAGGCCCCACTTTCGTGGa < 1:78044‑M1/23‑1 (MQ=255) aCCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGTTACGCGTAATAACGTCcttc > 2:243413/1‑147 (MQ=255) atagatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCtt < 2:110855/149‑1 (MQ=255) ggacagCTGACAACAACGGTTCTCGCGCTAAAAAAGGCCCCACTTTCGGGGAGCCTTTATTGTACGCATTTTACTGGACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTATGAGTAACAACGTCCTGCTGCTGTTCTTTa < 2:139388/145‑1 (MQ=255) agatagCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTa < 2:409482/149‑1 (MQ=255) agcacaCAAACACGGGTCTGGGGCGAAAAAAAGCCGCACTTTTGTAGTGAACTTAGTGTCCGCTTTTTACAGTACGATTTCAGTCCAATCTAATTACATAGATTGAGTGAAGGGACCAGTAATAACGTCCTGCTGCTGTTCTTTAGTCa < 2:451619/144‑1 (MQ=255) aaaaaaaGTCTCTCGCGCTAAAAAAGGCTCCACTTTCAAGAAGGGCGTATGTTACGCTTTTGTTTGTACGATTTCATTCAAATCTAATTACATCGATTGAGTGAAGGTACGAGTAATATCGTCCTGCTGCTGTTCTTTAGTCAGCGAGt < 2:50325/148‑1 (MQ=255) aaaaaaGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTGTTACTGTACGATTTCAGGCAAATCTAATTACATAGATTGAGTGAAAGTATGAGGGATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGtt < 1:114180/149‑1 (MQ=255) ctcGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACt < 1:92354/149‑1 (MQ=255) tcGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTg < 1:198557/149‑1 (MQ=255) | GACCAATAACTGACATTGCGGTGTTTCTCCAGATGTGGCCCATCTGAGGCCGTGTTGGTGCGCAGCTCGAAGGCTACGTCGAGTCTGTTTTGGCAGTCACCTTAAAGTATAGATAGCTGACAAAAAAGGCTCTCGCGCTAAAAAAGGCCCCACTTTCGTGGAGCCTTTATTGTACGCTTTTTACTGTACGATTTCAGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTG > NC_000913_3_pae_pgi/951065‑951345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |