Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 700,588 (T)6→5 coding (1007/1221 nt) nagC ← DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913700,5830T.77.4% 255.6 / 119.8 107coding (1012/1221 nt)nagCDNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base T (16/8);  new base . (35/47);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 6.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 109 total aligned reads displayed.

TTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGT  >  NC_000913/700445‑700714
                                                                                                                                          |                                                                                                                                   
ttGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAAttt                                                                                                                                   >  2:1808235/1‑141 (MQ=255)
ttGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAAttt                                                                                                                                   >  2:1808236/1‑141 (MQ=255)
             aTCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAAt                                                                                                                     <  1:710597/141‑1 (MQ=255)
             aTCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAAt                                                                                                                     <  1:710495/141‑1 (MQ=255)
             aTCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAAt                                                                                                                     <  1:290227/141‑1 (MQ=255)
                cAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATaa                                                                                                                   >  1:1814067/1‑141 (MQ=255)
                cAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAg                                                                                                                  >  1:451407/1‑141 (MQ=255)
                cAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAg                                                                                                                  >  1:1545649/1‑141 (MQ=255)
                cAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCAGGTTATTAATGCAGCTTTCAATAGCAGTGAGCAGCACTTTATCGGCTACGGTGATTTCACCGGCAATAACAATTTTAGAGGATAAAATAAg                                                                                                                  >  1:452276/1‑141 (MQ=255)
                   cTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTg                                                                                                               <  2:1128355/141‑1 (MQ=255)
                    tCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGa                                                                                                              >  2:1773684/1‑141 (MQ=255)
                      aGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGAt                                                                                                             >  2:767153/1‑141 (MQ=255)
                            tACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATg                                                                                                      >  1:200368/1‑141 (MQ=255)
                            tACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATg                                                                                                      >  2:1404782/1‑141 (MQ=255)
                            tACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATg                                                                                                      >  1:354998/1‑141 (MQ=255)
                            tACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATg                                                                                                      >  2:1766371/1‑141 (MQ=255)
                                 ccGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCGGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGAt                                                                                                 >  1:553901/1‑141 (MQ=255)
                                 ccGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGTCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGAt                                                                                                 <  2:1558354/141‑1 (MQ=255)
                                 ccGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAATTAAGTTGATAGCAATGGCGAt                                                                                                 >  2:1676575/1‑141 (MQ=255)
                                 ccGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGAt                                                                                                 <  2:1289540/141‑1 (MQ=255)
                                  cGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGAt                                                                                                 <  1:1708635/141‑1 (MQ=255)
                                            gTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCa                                                                                      <  2:1205428/141‑1 (MQ=255)
                                                 aaaCGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCACTAACAATGTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATg                                                                                  >  1:1071010/1‑141 (MQ=255)
                                                 aaaCGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGagg                                                                                  >  1:1071009/1‑139 (MQ=255)
                                                 aaaCGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATg                                                                                  >  1:1071314/1‑141 (MQ=255)
                                                 aaaCGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATga                                                                                 >  2:1384213/1‑141 (MQ=255)
                                                   aCGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATgacg                                                                               <  1:1275915/141‑1 (MQ=255)
                                                     gCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATgacgac                                                                             >  2:1643746/1‑141 (MQ=255)
                                                     gCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATgacgac                                                                             <  2:1473513/141‑1 (MQ=255)
                                                     gCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATgacgac                                                                             <  1:1124183/141‑1 (MQ=255)
                                                     gCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATgacgac                                                                             <  1:1508846/141‑1 (MQ=255)
                                                     gCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATgacgac                                                                             <  1:1771829/141‑1 (MQ=255)
                                                           aGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACAt                                                                       <  2:1569879/141‑1 (MQ=255)
                                                           aGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACAt                                                                       <  1:89078/141‑1 (MQ=255)
                                                             cgcCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATAc                                                                     <  1:1168921/141‑1 (MQ=255)
                                                                cTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCa                                                                  >  2:645920/1‑141 (MQ=255)
                                                                cTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCa                                                                  >  1:486234/1‑141 (MQ=255)
                                                                cTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCa                                                                  >  1:1573530/1‑141 (MQ=255)
                                                                cTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCa                                                                  >  1:1573483/1‑141 (MQ=255)
                                                                cTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAAGTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCa                                                                  >  1:486263/1‑141 (MQ=255)
                                                                     tATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTa                                                              <  1:938309/141‑1 (MQ=255)
                                                                      aTTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTAc                                                             <  1:1165847/141‑1 (MQ=255)
                                                                         aaTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTcc                                                         <  2:1150029/141‑1 (MQ=255)
                                                                         aaTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTcc                                                         <  2:196317/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTcc                                                         <  2:242180/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        <  1:944611/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        <  2:831716/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        <  2:1313083/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        <  2:1312923/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        <  2:831688/141‑1 (MQ=255)
                                                                          aTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        <  2:658457/141‑1 (MQ=255)
                                                                           tGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGACTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCg                                                        >  1:559610/1‑141 (MQ=255)
                                                                            gCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACACGTTTTTGCGGATTAAATAAGTTGTGAGCAATGGCGATGGTTTTACCCAGATGTCGTCCGACATTCTCAGTTACTTCCGa                                                       >  1:1127025/1‑141 (MQ=255)
                                                                            gCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGAc                                                      >  2:229393/1‑141 (MQ=255)
                                                                            gCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGAc                                                      >  2:229397/1‑141 (MQ=255)
                                                                            gCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGAc                                                      >  2:1895473/1‑141 (MQ=255)
                                                                             cAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGAc                                                      >  1:961295/1‑141 (MQ=255)
                                                                             cAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGAc                                                      >  1:961265/1‑141 (MQ=255)
                                                                              aGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAGCAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGc                                                    >  1:814858/1‑141 (MQ=255)
                                                                              aGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGc                                                    >  1:38513/1‑141 (MQ=255)
                                                                              aGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAAGAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGc                                                    >  1:814977/1‑141 (MQ=255)
                                                                                 tttCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCa                                                  <  1:948063/141‑1 (MQ=255)
                                                                                 tttCAATAGCAGGGAGCAGCACTTTATCGGATTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCa                                                  <  1:779839/141‑1 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGAGCGACATAGTCAATTAGTTCCGACGCCAGACTTTCg                                          >  1:1113650/1‑141 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTCTCg                                          >  2:1002430/1‑141 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCg                                          >  1:1113658/1‑141 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCg                                          >  1:1538252/1‑141 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCg                                          >  1:1538267/1‑141 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCg                                          >  1:1538511/1‑141 (MQ=255)
                                                                                         gCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAAGTACTTCCGACGCCAGACTATCg                                          >  2:1002425/1‑141 (MQ=255)
                                                                                              gAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTg                                    >  1:621563/1‑141 (MQ=255)
                                                                                                      cTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGc                            >  1:1027813/1‑141 (MQ=255)
                                                                                                             ggCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg                     >  2:874390/1‑141 (MQ=255)
                                                                                                             ggCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg                     >  2:874412/1‑141 (MQ=255)
                                                                                                             ggCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg                     >  1:381671/1‑141 (MQ=255)
                                                                                                             ggCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAg                     >  2:1933246/1‑141 (MQ=255)
                                                                                                              gCTTCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGa                    <  2:1654912/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:553901/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:1021311/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:604645/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  1:310854/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:1315790/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:1315392/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:124998/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:1158928/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:1091404/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  1:833001/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCAAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:939821/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATCTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:125968/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATCTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:125961/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATCTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:125949/141‑1 (MQ=255)
                                                                                                                ttCGGTGATTTCACCGGCAATAACAATCTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:125897/141‑1 (MQ=255)
                                                                                                                 tCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATa                  <  2:1038617/141‑1 (MQ=255)
                                                                                                                        tttCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt          <  2:1513916/141‑1 (MQ=255)
                                                                                                                        tttCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt          >  1:479244/1‑141 (MQ=255)
                                                                                                                        tttCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt          <  2:863612/141‑1 (MQ=255)
                                                                                                                        tttCACCGGCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGAt          <  2:1512244/141‑1 (MQ=255)
                                                                                                                               ggCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCa    <  1:1026660/141‑1 (MQ=255)
                                                                                                                                gCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt  <  2:1680408/141‑1 (MQ=255)
                                                                                                                                gCAATAACAATTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAgt  >  2:977111/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                   
TTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGT  >  NC_000913/700445‑700714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: