Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,412,7810CG35.4% 86.7 / 12.4 52S132C (TCT→TGT) yjfMDUF1190 domain‑containing protein YjfM
Reads supporting (aligned to +/- strand):  ref base C (4/27);  new base G (17/0);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.41e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCT  >  NC_000913/4412642‑4412914
                                                                                                                                           |                                                                                                                                       
aTTGGCGCGCGGGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGTACTGTCAGTCTAAGTACGCAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCt                                                                                                                                        <  2:1271679/141‑1 (MQ=255)
     cgcgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑tt                                                                                                                                  <  1:1045212/141‑1 (MQ=255)
         cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑tttttt                                                                                                                              >  1:152400/1‑140 (MQ=255)
                      acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGGGTGA‑TTTTTTTTTATCCGGGttt                                                                                                                 >  1:595474/1‑140 (MQ=255)
                      acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGT‑TGTTTTTTTATCCggggtt                                                                                                                 >  1:786310/1‑138 (MQ=255)
                      acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGG‑TTTTTTTTTATCCGGGTTa                                                                                                                 >  1:255967/1‑141 (MQ=255)
                      acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGA‑TTTTTGTTAAGCCggggtt                                                                                                                 >  1:786202/1‑138 (MQ=255)
                       cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTAt                                                                                                                <  2:765131/141‑1 (MQ=255)
                                        aTGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGT‑TTTTTGTTTATCCGGGTTATTAGTAAAGATCGCGATg                                                                                               >  2:1689771/1‑141 (MQ=255)
                                               tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt                                                                                       <  2:1233718/141‑1 (MQ=255)
                                               tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt                                                                                       <  2:983163/141‑1 (MQ=255)
                                               tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt                                                                                       <  2:177252/141‑1 (MQ=255)
                                               tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt                                                                                       <  2:1233725/141‑1 (MQ=255)
                                               tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtt                                                                                       <  1:295288/141‑1 (MQ=255)
                                               tGTTCCGAAGAATAGGACTCAGCAGAACTGTCAGTCTAAGTACGTAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGAGGAGCC‑gtt                                                                                       <  2:1135617/141‑1 (MQ=255)
                                               tGTTCCGAACAATATGACTCAGCAGAACTGTCAGTCTAAGTACGTAAATAGCTACTACGCCAATGTTGAACAGAGATGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGCGTTATTCGTAAAGATCGCGATGAGCC‑gtt                                                                                       <  2:1135611/141‑1 (MQ=255)
                                                       agaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGA‑ATTTTTGTAATCCGGGTTAATCGTAAATATCGCGATGAGCC‑GTTTGGTtata                                                                               >  2:386867/1‑141 (MQ=255)
                                                          atatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGATTTTTTTTTATGCCGGG‑TGTTTGTAAATATCGCGATTAGCCGGTGTGTTTaaaaa                                                                             >  1:893427/1‑140 (MQ=255)
                                                          atatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGT‑TTTTTTTTAATCCGGGGTTTTCGTTAAGATAGCGATGAGCC‑GTTTGTTTTTaaaa                                                                            >  1:816866/1‑139 (MQ=255)
                                                          atatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTTTTTAGCCCGGGTATTAGTAAAAATCGCGGTTAGCC‑GGTTTTTTTTaaaa                                                                            >  1:893426/1‑139 (MQ=255)
                                                             tGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAACGATCGCGATGAGCC‑GTTTGTTTATAACAgcg                                                                         <  2:352807/141‑1 (MQ=255)
                                                             tGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACGACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAgcg                                                                         <  2:352610/141‑1 (MQ=255)
                                                              gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGA‑TTTTTGTTAATCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAgcgg                                                                        >  2:1579350/1‑141 (MQ=255)
                                                                  cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGt                                                                    <  1:650381/141‑1 (MQ=255)
                                                                  cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGG‑TTTTTTTTTAGGCGGGGTTTTAGGAAAGATCGCGGTGATCC‑GGTTTTTTTTTAAAGcggcggg                                                                    >  1:1368854/1‑140 (MQ=255)
                                                                  cagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGA‑TTTTTTTTAATCCGGGTTATTAGTAAAGATCGCGAAGATCC‑GGTTGTTTTTAAAAGcggcggg                                                                    >  1:1922392/1‑140 (MQ=255)
                                                                            gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGGGGT‑TTTTTTTTAATCCGGGGTTTTCGTAAAGATCGCGATGATCCGGTTTGTTT‑TAAAAGCGGCGGGTTCTAttttt                                                          >  1:1393071/1‑140 (MQ=255)
                                                                              cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATAGCGATGAGCC‑GTTTGTTTATAAAAGCGGCGGTTCCTCTTTTTCt                                                        >  2:1592361/1‑141 (MQ=255)
                                                                              cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGT‑TTTTTGTTAATCCGGGTTATTCGTTAATATAGCGATGAGCC‑GGTTGTTTATAACAGCGGCGGTTACTCtttttgt                                                        >  2:1592430/1‑138 (MQ=255)
                                                                                gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTc                                                      <  1:1796721/141‑1 (MQ=255)
                                                                                gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTc                                                      <  1:571012/141‑1 (MQ=255)
                                                                                 tCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTTATCCGGGTTATTAGTAAAGAACGCGATGTGCC‑GGTTGTTTTTTACAGCGGCGGTTACTCTGTTTCttgg                                                     >  2:577047/1‑139 (MQ=255)
                                                                                    aaGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCgcgc                                                  <  2:430375/141‑1 (MQ=255)
                                                                                      gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGccc                                                <  2:1193415/141‑1 (MQ=255)
                                                                                      gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTAATCCGGGGTATTGGTAAAGATAGCGATTAGCC‑GTTTGTTTATAACATCGGCGGTTGCTCTTTTTGTTAGCGccc                                                >  2:713841/1‑141 (MQ=255)
                                                                                          gAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTATGGA‑TTTTTTTTTAGCCCGGGTAATAGTAAAAATCGCCAAGAAAC‑GTTTTTTTATAAAAGCGGCGGGTACTATTTTTCTTAGCTCCCAgac                                            >  2:326923/1‑139 (MQ=255)
                                                                                           aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCt                                           <  2:1712780/141‑1 (MQ=255)
                                                                                                 gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgca                                     <  1:1633283/141‑1 (MQ=255)
                                                                                                         gACAATGTTGAACAGAGTTGGATCCCGGACGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                             <  1:484369/141‑1 (MQ=255)
                                                                                                           cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg                           <  2:1355922/141‑1 (MQ=255)
                                                                                                           cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTgg                           <  2:1698407/141‑1 (MQ=255)
                                                                                                           cAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGA‑TTTTTGTTAAACCGGGGTATTCGGAAATAACGCGAATAGCC‑GGTTGGTTATAAAAACGGCCGTTGCGCAGTTGGATAGCGCCCCGGCAGGCGCAGCACATCTgg                           >  2:1325756/1‑141 (MQ=255)
                                                                                                                 tGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCCGGGTATTCGTAAAGATCGCGATTAGCC‑CTTTGTTTATAACAGCGGCGGTTACTCTTTTTCTTCGCGCCCAGTCTGGCGCCGCACATCTGGTGATTa                     >  2:1181980/1‑141 (MQ=255)
                                                                                                                 tGAACAGAGTTGGATCCCGGTCGTTTATGGA‑TTATTGTTAAGCCGGGTTATTCATAAATATCCCGTTGATCC‑GTTTTTTTATAACACCGGCGGTTCCAAATTTTCGTAGCCCCCAGTCTGGCGCCTCACTTCTGGTGATTa                     >  2:1181979/1‑141 (MQ=255)
                                                                                                                       gagTTGGATCCCGGTCGTTTGTGGA‑TTTTTTTTAATCCGGGGTTTTGGTTAATATCGCGGTGAGCC‑GGTTTTTTATAAAAGCGGCGGTTCCTATTTTTGTTAGCGCCCAGTCTGGCGCAGCCCGTCTGGTGATTACTCATg               >  2:10157/1‑141 (MQ=255)
                                                                                                                         gTTGGATCCCGGTCGGTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGgc             <  2:653771/141‑1 (MQ=255)
                                                                                                                              aTCCCGGTCGTGTGTGGA‑TTTTTGTTTATCCGGGTTTTTCGTAAAGATCGCCATGAGCC‑GGTTTTTTATAAAAGCGGCGGTTCCTCTTTTGCTTAGCGCCCAGTCTGGCGCCGCACTTATGGTGATTAATCCAGGCGCGcc        >  2:123922/1‑141 (MQ=255)
                                                                                                                                 ccGGTCGTTGAGGGA‑TTTTTTTTAAGCCGGGTTATTCGTGAAGTTCGCGTTGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCACGTTTGGTTCGCCCCCAGTAGGGGGCAGCACGGCGGGGGAGTACAACGGGCGCTCCCGt     >  2:1389990/1‑141 (MQ=255)
                                                                                                                                    gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt  <  2:1146952/141‑1 (MQ=255)
                                                                                                                                    gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt  <  2:519422/141‑1 (MQ=255)
                                                                                                                                    gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt  <  2:1614310/141‑1 (MQ=255)
                                                                                                                                    gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCt  <  2:1146889/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
ATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCT  >  NC_000913/4412642‑4412914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑