Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,3200CA34.0% 201.5 / 37.4 151V181F (GTT→TTT) lacAgalactoside O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (56/43);  new base A (51/0);  total (108/43)
Fisher's exact test for biased strand distribution p-value = 3.67e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 152 total aligned reads displayed.

GATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGC  >  NC_000913/361180‑361457
                                                                                                                                            |                                                                                                                                         
gATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAAc                                                                                                                                           >  1:1650533/1‑141 (MQ=255)
     ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTGGCGAATTACCCGAc                                                                                                                                      >  1:872460/1‑141 (MQ=255)
     ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAACCCGAc                                                                                                                                      >  2:2138275/1‑141 (MQ=255)
              aGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGc                                                                                                                             >  1:2237484/1‑141 (MQ=255)
               gATCGCTGACCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGcc                                                                                                                            >  1:1244148/1‑141 (MQ=255)
               gATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGCATAAACCGACAAGGAAAGcc                                                                                                                            >  1:1244187/1‑141 (MQ=255)
               gATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAAAAACCCCACAAGGAAAGcc                                                                                                                            >  1:912224/1‑141 (MQ=39)
               gATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGAAAAAACCGACAAGGAACGcc                                                                                                                            >  1:435186/1‑141 (MQ=39)
                aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAAccccc                                                                                                                           >  2:1245929/1‑140 (MQ=255)
                aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAAACCGACAAGGAACCCCa                                                                                                                           >  1:893375/1‑141 (MQ=255)
                      gAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAAAGCCAcccccc                                                                                                                     >  1:1716252/1‑141 (MQ=39)
                          ttGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCacga                                                                                                                 <  1:1187250/141‑1 (MQ=255)
                            gTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAAAGCCAGCCGCCacgacg                                                                                                               >  2:2210637/1‑141 (MQ=255)
                                gCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCCACAAGGAACGCCAGCCCCCACGACGTTtg                                                                                                           >  1:1986508/1‑141 (MQ=255)
                                gCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCCCCACGACGTTtg                                                                                                           >  1:1590268/1‑141 (MQ=255)
                                gCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtg                                                                                                           >  2:782513/1‑141 (MQ=255)
                                         gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGCATGt                                                                                                  >  2:693268/1‑141 (MQ=255)
                                         gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCGACAAGGAACGCCAGCCGCCCCCACGGTTTGTGGTAttt                                                                                                  >  1:1015133/1‑139 (MQ=255)
                                         gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGTAttt                                                                                                  >  2:693522/1‑139 (MQ=255)
                                         gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTAATTGGCGAAAAAACCCACAACGAAACCCACCCGCCCCCACGTTTTGTTGAAttt                                                                                                  >  1:2238947/1‑139 (MQ=255)
                                         gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTCGCGAATAACCCCACAAGGAACGCCCGCCGCCCCGACGGTTTGTGGGATGt                                                                                                  >  1:1015261/1‑141 (MQ=255)
                                          cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGGGAATAAACCGCCAAGGAACCCCCGCCGCCACGACGGTTGGGGGGATGTc                                                                                                 >  1:236860/1‑141 (MQ=255)
                                              gCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCCGCCGCCACGCCGGTTGGTGGGATGTCtttt                                                                                             >  1:1717751/1‑141 (MQ=255)
                                                gTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTGGCGAATAAACCGACAAGGAACGCCAGCCCCCCCGCCGGTTGGTGGAATTTCTTTtgt                                                                                           >  1:927323/1‑141 (MQ=255)
                                                gTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAAAAAACCCACAAAGAAAGCCCCCCCCCCCCACGGTTTGTGGGAATTATTTtgt                                                                                           >  1:929384/1‑141 (MQ=255)
                                                 tATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtgtg                                                                                          <  1:1855342/141‑1 (MQ=255)
                                                  accAGGCCATTCGTATACTTTCACCCGACGATTCAACTTTATACTCTTTGACATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAGAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                         <  1:1980773/139‑1 (MQ=255)
                                                  aTCTGGCAATTTTTATAATTTATCCTTACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                         <  1:2123886/141‑1 (MQ=255)
                                                  aTCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                         <  1:949121/141‑1 (MQ=255)
                                                  aTCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                         <  1:1110553/141‑1 (MQ=255)
                                                  aTCAGGCAATTTTTATAAATTAACCAGACCATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGGCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                         <  1:1505102/141‑1 (MQ=255)
                                                        cAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGAtac                                                                                   <  2:2229483/141‑1 (MQ=255)
                                                          aTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAAGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTTTGACGAtacta                                                                                 >  1:1159636/1‑141 (MQ=255)
                                                          aTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACACGCACAGGGACGCCCGCCGCCCCCCAGGGTGGTGGTATGTCTTTTGTGTCGAtacta                                                                                 >  1:958463/1‑141 (MQ=255)
                                                                gtaATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGTAAAACCCGACAAGGAACGCCTGCCGGCACCACGTGTGGGGGAATGTCTTTTGTGGGGAGACAACAcgcg                                                                           >  2:1511765/2‑141 (MQ=255)
                                                                ataATTTAATCTGACGAATAAACTTAATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGGGAATAAGCAGAAAAGGAAAGCACGCCGCCACGACGGGTTGTTGAATGTCTTTTGTTACGATACACCCcgcg                                                                           >  2:547273/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCGACAAGGAACGCCACCCGCCACCACGTTTGGTGGAATGTCTTTTTTGACGATACTACCcccg                                                                           >  1:2197692/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGCCAACGCACGCCAGCCGCCACGACGGTTTGGGGTATGTCTTTTGTGTCGTTACTAACcgcg                                                                           >  1:588970/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGTCGTTTGGTGGAATGTCTTTTGTGTCGCTACAACCcgcg                                                                           >  2:1182462/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTGTTTTGTGACGATACTACCcgcg                                                                           >  2:114212/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCcgcg                                                                           >  2:1563387/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCcgcg                                                                           >  2:816959/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCcgcg                                                                           >  2:2375261/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGAAGATACAACCcgcg                                                                           >  2:70689/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCCACCACGAACCCCCCCCGCCACGACCTTTTGTGGAATGTCTTTTTTGAAGATAAAACCcgcc                                                                           >  1:112993/1‑140 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGCCAGCCGCCACGACCGTTGGTGGTATGTCTTTTGTGACGATACTACCcgcg                                                                           >  2:2376005/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCcgcg                                                                           >  2:568984/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGTATGTCTTTTGTGACGATACTACCcgcg                                                                           >  2:883457/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGTCTTTTGTGAAGATACAACCcgcg                                                                           >  2:1856174/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAAAGCCAGACGCCACCACGGTTGGTGGAAAGTCGTTTGTGACGATAATAACcgcg                                                                           >  2:1497773/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAAACCAGAATGAACGCCAGCCGCCACGAAGATTTGTGGAATGTGTTTTGTGAAGATAAGACCcccg                                                                           >  2:1934382/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCCACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATTTCTTTTGTGACGATACTACCcgcg                                                                           >  2:1693119/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCCACAAGGAACGCCAGCCCCCACGACGTTTGGTGTAATGTCTTTTGTGACGATACAAcccccc                                                                           >  2:1357722/1‑138 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCCCGAAAAAACCGACAATGAACGCCAGCCGCCACGACCTTTGGTGGAATGAGATTAGTGACGATACTACCcgcg                                                                           >  2:547277/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGCAAAAACCCACAAAGAACGCCACCCGCCCCCACGGTTTGTGGGATTTCTTTTGTGTCGATACAAACcgcg                                                                           >  1:2197707/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCCCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACAAACcgcc                                                                           >  2:377601/1‑140 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAAAGCCAGCCGCCACGACGGTTGGGGGTATGTCTTTTGTGTCGATAATACCcccg                                                                           >  2:113968/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGGATGTCTTTTGTTACGATTCTAACcgcg                                                                           >  2:2456155/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACGCCAGCCCCCACCACGGTTGGTGGTATGTCTTTTTTGACGATAATAACcgcg                                                                           >  1:135134/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCCCAAGGAACGCCCGCCGCCACCACGGTTGGGGGGATGTGTTTTGTTTCGATACTAACcccg                                                                           >  2:879302/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAAAGCCAGCCGCCACCACGTTTTGTGGGATGTCTTTTGTGTCGATAATACCcccg                                                                           >  1:1720189/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAAACCGACAACGAAAGCCCCCCGCCACCACGGTTTGTGGAATTTCTTTTTTGACGATACTACCcccg                                                                           >  1:1913957/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAAACCCACAAGGAACCCCAGCCGCCCCGACGGTTGGTGGTATGTCTTTTTTGACGTAAATACCcgcg                                                                           >  2:70000/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTCGCGAAAAACCCCCCAAGGAACCCCCGCCGCCACCACCGTTGGTGGCAAGTCTTTTGTGACGATACAAcccccc                                                                           >  1:1542340/1‑138 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGCGTATAAACCCACAAGGAACGCCACCCCCCCCGACGGTTGGTGGTATGTGGTTTGTGACGATAATACCcgcg                                                                           >  1:135028/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGCGAATAACCCGACAAGGAACGCCACCCGCCACGACGGTTGGTGGAAAGTCTTTTGTGACGATACTACCcccg                                                                           >  2:8334/1‑141 (MQ=255)
                                                                ataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTGGCGAATAAACCCACAAGGAACCCCCGCCGCCACCACGGTTTGTGGGATGTGTTTTGTGACGATACAACCcccg                                                                           >  1:274783/1‑141 (MQ=255)
                                                                 taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTGGCGGATAACCCCACAATGAAAGCCAGCCCCCACGTCGGTTGGTGGTATGTATTTTGTGTCGATACTACCCgcgc                                                                          >  1:2521531/1‑141 (MQ=255)
                                                                 taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTGGCGAAAAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGTCTTTTGTGACGTTACTACCCccgc                                                                          >  2:1563179/1‑141 (MQ=255)
                                                                 taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTAcccccac                                                                          >  2:1635080/1‑137 (MQ=255)
                                                                 taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACGCCACCCGCCACGACGTTTGGTGGAATTTGTTTTGTGTCGATACTACCcgcac                                                                          >  2:2219775/1‑139 (MQ=255)
                                                                 taATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGCATGTGTTTTGTGACGATACAACCCccgc                                                                          >  2:1998718/1‑141 (MQ=255)
                                                                   aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCa                                                                        <  2:2180296/141‑1 (MQ=255)
                                                                                      cTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAGAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                                     <  2:1475708/141‑1 (MQ=255)
                                                                                           tactCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAGAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTg                                                <  2:2262382/138‑1 (MQ=255)
                                                                                                tttGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGcc                                           <  1:260020/141‑1 (MQ=255)
                                                                                                  tGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTg                                         <  1:1814313/141‑1 (MQ=255)
                                                                                                       taataGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACCACGTTTGGTGGAATGTATTTTTTGACGATACTACCCGCGCCAAAAACAGAATTATACCCGATGGTGACGCCAGGAAta                                    >  2:1792143/1‑141 (MQ=255)
                                                                                                          taGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCTCGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaata                                 <  2:828480/141‑1 (MQ=255)
                                                                                                             tGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAAcc                              <  2:331751/141‑1 (MQ=255)
                                                                                                              gCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAACCCCACCCCCCACGAAGTTTGGTGGAATGTCTTTTTTTTCGAAACAAACCCCGCCAAAAAAAGAATTATCCCCCATGGTGAAGCCTGGATTAATAACca                             >  2:830724/1‑141 (MQ=255)
                                                                                                                  aTCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATg                         <  2:240048/141‑1 (MQ=255)
                                                                                                                  aTCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATg                         <  2:2131869/141‑1 (MQ=255)
                                                                                                                   tCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGa                        <  1:2375261/141‑1 (MQ=255)
                                                                                                                   tCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGa                        <  2:102861/141‑1 (MQ=255)
                                                                                                                   tCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGa                        <  2:1231961/141‑1 (MQ=255)
                                                                                                                   tCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGa                        <  1:2380889/141‑1 (MQ=255)
                                                                                                                      cGGTCGTTTTTTTAGCGAATAACCCCACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTATTTTGTGAAGATAAAACCCGCGCCAAAAACATAATTATACCCGATGGTTAAGCCAGGGTTAATAACCACAttaaat                     >  2:1814215/1‑136 (MQ=255)
                                                                                                                        gTCGTTTATTTCGCGATTAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc                   <  2:1443170/141‑1 (MQ=255)
                                                                                                                        gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc                   <  1:377601/141‑1 (MQ=255)
                                                                                                                        gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc                   <  2:1307890/141‑1 (MQ=255)
                                                                                                                        gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc                   <  1:506609/141‑1 (MQ=255)
                                                                                                                        gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACCTcc                   <  1:377598/141‑1 (MQ=255)
                                                                                                                        gTCGTTTATTTCGCGAATAACCCGAAAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc                   <  2:581492/141‑1 (MQ=255)
                                                                                                                               aTTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTCTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAg            <  2:912224/141‑1 (MQ=255)
                                                                                                                                     cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa      <  2:772537/141‑1 (MQ=255)
                                                                                                                                     cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa      <  1:2291915/141‑1 (MQ=255)
                                                                                                                                     cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTa      <  1:2291954/141‑1 (MQ=255)
                                                                                                                                         tAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGc  >  2:2254740/1‑141 (MQ=255)
                                                                                                                                         tAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGCGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTACTGc  >  2:1063538/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
GATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGC  >  NC_000913/361180‑361457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑