Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_vch_tpiA	257,908	Δ776 bp		[crl]	[crl]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_vch_tpiA	257908–258675	258683	9–776	60 [0]	[0] 59	[crl]	[crl]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_vch_tpiA	= 257907	0 (0.000)	55 (1.520)	46/268	0.0	100%	noncoding (257907/4641682 nt)	repeat_region	repeat region
	?	NC_000913_3_vch_tpiA	258684 =	0 (0.000)					noncoding (258684/4641682 nt)	repeat_region	repeat region

GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTG  >  NC_000913_3_vch_tpiA/258536‑258829                                                                                                                                                     |                                                                                                                                                  cgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                   <  1:455162‑M2/1‑1 (MQ=255)      gtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgaactaggcccgtatattcgtgaaggtaAGTGCa                                                                                                                                              >  2:378643‑M2/144‑149 (MQ=255)        gttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                            <  1:205015‑M2/8‑1 (MQ=255)        gttaaaaatttgctaaatttggccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                            <  1:148689‑M2/8‑1 (MQ=255)         ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                           >  2:240589‑M2/141‑149 (MQ=255)          taaaaatttgctaaattttgccaagttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                          <  2:200821‑M2/10‑1 (MQ=255)              aatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgaaaccattcccgatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                      <  1:332861‑M2/14‑1 (MQ=255)                    ctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                                <  1:243682‑M2/20‑1 (MQ=255)                     taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacactcgaagagcagattgatcaaaaaatttaccgcactatgcccgtatattcgtgaaggtaAGTTCAAGGATACTCGGTtcg                                                                                                                               >  2:345214‑M2/129‑148 (MQ=255)                     taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactacgcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                               >  2:73052‑M2/129‑149 (MQ=255)                     taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtagacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtagattcgtgaaggtaAGTGAAAAGATCATGGATTCt                                                                                                                               >  2:603654‑M2/129‑149 (MQ=255)                         ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                           <  1:240589‑M2/25‑1 (MQ=255)                         ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                           <  2:631853‑M2/25‑1 (MQ=255)                         ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                           >  1:38069‑M2/125‑149 (MQ=255)                          tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                          <  1:25764‑M2/26‑1 (MQ=255)                           ttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                         <  1:385556‑M2/27‑1 (MQ=255)                              ccaatttggtaaaacagttgcatcacaacagcagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                      >  1:514569‑M2/120‑149 (MQ=255)                                  tttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt                                                                                                                  >  1:596880‑M2/116‑149 (MQ=255)                                   ttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTg                                                                                                                 >  1:388613‑M2/115‑149 (MQ=255)                                      gtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAATCTTTttagattgcttggat                                                                                                              >  2:124443‑M2/112‑142 (MQ=255)                                        aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                            <  2:388613‑M2/40‑1 (MQ=255)                                               ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGTCTGTATGCGTc                                                                                                     >  1:289775‑M2/103‑149 (MQ=255)                                                  catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                                                                  <  2:596880‑M2/50‑1 (MQ=255)                                                    tcaaaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                <  2:63780‑M2/52‑1 (MQ=255)                                                       caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                                                                             >  1:2201‑M2/95‑149 (MQ=255)                                                             gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                                                                       >  1:189101‑M2/89‑149 (MQ=255)                                                                      atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                              <  2:69239‑M2/70‑1 (MQ=255)                                                                            ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                                                                            >  2:624604‑M2/74‑97 (MQ=255)                                                                            ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                                                                            <  1:624604‑M2/24‑1 (MQ=255)                                                                                cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                    >  1:117201‑M2/70‑149 (MQ=255)                                                                                   gtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                                 <  1:585727‑M2/83‑1 (MQ=255)                                                                                   cgggcccccctaagagcagagtgatcaaaaaatttacctcacgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                                 <  1:202182‑M2/83‑1 (MQ=255)                                                                                    tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                                <  1:546876‑M2/84‑1 (MQ=255)                                                                                        cacccgaagggcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg                                                            <  2:299129‑M2/88‑1 (MQ=255)                                                                                               agagcagattgatcaatagatttaccgcactaggcctggatattcgtgaaggtaAGTGGAATGAGAATCGATTCTTGTTTGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCTCCGGAAGTGCGTGAATTCTGGGGCTGGtggatg                                                     <  2:632690‑M2/95‑1 (MQ=255)                                                                                                  gcagattgatcaaaaaatttcccgcactaggcccgtataaaagcgaaagtaTGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg                                                  <  1:53295‑M2/98‑1 (MQ=255)                                                                                                     gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                >  2:570430‑M2/49‑84 (MQ=255)                                                                                                     gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                <  1:570430‑M2/36‑1 (MQ=255)                                                                                                        tgatcaaaaaattgaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                            <  2:563692‑M2/104‑1 (MQ=255)                                                                                                             aaaaaatttaccggacgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                       <  2:113123‑M2/109‑1 (MQ=255)                                                                                                             aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                       >  2:350896‑M2/41‑149 (MQ=255)                                                                                                             aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGCGGCTGGTGTATGGAGCTGTGAGCGCa                                       >  2:382419‑M2/41‑149 (MQ=255)                                                                                                             aaaaaatttaccgcactaggcccgtagattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAACGGAAGTGCGTGAATTCTGGGGCTGGTGGATAGAGCTGGAagcgcc                                       >  2:8939‑M2/41‑148 (MQ=255)                                                                                                             aaaaaagttaccgcactaggcccgtatattcgtgaaggtaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGACGTTCGTTAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                       <  2:286421‑M2/109‑1 (MQ=255)                                                                                                              aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                      >  1:545789‑M2/40‑149 (MQ=255)                                                                                                                        cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                            <  1:489068‑M2/120‑1 (MQ=255)                                                                                                                        cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACAGGCACCGGAAATGCGTGATTTCAGGGGGTGGTGGATGCAGCTGGAACCGCGGGGACCCCGtt                            >  2:634366‑M2/30‑149 (MQ=255)                                                                                                                               ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                     >  1:63887‑M2/23‑149 (MQ=255)                                                                                                                                gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                    >  1:98758‑M2/22‑149 (MQ=255)                                                                                                                                 cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                   >  1:574905‑M2/21‑149 (MQ=255)                                                                                                                                 cccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGCTGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa                   >  1:574924‑M2/21‑149 (MQ=255)                                                                                                                                    gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGtt                >  1:562987‑M2/18‑149 (MQ=255)                                                                                                                                     tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa               >  2:12159‑M2/17‑149 (MQ=255)                                                                                                                                      atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc              <  2:562987‑M2/134‑1 (MQ=255)                                                                                                                                            gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt        <  1:12159‑M2/140‑1 (MQ=255)                                                                                                                                             tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg       <  1:566853‑M2/141‑1 (MQ=255)                                                                                                                                             tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGAGGCTGGTGGATGGAGCTGGAAGAGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg       >  2:213336‑M2/9‑149 (MQ=255)                                                                                                                                             ggaaggtaAGCGCAAAGATAATCGATTCCTTTTCGATTGACTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg       <  2:554958‑M2/141‑1 (MQ=255)                                                                                                                                                  gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGCTTTACCTACAGTTACCAGTTTTGTCTg  >  1:14077‑M2/4‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTG  >  NC_000913_3_vch_tpiA/258536‑258829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑