Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,968,971 Δ1 bp coding (1056/1104 nt) rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,968,9680A.82.6% 659.7 / 190.2 219coding (1053/1104 nt)rfeUDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (19/19);  new base . (64/116);  total (84/135)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 222 total aligned reads displayed.

CTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACC  >  NC_000913/3968828‑3969105
                                                                                                                                            |                                                                                                                                         
cTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTa                                                                                                                                           <  1:915372/141‑1 (MQ=255)
       ccATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCa                                                                                                                                    >  2:1836731/1‑141 (MQ=255)
       ccATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAg                                                                                                                                   >  2:1455503/1‑141 (MQ=255)
       ccATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAg                                                                                                                                   <  2:575757/141‑1 (MQ=255)
        cATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGa                                                                                                                                  >  1:28077/1‑141 (MQ=255)
         aTTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGa                                                                                                                                  >  1:1219686/1‑141 (MQ=255)
         aTTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGAc                                                                                                                                 >  1:1745413/1‑141 (MQ=255)
         aTTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGAc                                                                                                                                 <  1:2092895/141‑1 (MQ=255)
            ggCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGc                                                                                                                              <  2:21253/141‑1 (MQ=255)
              cGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGt                                                                                                                            >  2:376254/1‑141 (MQ=255)
              cGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGt                                                                                                                            >  1:2106536/1‑141 (MQ=255)
              cGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGt                                                                                                                            >  2:2371779/1‑141 (MQ=255)
               gTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTa                                                                                                                           >  1:816036/1‑141 (MQ=255)
               gTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTa                                                                                                                           >  1:2127883/1‑141 (MQ=255)
                tGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTa                                                                                                                           <  1:678621/141‑1 (MQ=255)
                   tGGCAGAATATTCTCCTTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:535205/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1783248/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:380968/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1410514/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1048687/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2453796/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1069614/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1152885/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:119284/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2561617/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1953233/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1741678/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2028553/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2435567/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2339915/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  1:1370920/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2324029/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:246752/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2208901/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:947552/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:1989403/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2037379/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:247302/141‑1 (MQ=255)
                   tGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:209249/141‑1 (MQ=255)
                    ggCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGaaa                                                                                                                       <  1:984687/141‑1 (MQ=255)
                    ggCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGaaa                                                                                                                       <  1:1887422/141‑1 (MQ=255)
                    ggCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:2336573/141‑1 (MQ=255)
                     gCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATc                                                                                                                     <  2:2571599/141‑1 (MQ=255)
                     gCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATc                                                                                                                     <  2:1253765/141‑1 (MQ=255)
                     gCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATc                                                                                                                     <  2:2309703/141‑1 (MQ=255)
                      cAGAATATTCTCAATATGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCGATGGATATTGCATTAAGCGTGCCTGGAAAGGTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCg                                                                                                                    <  2:1385728/141‑1 (MQ=255)
                              tctcATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCa                                                                                                            >  2:408937/1‑141 (MQ=255)
                              tctcATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCa                                                                                                            >  2:141651/1‑141 (MQ=255)
                              tctcATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCa                                                                                                            >  2:281509/1‑141 (MQ=255)
                              tctcATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCGTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGc                                                                                                             >  2:1109046/1‑141 (MQ=255)
                                 cATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGcc                                                                                                         <  2:1196986/141‑1 (MQ=255)
                                 cATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGcc                                                                                                         <  2:1723092/141‑1 (MQ=255)
                                  aTTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACTCAGACTGCGTAGAAATCGTGGTGGCAGccc                                                                                                        >  2:1812150/1‑141 (MQ=255)
                                  aTTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGccc                                                                                                        <  1:258426/141‑1 (MQ=255)
                                           cGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAAc                                                                                               <  1:1408075/141‑1 (MQ=255)
                                           cGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAAc                                                                                               <  2:2319992/141‑1 (MQ=255)
                                                     cATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCaaataaat                                                                                      <  1:1836072/141‑1 (MQ=255)
                                                      gaccTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       >  1:711178/4‑106 (MQ=255)
                                                      gaccTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGaaa                                                                                                                       <  2:711178/103‑1 (MQ=255)
                                                        gCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAg                                                                                   >  1:2390817/1‑141 (MQ=255)
                                                        gCTGGTGCTATTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAgg                                                                                  <  2:271367/141‑1 (MQ=255)
                                                            gTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGAtgt                                                                              >  1:1133951/1‑141 (MQ=255)
                                                                     ttGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGacac                                                                      >  2:2163160/1‑141 (MQ=255)
                                                                      tGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGAcaca                                                                     <  1:1102294/141‑1 (MQ=255)
                                                                      tGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACaa                                                                    <  1:1424727/141‑1 (MQ=255)
                                                                      tGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACaa                                                                    <  1:1107421/141‑1 (MQ=255)
                                                                         tAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCa                                                                 <  2:1798595/141‑1 (MQ=255)
                                                                           gCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGGGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAAt                                                               >  2:414161/1‑141 (MQ=255)
                                                                            cATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATg                                                              <  2:1306804/141‑1 (MQ=255)
                                                                                        aTGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAAcc                                                   >  1:379807/1‑141 (MQ=255)
                                                                                             tatTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGa                                             >  2:1584846/1‑141 (MQ=255)
                                                                                               ttGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAg                                           >  2:1819044/1‑141 (MQ=255)
                                                                                                tGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGa                                          >  2:1479526/1‑141 (MQ=255)
                                                                                                 gCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGAc                                         <  1:1606730/141‑1 (MQ=255)
                                                                                                               tGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAAc                            <  1:1036801/141‑1 (MQ=255)
                                                                                                                  aaaGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGa                        <  1:324697/141‑1 (MQ=255)
                                                                                                                   aaGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGat                       <  1:1344752/141‑1 (MQ=255)
                                                                                                                   aaGTTGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGat                       <  1:1515197/141‑1 (MQ=255)
                                                                                                                       tGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTc                   >  2:477041/1‑141 (MQ=255)
                                                                                                                       tGCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTc                   >  2:984497/1‑141 (MQ=255)
                                                                                                                        gCTCGCTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCg                  <  2:18564/141‑1 (MQ=255)
                                                                                                                             cTTTATTAAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGgt             <  1:871028/141‑1 (MQ=255)
                                                                                                                              tttATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGgt             <  1:1836725/141‑1 (MQ=255)
                                                                                                                                 attaAGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGgttgtt         <  1:1851316/141‑1 (MQ=255)
                                                                                                                                  ttaAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGgttgtt         <  1:2294911/141‑1 (MQ=255)
                                                                                                                                    aaGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAACTGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCg      <  2:1668502/141‑1 (MQ=255)
                                                                                                                                    aaGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCg      <  1:1851228/141‑1 (MQ=255)
                                                                                                                                     aGCGCGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGt     >  1:2545573/1‑141 (MQ=255)
                                                                                                                                      gcgcGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTa    <  1:1615835/141‑1 (MQ=255)
                                                                                                                                      gcgcGTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTa    <  2:1609175/141‑1 (MQ=255)
                                                                                                                                       cgcgTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTa    <  1:104651/141‑1 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:127887/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:1874600/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:617362/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:2512183/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:912748/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:913009/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:206424/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTAc   >  2:2261886/1‑141 (MQ=255)
                                                                                                                                       cgcgTAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGGGGGTTGTTTCGTAc   >  2:1222287/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
CTCGCTTCCATTGGCGTGCTGGCAGAATATTCTCATTTTGTCCCGGAGTGGGTCATGCTGGTGCTCTTTTTGCTAGCATTCTTCCTCTATGGATATTGCATTAAGCGTGCCTGGAAAGTTGCTCGCTTTATTAAGCGCGTAAAACGCAGACTGCGTAGAAATCGTGGTGGCAGCCCCAATTTAACCAAATAAATGAGGATGTGATGACACAACCAATGCCTGGGAAACCGGCCGAAGACGCTGAAAATGAACTGGATATTCGTGGGTTGTTTCGTACC  >  NC_000913/3968828‑3969105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: