New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 289269219 (1.100)4 (0.240) 4/260 1.8 17.7% coding (36/1272 nt) ygcN putative oxidoreductase
?NC_000913_3_pae_pgi = 2892701 19 (1.150)coding (45/1272 nt) ygcN putative oxidoreductase
Rejected: Frequency below/above cutoff threshold.

TTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/2892602‑2892692
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtgccTGCAATACCGGCACCAATAATAATAATGTCGCAGTCGTCTTCCA  <  NC_000913_3_pae_pgi/2892701‑2892658
                                                                                                                                       
TTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCCTGCAATACCGGCACCAATAATAATAATGTCGCAGTCGTCTTCCA  >  1:27667/1‑135
TTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCCTGCAATACCGGCACCAATAATAATAATGTCGCAGTCGTCTTCCA  <  2:27667/135‑1
                                                    GACATGGAAGACGACTGCGACATTATTATTATTGGTGCCTGCAATACCGGCAC                                <  3:116121/53‑1
                                                    GACATGGAAGACGACTGCGACATTATTATTATTGGTGCCTGCAATACCGGCAC                                >  4:116121/1‑53
                                                                                                                                       
TTCCCTTAAGTAACGCTATGTTAGGGTGTTGTGTTCTGGATATCTGGGGCATGACATGGAAGACGACTGCGACATTATTATTATTGGTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/2892602‑2892692
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtgccTGCAATACCGGCACCAATAATAATAATGTCGCAGTCGTCTTCCA  <  NC_000913_3_pae_pgi/2892701‑2892658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.