BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	939,935	G→A	A170T (GCT→ACT)	serS →	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	939,935	0	G	A	75.2%	281.3 / 102.7	149	A170T (GCT→ACT)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (6/31); new base A (22/90); total (28/121)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGG  >  NC_000913/939796‑940071

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCTGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  2:706240/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  2:1075886/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  1:710855/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  1:1237459/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  1:20333/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  1:984627/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  2:43572/141‑1 (MQ=255)

cAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGc                                                                                                                                         <  1:1557826/141‑1 (MQ=255)

       tGGGGTACCCCGCGTGATTTTGTCTTTGAAGTTCGTGACCACGTGACGCTTGGTGAAATTCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTTGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATg                                                                                                                                  <  2:1515938/141‑1 (MQ=255)

       tGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATg                                                                                                                                  <  2:182816/141‑1 (MQ=255)

       tGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATg                                                                                                                                  <  2:1810660/141‑1 (MQ=255)

       tGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATg                                                                                                                                  <  2:281785/141‑1 (MQ=255)

       tGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATg                                                                                                                                  <  2:738492/141‑1 (MQ=255)

          ggTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCAc                                                                                                                               >  1:2567735/1‑141 (MQ=255)

             aCCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACcgc                                                                                                                            >  2:1986407/1‑141 (MQ=255)

               cccGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCgcgc                                                                                                                          <  2:1658355/141‑1 (MQ=255)

                ccGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCa                                                                                                                         >  1:1233339/1‑141 (MQ=255)

                ccGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCa                                                                                                                         >  1:1234122/1‑141 (MQ=255)

                  gcgTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACt                                                                                                                       >  1:728656/1‑141 (MQ=255)

                      gAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCg                                                                                                                   <  2:216021/141‑1 (MQ=255)

                      gAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCg                                                                                                                   <  1:1762711/141‑1 (MQ=255)

                      gAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCg                                                                                                                   <  1:1762300/141‑1 (MQ=255)

                       aGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGc                                                                                                                  <  1:211992/141‑1 (MQ=255)

                            gACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGttt                                                                                                             <  2:2458033/141‑1 (MQ=255)

                            gACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGttt                                                                                                             <  2:1834829/141‑1 (MQ=255)

                            gACTATGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTAAAGCTGACTGGTTCCCGCATTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGttt                                                                                                             <  2:1293783/141‑1 (MQ=255)

                                ttGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGc                                                                                                         >  1:331411/1‑141 (MQ=255)

                                 tGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCt                                                                                                        <  2:776028/141‑1 (MQ=255)

                                 tGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCt                                                                                                        <  2:776210/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGACATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGGTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:2274824/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:1063331/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:1717211/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:1106941/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:1450151/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:1308757/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:1024272/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:1973726/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:300201/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:300195/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:2499928/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:2202871/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:387461/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:397909/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  2:585789/141‑1 (MQ=255)

                                   aaGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTgg                                                                                                      <  1:1286427/141‑1 (MQ=255)

                                        cGTGACCACGTGACGCTGGGTGACATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTg                                                                                                 <  2:697679/141‑1 (MQ=255)

                                        cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTg                                                                                                 <  2:147986/141‑1 (MQ=255)

                                        cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTg                                                                                                 <  2:906891/141‑1 (MQ=255)

                                        cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTg                                                                                                 <  2:906892/141‑1 (MQ=255)

                                        cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTg                                                                                                 <  1:2026046/141‑1 (MQ=255)

                                        cGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTg                                                                                                 <  1:919034/141‑1 (MQ=255)

                                          tGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACGTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCa                                                                                               >  2:1688780/1‑141 (MQ=255)

                                             ccACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATAc                                                                                            <  2:1848480/141‑1 (MQ=255)

                                             ccACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATAc                                                                                            <  1:1139542/141‑1 (MQ=255)

                                                cGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATGCCGa                                                                                         <  1:1136612/141‑1 (MQ=255)

                                                cGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGa                                                                                         <  1:326338/141‑1 (MQ=255)

                                                  tGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAAc                                                                                       <  1:908715/141‑1 (MQ=255)

                                                     cGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGc                                                                                    <  2:1899876/141‑1 (MQ=255)

                                                       cTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCAt                                                                                  <  1:2441680/141‑1 (MQ=255)

                                                       cTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCAt                                                                                  <  2:68356/141‑1 (MQ=255)

                                                       cTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCAt                                                                                  <  1:2042391/141‑1 (MQ=255)

                                                         gggTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATgg                                                                                >  2:2070296/1‑141 (MQ=255)

                                                         gggTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATgg                                                                                >  2:2070310/1‑141 (MQ=255)

                                                              aaaTGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACa                                                                           <  2:1750352/141‑1 (MQ=255)

                                                                 tGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTg                                                                        <  1:986701/141‑1 (MQ=255)

                                                                   cACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTgag                                                                      <  1:2191039/141‑1 (MQ=255)

                                                                   cACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTgag                                                                      >  1:575226/1‑141 (MQ=255)

                                                                   cACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTgag                                                                      >  2:2197435/1‑141 (MQ=255)

                                                                   cACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTgag                                                                      >  2:1681223/1‑141 (MQ=255)

                                                                   cACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTgag                                                                      <  2:1041944/141‑1 (MQ=255)

                                                                    aCTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTgaga                                                                     <  2:1282504/141‑1 (MQ=255)

                                                                     ctctGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGaa                                                                    <  2:1744520/141‑1 (MQ=255)

                                                                            cTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGtt                                                             >  1:521265/1‑141 (MQ=255)

                                                                                  tttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGTTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                       <  1:1502113/141‑1 (MQ=255)

                                                                                  tttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                       <  2:1730925/141‑1 (MQ=255)

                                                                                  tttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                       <  2:1730457/141‑1 (MQ=255)

                                                                                  tttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                       <  2:1727811/141‑1 (MQ=255)

                                                                                  tttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTAGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                       <  1:622962/141‑1 (MQ=255)

                                                                                  tttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAc                                                       <  1:2326299/141‑1 (MQ=255)

                                                                                   ttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAcc                                                      >  1:511781/1‑141 (MQ=255)

                                                                                   ttGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTAcc                                                      >  1:1155723/1‑141 (MQ=255)

                                                                                     gCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTg                                                    <  1:634903/141‑1 (MQ=255)

                                                                                           gCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAAc                                              <  2:357883/141‑1 (MQ=255)

                                                                                           gCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAAc                                              <  1:573460/141‑1 (MQ=255)

                                                                                                tAAGCTGACTGGGTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGa                                         <  2:152198/141‑1 (MQ=255)

                                                                                                     tGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGTGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGc                                    <  1:2454434/141‑1 (MQ=255)

                                                                                                     tGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGc                                    <  1:1652751/141‑1 (MQ=255)

                                                                                                     tGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGc                                    <  2:572352/141‑1 (MQ=255)

                                                                                                     tGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGc                                    <  2:1242101/141‑1 (MQ=255)

                                                                                                     tGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGc                                    <  1:1335287/141‑1 (MQ=255)

                                                                                                        cTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTgt                                 >  1:1263211/1‑141 (MQ=255)

                                                                                                        cTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTgt                                 <  1:737852/141‑1 (MQ=255)

                                                                                                                cGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTgtacggtacg                         >  2:2277762/1‑141 (MQ=255)

                                                                                                                cGCTTTGTGGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTgtacggtacg                         >  2:1790261/1‑141 (MQ=255)

                                                                                                                     tgtgGTAATGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCa                    <  2:11187/141‑1 (MQ=255)

                                                                                                                           aaTGAAAGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGcc              >  1:1302281/1‑141 (MQ=255)

                                                                                                                                aaGGGCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAAt         >  2:166891/1‑141 (MQ=255)

                                                                                                                                  gggCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAAttt       <  1:149617/141‑1 (MQ=255)

                                                                                                                                    gCAGATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGc     <  2:589838/141‑1 (MQ=255)

                                                                                                                                       gATTACTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTgg  <  1:75651/141‑1 (MQ=255)

CAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTGGTTCCCGCTTTGTGGTAATGAAAGGGCAGATTGCTCGCATGCACCGCGCACTGTCGCAGTTTATGCTGGATCTGCATACCGAACAGCATGGCTACAGTGAGAACTATGTTCCGTACCTGGTTAACCAGGACACGCTGTACGGTACGGGTCAACTGCCGAAATTTGCTGG  >  NC_000913/939796‑940071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`