Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,833 C→G R197G (CGT→GGT)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,8330CG81.3% 378.0 / 80.6 139R197G (CGT→GGT) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (2/24);  new base G (31/82);  total (33/106)
Fisher's exact test for biased strand distribution p-value = 3.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 142 total aligned reads displayed.

GGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTG  >  NC_000913/4181704‑4181970
                                                                                                                                 |                                                                                                                                         
    tcttctTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGcc                                                                                                                            <  2:286094/141‑1 (MQ=255)
    tcttctTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGcc                                                                                                                            >  2:2033736/1‑141 (MQ=255)
    tcttctTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGcc                                                                                                                            >  2:1702837/1‑141 (MQ=255)
    acctcattGACTCCCACAACGGTAACACCCACGCTCCGGGTAAAGGGCACAACACCGCGCGTATCATCCCGTACCGTGCGTCCTGGCTGGACTTCGAATGCGATCCCAAGGACAACCAGTTCGTACGAATCGACCGTCGcc                                                                                                                            <  2:121473/135‑1 (MQ=255)
         tttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTaaa                                                                                                                       >  1:725865/1‑141 (MQ=255)
          ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAAc                                                                                                                      >  2:2273981/1‑141 (MQ=255)
          ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAAc                                                                                                                      >  2:1580936/1‑141 (MQ=255)
          ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAAc                                                                                                                      <  1:1984123/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACTGCGTGTTCGTGCGTATCGACCGGCGCCGCAAActgccggcc                                                                                                              <  1:2014254/141‑2 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  2:1368244/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:1937270/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:2296489/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:2483827/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:676965/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:900086/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:928668/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  1:1431637/141‑1 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCg                                                                                                              <  2:1567425/141‑1 (MQ=255)
                    cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGAc                                                                                                            <  2:1493672/141‑1 (MQ=255)
                     aaaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGAcc                                                                                                           <  2:899693/141‑1 (MQ=255)
                      aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACca                                                                                                          <  1:1006781/141‑1 (MQ=255)
                      aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACca                                                                                                          <  1:1006553/141‑1 (MQ=255)
                      aaGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACca                                                                                                          <  1:1007074/141‑1 (MQ=255)
                       aGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACcat                                                                                                         <  1:1862296/141‑1 (MQ=255)
                         gTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACcatca                                                                                                       <  2:1516752/141‑1 (MQ=255)
                           aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCAtt                                                                                                     <  2:477511/141‑1 (MQ=255)
                                ccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcg                                                                                                <  1:1124853/141‑1 (MQ=255)
                                 cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgc                                                                                               <  1:218937/141‑1 (MQ=255)
                                 cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgc                                                                                               <  1:218953/141‑1 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  2:613181/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  2:309301/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  2:694724/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  1:653631/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  2:1275145/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  2:1560390/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCAATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                              >  1:1960553/1‑141 (MQ=255)
                                   ctctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACCTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcgc                                                                                             <  1:1839367/141‑1 (MQ=255)
                                     ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                           <  1:245914/141‑1 (MQ=255)
                                     ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                           <  1:483750/141‑1 (MQ=255)
                                        cGGGTAACGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGAACTTCATTCTGCGCGCCGTGa                                                                                        <  2:934356/141‑1 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                        <  2:934304/141‑1 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                        <  2:887199/141‑1 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                        <  1:453004/141‑1 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                        <  2:1959182/141‑1 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                        <  2:1959191/141‑1 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCAGCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGa                                                                                        <  2:269136/141‑1 (MQ=255)
                                             aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCAGAACTac                                                                                   <  2:2509810/141‑1 (MQ=255)
                                             aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                                                                   <  2:2473834/141‑1 (MQ=255)
                                               aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTacac                                                                                 <  1:123030/141‑1 (MQ=255)
                                                  gCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                              <  1:1544473/141‑1 (MQ=255)
                                                  gCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                              <  1:1222393/141‑1 (MQ=255)
                                                       ataACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGc                                                                         <  2:225597/141‑1 (MQ=255)
                                                        taACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCa                                                                        <  1:2052023/141‑1 (MQ=255)
                                                        taACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCa                                                                        <  2:722548/141‑1 (MQ=255)
                                                        taACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACATCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCa                                                                        <  1:2052324/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                       <  1:817741/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                       <  2:1623996/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                       <  2:1178668/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                       <  1:313404/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                       <  2:2176985/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                       <  2:1915868/141‑1 (MQ=255)
                                                          aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                      >  1:314106/1‑141 (MQ=255)
                                                          aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                      >  2:2093192/1‑141 (MQ=255)
                                                           cgcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGAt                                                                     <  2:1801327/141‑1 (MQ=255)
                                                            gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATc                                                                    >  2:2030188/1‑141 (MQ=255)
                                                               cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                 >  1:1711951/1‑141 (MQ=255)
                                                               cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                 >  1:1711745/1‑141 (MQ=255)
                                                               cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                 >  1:1711887/1‑141 (MQ=255)
                                                               cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                 >  1:1485289/1‑141 (MQ=255)
                                                                        ccTTCCCGCGCTTCCTGGCTGGACTTCGCATTCGATCCGAAGGACAACCTGTTCGTCGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:1115090/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:811759/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:654498/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:552315/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:346841/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:654514/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:128322/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:1416655/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:698648/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:698723/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:2258132/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  1:2377192/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  1:1835010/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCAGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:530310/141‑1 (MQ=255)
                                                                        ccTTACCGCGGTTCCTGGCTGGACTTCGAGTTCGACCCGAAGGACTGCGTGTTCGTGCGTATCGACCGGCGCCGCAAACTGCCGGCCTCGGTGCTGCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                        <  2:2432943/141‑1 (MQ=255)
                                                                             ccGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGa                                                   <  1:2461566/141‑1 (MQ=255)
                                                                                 ggTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaaa                                               >  1:1022378/1‑141 (MQ=255)
                                                                                 ggTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGaaaaa                                               >  1:1022389/1‑141 (MQ=255)
                                                                                       tggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATc                                         >  2:1159889/1‑141 (MQ=255)
                                                                                            ggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCTCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTAGa                                    >  1:1773158/1‑141 (MQ=255)
                                                                                                    aaTTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTg                            <  1:2035296/141‑1 (MQ=255)
                                                                                                           tCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACaa                     <  2:794647/141‑1 (MQ=255)
                                                                                                               acggACAACCTGTTCGTAGGTATCGACCGTCGCCGTACACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                 <  2:568852/139‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                 <  2:79874/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCTACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                 <  2:2324657/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                 <  1:1394541/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTACGCAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCAACATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                 <  2:568853/141‑1 (MQ=255)
                                                                                                                aGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGc                <  1:1852791/141‑1 (MQ=255)
                                                                                                                            tCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTgg    <  2:1965758/141‑1 (MQ=255)
                                                                                                                              gtaggtaTCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTg  <  2:2174707/141‑1 (MQ=255)
                                                                                                                              gtaggtaTCGACCGTCGCCGTAAACTGCCTGCGACAATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTg  <  2:1368717/141‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                         
GGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTG  >  NC_000913/4181704‑4181970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: