Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,562,251 G→A L47L (TTG→TTA yffO → CPZ‑55 prophage; uncharacterized protein YffO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,562,2510GA84.0% 870.9 / 137.1 339L47L (TTG→TTAyffOCPZ‑55 prophage; uncharacterized protein YffO
Reads supporting (aligned to +/- strand):  ref base G (22/32);  new base A (59/225);  total (82/257)
Fisher's exact test for biased strand distribution p-value = 2.83e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 341 total aligned reads displayed.

AAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGAT  >  NC_000913/2562115‑2562391
                                                                                                                                        |                                                                                                                                            
aaGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCa                                                                                                                                          >  1:591247/1‑141 (MQ=255)
  ggCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCTAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAg                                                                                                                                        <  2:1409198/141‑1 (MQ=255)
  ggCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAg                                                                                                                                        <  2:746990/141‑1 (MQ=255)
  ggCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAg                                                                                                                                        <  2:747130/141‑1 (MQ=255)
     gAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATc                                                                                                                                     <  1:1357153/141‑1 (MQ=255)
          gATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCat                                                                                                                                <  1:346303/141‑1 (MQ=255)
          gATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCat                                                                                                                                <  1:2380188/141‑1 (MQ=255)
           aTAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCatg                                                                                                                               <  1:299166/141‑1 (MQ=255)
           aTAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCatg                                                                                                                               <  2:1010693/141‑1 (MQ=255)
            tAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAATAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCatga                                                                                                                              >  1:1619181/1‑141 (MQ=255)
            tAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCACATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCACGATCAGCatga                                                                                                                              >  1:2123901/1‑141 (MQ=255)
                  aCAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAg                                                                                                                        >  1:1895798/1‑141 (MQ=255)
                   cAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTCAGCGTAGGTTATAGTTTACGCAAGATCAGCATGATGCCAgg                                                                                                                       <  2:548069/141‑1 (MQ=255)
                   cAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAgg                                                                                                                       <  2:2059790/141‑1 (MQ=255)
                   cAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAgg                                                                                                                       <  2:1497921/141‑1 (MQ=255)
                   cAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAgg                                                                                                                       <  2:791576/141‑1 (MQ=255)
                   cAATGGGTTAGACAATTATGACACCAGCGAAACACCGAAAAAGCGCGGACGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAgg                                                                                                                       <  2:77095/141‑1 (MQ=255)
                    aaTTGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAATGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:856857/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:1632395/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:357856/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:617500/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:26120/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:238226/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:196336/141‑1 (MQ=255)
                    aaTGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      >  1:1480478/1‑141 (MQ=255)
                    aaTGGGATAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:1363476/141‑1 (MQ=255)
                    aaTGGGAGAGACATTGATGACACCAGCGAACCACCGAACACGCGCGGTCGGCCAGCAAAATATACTGCAAAAATAGCCACGCAAATCGTCCAATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAggg                                                                                                                      <  2:2316343/141‑1 (MQ=255)
                              aCATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGcc                                                                                                            <  1:1056129/141‑1 (MQ=255)
                                aTTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCAc                                                                                                          <  1:929888/141‑1 (MQ=255)
                                aTTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCAc                                                                                                          <  1:2060971/141‑1 (MQ=255)
                                 tttATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCAcc                                                                                                         >  1:2310405/1‑141 (MQ=255)
                                  ttATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCg                                                                                                        <  2:2014797/141‑1 (MQ=255)
                                  ttATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCg                                                                                                        <  2:1800751/141‑1 (MQ=255)
                                  ttATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCg                                                                                                        <  2:94339/141‑1 (MQ=255)
                                   taagccacCCGCGAAACCCCGCACAAGCGCGGTCGGCCAGCTAACTATAATGACACAATAGCAACGCAAATCGTCCTATTGGTAAGCGTAGTTTATAGGTTAGGCAAGCTCAGCATGATGCAAGGGATGCCGAGCCACCGt                                                                                                       <  2:1871566/136‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGACAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:1165066/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:2091508/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:1240149/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:2137585/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:736318/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:1473757/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:2392527/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:2998/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:384312/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:796951/141‑1 (MQ=255)
                                   tATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGt                                                                                                       <  2:333265/141‑1 (MQ=255)
                                     tGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCa                                                                                                     <  2:134361/141‑1 (MQ=255)
                                     tGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCa                                                                                                     <  2:833033/141‑1 (MQ=255)
                                     tGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCa                                                                                                     <  2:2180298/141‑1 (MQ=255)
                                                 aaCACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTg                                                                                         <  2:1545490/141‑1 (MQ=255)
                                                  acacCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTgg                                                                                        >  2:193550/1‑141 (MQ=255)
                                                    acCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCa                                                                                      >  1:227251/1‑141 (MQ=255)
                                                     ccGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCaa                                                                                     >  1:1263264/1‑141 (MQ=255)
                                                     ccGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCaa                                                                                     >  1:1963006/1‑141 (MQ=255)
                                                             gcgcGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCa                                                                             <  2:1779643/141‑1 (MQ=255)
                                                               gcgGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCata                                                                           >  2:2454244/1‑141 (MQ=255)
                                                                  gTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATgg                                                                        <  1:1829658/141‑1 (MQ=255)
                                                                  gTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATgg                                                                        <  2:1295102/141‑1 (MQ=255)
                                                                      gCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACtt                                                                    <  2:2413390/141‑1 (MQ=255)
                                                                        catcTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:2114375/138‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:2425491/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:2225506/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:63888/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:900502/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:1210915/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:1836519/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:2002197/141‑1 (MQ=255)
                                                                        cAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCa                                                                  <  2:2259245/141‑1 (MQ=255)
                                                                             aaaTATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCagggaa                                                             <  2:2543389/141‑1 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGCAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGCGCCACCGTCAGATGTTGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  2:2205494/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  1:1034191/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  1:1127826/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCCGGGAAGGGATAg                                                      >  2:672023/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  2:1911254/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  1:1536748/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  1:615670/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  2:1727947/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  2:445478/1‑141 (MQ=255)
                                                                                    aTGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAg                                                      >  2:538095/1‑141 (MQ=255)
                                                                                     tGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGc                                                     <  2:960592/141‑1 (MQ=255)
                                                                                       aaaaaaTAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCt                                                   <  2:1760586/141‑1 (MQ=255)
                                                                                         aaaaTAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGCGCATATGGACTTCAGGGAAGGGATAGACTgg                                                 >  1:897417/1‑141 (MQ=255)
                                                                                              aGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTc                                            <  1:492269/141‑1 (MQ=255)
                                                                                                       aaTCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATg                                   >  1:649805/1‑141 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCTGTGTATTTATATCCTGGATGTCGTGTCTATTGt                                 <  2:2349999/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:602278/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:44749/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  1:77229/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGATATGGt                                 <  1:2466787/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:824478/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:970436/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:2507717/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:2331764/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:1815576/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:1191362/141‑1 (MQ=255)
                                                                                                         tCGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGt                                 <  2:1382120/141‑1 (MQ=255)
                                                                                                          cGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTg                                <  1:552490/141‑1 (MQ=255)
                                                                                                          cGTCCTATTGGTAAGCGAAGGTTATAGTTTACGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTg                                <  2:1077473/141‑1 (MQ=255)
                                                                                                                                       tGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCgaga   >  1:377365/1‑141 (MQ=255)
                                                                                                                                        gcgcAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGAt  >  1:2229064/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                            
AAGGCGAACTGATAGAAAACAATGGGTTAGACATTTATGACACCAGCGAAACACCGAAAAAGCGCGGTCGGCCAGCTAAATATAATGAAAAAATAGCAACGCAAATCGTCCTATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGAT  >  NC_000913/2562115‑2562391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: