Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,726,996 | 0 | A | . | 70.0% | ‑6.8 / 10.3 | 10 | noncoding (2189/2904 nt) | rrlG | 23S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (1/6); total (3/7) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.83e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAA > NC_000913/2726918‑2727111 | ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:1024598/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:1787951/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCGACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:1748559/4‑141 (MQ=0) acgaCCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATAAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAgg < 1:728802/138‑1 (MQ=1) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:686659/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:686761/138‑1 (MQ=0) cgataaggggTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTaa < 1:159159/134‑1 (MQ=0) cgataaggggTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTaa < 1:508897/134‑1 (MQ=0) cgataaggggTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTaa < 1:508955/134‑1 (MQ=0) gataaggggTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTCAGTGCCAAGCTGTAGTaaa < 1:1301090/135‑1 (MQ=0) | TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAA > NC_000913/2726918‑2727111 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |