Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,726,999 | 0 | C | G | 54.5% | ‑6.1 / 13.8 | 11 | noncoding (2186/2904 nt) | rrlG | 23S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base C (1/4); new base G (6/0); total (7/4) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAA > NC_000913/2726918‑2727087 | ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:1061390/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:1758657/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:1758665/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:199549/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:479972/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACAGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGcc > 1:515914/4‑141 (MQ=0) cactCACACTACCCACCAGACACTGTCCGCACCCCGGATTACCGGTCAACGAAAGAACAACACACAATAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCTAACTTCAAAGCGTCCCACCTATCCTacac < 2:74030/138‑1 (MQ=1) gtaaaaCTACCCACCAGACACTGTAATAAACACAGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACAt > 2:272214/4‑141 (MQ=1) acgaCCCCCCAGACACTGTCCGCAACCCGGATTACGGGTCACCGATAGAACAACACCCATTCACGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACGTCTCCTACACATCAAgg < 2:1661151/138‑1 (MQ=1) cccccaGACCCCGTCCGCAACCCGGATGACGGGACAACCTGCGAACCTCACCCAAGACCGGGTGGAAGTACAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCGTCCCCCCTTTCCTACACATCAAGGCTCaa < 2:593674/138‑1 (MQ=0) cccccaGACACTGTCCGCAACCCGCATTACGGGTCAACGTTAGAACATCAACCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAACGCCTCCCACCTATCCTACACATCAAGGCTCaa < 2:596885/138‑1 (MQ=0) | TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAA > NC_000913/2726918‑2727087 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |