Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0460AC45.5% ‑6.2 / 10.3 12noncoding (2139/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base C (5/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCC  >  NC_000913/2726918‑2727137
                                                                                                                                |                                                                                           
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                 >  1:1061390/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                 >  1:1758657/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                 >  1:1758665/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                 >  1:199549/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                 >  1:479972/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACAGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGcc                                                                                 >  1:515914/4‑141 (MQ=0)
                 cactCACACTACCCACCAGACACTGTCCGCACCCCGGATTACCGGTCAACGAAAGAACAACACACAATAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCTAACTTCAAAGCGTCCCACCTATCCTacac                                                                <  2:74030/138‑1 (MQ=1)
                   gtaaaaCTACCCACCAGACACTGTAATAAACACAGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACAt                                                              >  2:272214/4‑141 (MQ=1)
                        acgaCCCCCCAGACACTGTCCGCAACCCGGATTACGGGTCACCGATAGAACAACACCCATTCACGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACGTCTCCTACACATCAAgg                                                         <  2:1661151/138‑1 (MQ=1)
                             cccccaGACCCCGTCCGCAACCCGGATGACGGGACAACCTGCGAACCTCACCCAAGACCGGGTGGAAGTACAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCGTCCCCCCTTTCCTACACATCAAGGCTCaa                                                    <  2:593674/138‑1 (MQ=0)
                             cccccaGACACTGTCCGCAACCCGCATTACGGGTCAACGTTAGAACATCAACCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAACGCCTCCCACCTATCCTACACATCAAGGCTCaa                                                    <  2:596885/138‑1 (MQ=0)
                                                                               accaatAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGcc  <  1:140884/136‑1 (MQ=1)
                                                                                                                                |                                                                                           
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCC  >  NC_000913/2726918‑2727137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: