Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,726,996 | 0 | A | . | 72.7% | ‑6.9 / 10.7 | 11 | noncoding (2189/2904 nt) | rrlG | 23S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base A (3/0); new base . (2/6); total (5/6) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTC > NC_000913/2726918‑2727092 | ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:1824037/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 2:1762988/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 2:488763/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCGACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:1323600/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCGACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACACGTTTCAAAGcc > 1:1377357/4‑141 (MQ=1) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:1694141/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:1868888/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:627189/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:627260/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACAGATACTGGCGTACCTGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:1552474/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACAGATACTGGCGTACCTGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:1554195/138‑1 (MQ=0) | TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTC > NC_000913/2726918‑2727092 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |