Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,726,9990CG54.5% ‑6.1 / 13.8 11noncoding (2186/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAA  >  NC_000913/2726918‑2727087
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ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                               >  1:1061390/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                               >  1:1758657/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                               >  1:1758665/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                               >  1:199549/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                               >  1:479972/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACAGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGcc                               >  1:515914/4‑141 (MQ=0)
                 cactCACACTACCCACCAGACACTGTCCGCACCCCGGATTACCGGTCAACGAAAGAACAACACACAATAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCTAACTTCAAAGCGTCCCACCTATCCTacac              <  2:74030/138‑1 (MQ=1)
                   gtaaaaCTACCCACCAGACACTGTAATAAACACAGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACAt            >  2:272214/4‑141 (MQ=1)
                        acgaCCCCCCAGACACTGTCCGCAACCCGGATTACGGGTCACCGATAGAACAACACCCATTCACGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACGTCTCCTACACATCAAgg       <  2:1661151/138‑1 (MQ=1)
                             cccccaGACCCCGTCCGCAACCCGGATGACGGGACAACCTGCGAACCTCACCCAAGACCGGGTGGAAGTACAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCGTCCCCCCTTTCCTACACATCAAGGCTCaa  <  2:593674/138‑1 (MQ=0)
                             cccccaGACACTGTCCGCAACCCGCATTACGGGTCAACGTTAGAACATCAACCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAACGCCTCCCACCTATCCTACACATCAAGGCTCaa  <  2:596885/138‑1 (MQ=0)
                                                                                 |                                                                                        
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAA  >  NC_000913/2726918‑2727087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: