Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	3,440,173	T→C	E286G (GAG→GGG)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	3,440,173	0	T	C	100.0%	332.3 / NA	94	E286G (GAG→GGG)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/50);  total (44/50)

ACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGAC  >  NC_000913_3_pae_pgi/3440027‑3440317                                                                                                                                                   |                                                                                                                                                 aCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCgg                                                                                                                                               >  1:337913/1‑150 (MQ=255)     gtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACg                                                                                                                                           >  2:1094391/1‑150 (MQ=255)      tgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGc                                                                                                                                          >  3:601983/1‑150 (MQ=255)      tgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGc                                                                                                                                          <  1:437837/150‑1 (MQ=255)        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTg                                                                                                                                        >  1:684436/1‑150 (MQ=255)        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTg                                                                                                                                        >  1:499010/1‑150 (MQ=255)        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTg                                                                                                                                        >  4:119432/1‑150 (MQ=255)        aTCCGGTTACTCGCAAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTg                                                                                                                                        <  1:61748/150‑1 (MQ=255)           cGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTAc                                                                                                                                     <  2:684436/150‑1 (MQ=255)           cGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTAc                                                                                                                                     >  3:667248/1‑150 (MQ=255)            ggTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTAc                                                                                                                                     <  2:1106410/149‑1 (MQ=255)            ggTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTAc                                                                                                                                     >  1:1106410/1‑149 (MQ=255)             gTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCa                                                                                                                                   >  1:1099113/1‑150 (MQ=255)                  tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCAAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCa                                                                                                                              >  4:441387/1‑150 (MQ=255)                    gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCAcc                                                                                                                            >  1:1064218/1‑150 (MQ=255)                    gTCAGCGATGCTTGCCGGTGGCCAGTTTTCAAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCAcc                                                                                                                            <  3:441387/150‑1 (MQ=255)                     tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCg                                                                                                                           <  4:325061/150‑1 (MQ=255)                     tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCg                                                                                                                           >  2:667354/1‑150 (MQ=255)                         cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGata                                                                                                                        <  4:542925/149‑1 (MQ=255)                         cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGata                                                                                                                        >  3:542925/1‑149 (MQ=255)                           aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAg                                                                                                                     <  1:1084333/150‑1 (MQ=255)                            tGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGt                                                                                                                    <  2:538002/150‑1 (MQ=255)                             gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTg                                                                                                                   <  2:504242/150‑1 (MQ=255)                              cTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTgg                                                                                                                  >  2:604630/1‑150 (MQ=255)                               ttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCAcc                                                                                                                            <  3:151380/139‑1 (MQ=255)                               ttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCAcc                                                                                                                            >  4:151380/1‑139 (MQ=255)                               ttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGa                                                                                                                 <  4:667248/150‑1 (MQ=255)                                 gCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATa                                                                                                               >  1:341777/1‑150 (MQ=255)                                   cGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGc                                                                                                             >  3:539209/1‑150 (MQ=255)                                     gTGGCCAGTTTTCCAGGCGCCTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt                                                                                                           <  2:225726/150‑1 (MQ=255)                                      tGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                                                                                                          <  1:667354/150‑1 (MQ=255)                                      tGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                                                                                                          <  1:1094391/150‑1 (MQ=255)                                        gCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCGCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAACGTTAGGCGTTTTAAGGAGCTCACCCCCGGTACGCTGGACCAGATCACCGATATAGTGGATAGCTTCTg                                                                                                        <  1:1081505/150‑1 (MQ=255)                                        gCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                                                                                                        >  2:605911/1‑150 (MQ=255)                                             ttttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCg                                                                                                                                                <  1:848205/105‑1 (MQ=255)                                             ttttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTa                                                                                                   >  3:74094/1‑150 (MQ=255)                                               ttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg                                                                                                 <  1:981136/150‑1 (MQ=255)                                                  cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATc                                                                                                                               <  2:824405/117‑1 (MQ=255)                                                  cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATc                                                                                                                               >  1:824405/1‑117 (MQ=255)                                                   aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAg                                                                                             >  3:65829/1‑150 (MQ=255)                                                    ggCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt                                                                                            <  2:1062911/150‑1 (MQ=255)                                                     gcgcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGtt                                                                                           <  4:74094/150‑1 (MQ=255)                                                       gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg                                                                                         <  2:155254/150‑1 (MQ=255)                                                            cccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                                                                    <  4:601983/150‑1 (MQ=255)                                                             ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                                                                    <  1:796468/149‑1 (MQ=255)                                                             ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                                                                    >  2:796468/1‑149 (MQ=255)                                                             ccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc                                                                                   >  1:348345/1‑150 (MQ=255)                                                              cAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg                                                                                  <  2:889004/150‑1 (MQ=255)                                                               agagACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCgg                                                                                 >  1:111189/1‑150 (MQ=255)                                                                   aCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCg                                                                                                                           <  2:68475/104‑1 (MQ=255)                                                                   aCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGCACCAGATCACCg                                                                                                                           >  1:68475/1‑104 (MQ=255)                                                                    cAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAgg                                                                            <  4:65829/150‑2 (MQ=255)                                                                       tCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCaa                                                                         >  1:342138/1‑150 (MQ=255)                                                                         cACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACa                                                                              >  4:425546/1‑143 (MQ=255)                                                                         cACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACa                                                                              <  3:425546/143‑1 (MQ=255)                                                                          aCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTc                                                                      >  2:301361/1‑150 (MQ=255)                                                                          aCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTc                                                                      >  1:389884/1‑150 (MQ=255)                                                                              gAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGa                                                                  <  2:341777/150‑1 (MQ=255)                                                                               aaGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGAt                                                                 <  2:499010/150‑1 (MQ=255)                                                                                aGCCAGCGCGTCTTTAATCTGAGTAATATATTTTTTACCAAGGTTAGGCGGTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCTGTTAGCAGAGCTGTCAGGCAATTCCAGATc                                                                <  2:581199/150‑1 (MQ=255)                                                                                 gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCg                                                               <  3:140305/150‑1 (MQ=255)                                                                                  ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACTAAGGTTATGCGTTTTATTGAGCTCAACCCCGGTACGCTGTACCAGATCACCTATCTAGTGTATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCACATccc                                                              >  2:597361/1‑148 (MQ=255)                                                                                  ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCa                                                                                                                                   <  2:722597/81‑1 (MQ=255)                                                                                  ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCa                                                                                                                                   >  1:722597/1‑81 (MQ=255)                                                                                   cAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTc                                                             <  2:144970/150‑1 (MQ=255)                                                                                     gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCaa                                                           <  4:636920/150‑1 (MQ=255)                                                                                       aCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa                                                                                              <  1:977659/113‑1 (MQ=255)                                                                                       aCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa                                                                                              >  2:977659/1‑113 (MQ=255)                                                                                         gTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTAc                                                                                                                                     <  1:96689/72‑1 (MQ=255)                                                                                         gTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTAc                                                                                                                                     >  2:96689/1‑72 (MQ=255)                                                                                          tCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATCTAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAggg                                                      >  1:791660/1‑150 (MQ=255)                                                                                              tAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCgca                                                  >  2:195633/1‑150 (MQ=255)                                                                                              tAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCgca                                                  <  1:195633/150‑1 (MQ=255)                                                                                                     aGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggat                                           >  1:390084/1‑150 (MQ=255)                                                                                                         agagATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                       <  2:111189/150‑1 (MQ=255)                                                                                                          gagaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGa                                                                                                                                 <  2:63398/59‑1 (MQ=255)                                                                                                          gagaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGa                                                                                                                                 >  1:63398/1‑59 (MQ=255)                                                                                                          gagaTTATTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggat                                      <  1:605911/150‑1 (MQ=255)                                                                                                           agaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc                                     >  2:1090167/1‑150 (MQ=255)                                                                                                           agaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc                                     <  2:1064218/150‑1 (MQ=255)                                                                                                               tttttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAAc                                 >  1:962063/1‑150 (MQ=255)                                                                                                                     ccAAGGTTAGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGt                           >  4:398634/1‑150 (MQ=255)                                                                                                                             aGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttc                   <  1:616564/150‑1 (MQ=255)                                                                                                                             aGGCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttc                   <  3:489489/150‑1 (MQ=255)                                                                                                                              ggCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTc                                                             >  1:41849/1‑107 (MQ=255)                                                                                                                              ggCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTc                                                             <  2:41849/107‑1 (MQ=255)                                                                                                                              ggCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttct                  >  3:330933/1‑150 (MQ=255)                                                                                                                               gCGTTTTAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTcttctt                 >  3:448704/1‑150 (MQ=255)                                                                                                                                cctggcaaGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACACGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCttt                <  2:714017/144‑1 (MQ=255)                                                                                                                                  ttttAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCa              <  3:398634/150‑1 (MQ=255)                                                                                                                                  ttttAAGGAGCTCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCa              <  2:337913/150‑1 (MQ=255)                                                                                                                                           gCTCAACCCCGGTACGCTGTACGAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTGCAGATCGTCAACAGGGCGTAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCt     <  2:33526/150‑1 (MQ=255)                                                                                                                                             tCAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGa   >  1:1123577/1‑150 (MQ=255)                                                                                                                                              cAACCCCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGGCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGAc  <  4:539209/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGAC  >  NC_000913_3_pae_pgi/3440027‑3440317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑