Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,7270GT27.6% 176.3 / 12.6 98P169T (CCG→ACG) yadLputative fimbrial protein YadL
Reads supporting (aligned to +/- strand):  ref base G (41/30);  new base T (0/27);  total (41/57)
Fisher's exact test for biased strand distribution p-value = 1.83e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTG  >  NC_000913/151588‑151867
                                                                                                                                           |                                                                                                                                            
aGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGa                                                                                                                                             >  2:377560/1‑141 (MQ=255)
 gTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                                                            >  2:811848/1‑141 (MQ=255)
 gTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                                                            >  2:811697/1‑141 (MQ=255)
    ggATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGagaat                                                                                                                                         >  2:728444/1‑141 (MQ=255)
         cATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACACGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCTTGCACTCATCGGATAATAAACg                                                                                                                                    >  2:545130/1‑141 (MQ=255)
          atatatAGTTCCTGAATCAGTAAGTCTGTTAGTTATATGATCCTGTACATGTGCCTGGTGCAGAAAACTAACCCGCAGTGACATAGTTTACGGTTTTATTTTTTACCACGACCAGGCGTGCACTCATCGGATAATAATCGa                                                                                                                                   >  2:1031685/1‑141 (MQ=255)
                aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAt                                                                                                                             >  1:1333098/1‑141 (MQ=255)
                 gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt                                                                                                                            >  1:1077408/1‑141 (MQ=255)
                 gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt                                                                                                                            >  1:46810/1‑141 (MQ=255)
                 gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt                                                                                                                            >  2:766616/1‑141 (MQ=255)
                 gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt                                                                                                                            >  1:1637609/1‑141 (MQ=255)
                 gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt                                                                                                                            >  1:1425603/1‑141 (MQ=255)
                  ttCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTg                                                                                                                           >  2:118960/1‑141 (MQ=255)
                   tCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTgg                                                                                                                          >  1:61014/1‑141 (MQ=255)
                      tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTg                                                                                                                       >  2:1936177/1‑141 (MQ=255)
                           cTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTg                                                                                                                  >  1:1375656/1‑141 (MQ=255)
                             ttAATTCTGTTATTTTTAGGTTACTTTAATTGTGGCTGGGGCAGAAAAATTCCCCGCAGTTACTTTGTTTAGGGTTTTATTTTTTTCCACGACCCGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGga                                                                                                                <  2:201750/140‑1 (MQ=255)
                              tAAGTCTTTTATTTATTGGTTACTTTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACTTTGTTTCCGGTTTTTTTTTTTTCCACGACCCGGGTTGCACTCATGGTATAAAACACGACCGCATTGGCTGCAGTGgag                                                                                                               <  1:1545966/141‑1 (MQ=255)
                                  tCTTTTAGTTATAGGTTACTGTAATTGTGGCTGGTGCATAAAACTTACCCGCAGTGACATTTTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTAATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTg                                                                                                           <  2:1428936/141‑1 (MQ=255)
                                    ttttatttatTGTTTACTTTAATTGTGGCTGGTCCACAAAACTCACCCGCTTTGACTTTTTTTAGGGTTTTATTTTTTCCCCCCACCAGGCGTCCCCACATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGtt                                                                                                         <  1:287651/139‑1 (MQ=255)
                                    ttttagttaTCGGTTACTGTAATTGGGGCTGGTGCATAAAACTTACCCGCAGTGAAATTTTTTACGGTTTTATTTTTTCCCCCGCCCTGGCGTGCACTCTTCGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGtt                                                                                                         <  1:287597/139‑1 (MQ=255)
                                     tttctttaTCGGTTACTTTAAATGTGGCTGGTGCAGAAAACTTACCCGCCGTGACATTGTTTAGGGTTTTTTTTTTTTCCACGACCAGGCGTGCACTCATGGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                                                        <  2:297553/136‑1 (MQ=255)
                                     tttatttatCGGTTACTTTAATTGTGGCTGGTGCATAAAACTTACCCGCCTTGAAATTTTTTCGGGTTTTATTTTTTCCCACCACCAGGCGTGCACTCATCGTAAAAAAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                                                        <  1:1162822/140‑1 (MQ=255)
                                     gttcgtaaTAGGACAGTGAAAATGTGTCTGTGGCAGAAAACTTACCCGCTGTCACATTGTATACGGTATATTTGATTTCCACCACCTGGCTTGCCCCCACGGTATAATAAACGCCCGCCTTGGCTGCAGTGGAGATTGTTa                                                                                                        <  2:297562/141‑1 (MQ=255)
                                     gttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTTTTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                                                        <  2:545129/141‑1 (MQ=255)
                                     gttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                                                        >  1:1158636/1‑141 (MQ=255)
                                       tttttaTTGGTTACTTTAATTGTGGCTGGTGCATAAAACTAACCCGCTGTTACTTTTTTTAGGTTTTTTTTTTTTTCCCCGACCTGGCGTGCACTCTTGGTAAAAAAAACGACCGCATTGGCTGCAGTGGAGATTGTTAcc                                                                                                      <  1:1097463/138‑1 (MQ=255)
                                       tatttaTAGGTTACTTAAAAGGTGGCTGTTCCAGAAAACTTACCCGCATGGACATTTTTTAGGGTTTTATTTTTTACCCCGCCCGGGCTTGCAATCATCTGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTAcc                                                                                                      <  1:1097423/138‑1 (MQ=255)
                                        ttttttcggTTACTTTAATTGTGGCGGGTGAATAAAACTTACCCGCCGTGACTTTGTTTAGGGTTTTTTTTTTTCCCCCGACCTGGCGGGCACTCAGCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACct                                                                                                     <  1:630383/134‑1 (MQ=255)
                                           tataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                                                  >  1:1921932/1‑141 (MQ=255)
                                           tataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                                                  >  1:632384/1‑141 (MQ=255)
                                               ggTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGa                                                                                              >  2:793939/1‑141 (MQ=255)
                                               ggTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGa                                                                                              >  2:793982/1‑141 (MQ=255)
                                               ggTTACTGTAAATGTGGCTGGTGCAGAAAAATTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGGGCACTCATCGGATAATAAACGACCGCAATGGCTGCAGTGGAGATTGGTCCCGCTTgaga                                                                                              >  2:510199/1‑141 (MQ=255)
                                                 ttACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATg                                                                                            >  1:1896388/1‑141 (MQ=255)
                                                 ttACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATg                                                                                            >  1:1369753/1‑141 (MQ=255)
                                                   attttAATTGTGGCTGTTTCACAAAAATAACCCCTTTTGACTTTTTTTCGGGTTTTATTTTTTTAAACTCCCTGGGGTGCACACTTCGGTTAAAAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          <  1:1442323/137‑1 (MQ=255)
                                                   acttaaaaTGGGGCTGGTCAATAAAACTAACCCGCTGTTACATTGTTTACGTTTTTATTTTTTACCCCGACCCGGCTTGCACAAATGGTAAAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          <  1:673687/136‑1 (MQ=255)
                                                   aCTGTATTTGTGGCTGGTGCAGAAAACTTCCCCGCCGTGACATTTTTTACGTTTTTTTTTTTTGCCACGACCAGGCGTGCACTCATGGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          <  1:1597302/141‑1 (MQ=255)
                                                   aCTGTAATTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACTTTGTTTACGGTTTTATTTTTTTCCCCCACCAGGCGTGCACTCATGGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          <  1:1442280/141‑1 (MQ=255)
                                                   aCTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCACATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          <  1:1299350/141‑1 (MQ=255)
                                                   aCTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          >  2:1649786/1‑141 (MQ=255)
                                                   aCTGTAAATGTGGCGGGTGAATAAAACTTACCCGAAGTGACATTGTTTCGGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                          <  1:1374669/141‑1 (MQ=255)
                                                           tgtgGCTGGTGCATAAAACTTACCCGCAGTGACATTTTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTac                                                                                  <  2:895046/141‑1 (MQ=255)
                                                           tgtgGCTGGTGCAGAAAACTTACCCGCTGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTac                                                                                  <  2:895000/141‑1 (MQ=255)
                                                             tgGCTGGTGCAGAAAACTTACCCGCAGTTAATTTTTTTACGTTTTTTTTTTTTTCCACGACCCGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                <  1:422850/141‑1 (MQ=255)
                                                             tgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCTGGCGTCCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                <  2:29249/141‑1 (MQ=255)
                                                             tgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                <  2:1768705/141‑1 (MQ=255)
                                                             gggCTGTTGCATAAAACTTACCCGCAGTTAATTTTTTTAGGGTTTTATTTTTTACCACGACCAGGCGTGCACCCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                <  1:6962/140‑1 (MQ=255)
                                                              ggCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGTTTTTTTTTTTTACCACGACCAGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                                               <  1:1828353/141‑1 (MQ=255)
                                                              ggCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                                               >  2:1307358/1‑141 (MQ=255)
                                                              ggCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                                               >  2:825546/1‑141 (MQ=255)
                                                              ggCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                                               >  2:267856/1‑141 (MQ=255)
                                                              ggCTGGTGAATAAAAATTACCCGCAGTGACTTTGTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACACTTCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                                               <  1:429424/141‑1 (MQ=255)
                                                                  ggTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTa                                                                           >  1:1096503/1‑141 (MQ=255)
                                                                  ggTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTa                                                                           >  1:833766/1‑141 (MQ=255)
                                                                     gCATAAAACTTACCCGCTGTGACTTTTTTTACGTTTTTATTTTTTTCCACGACCCGGCGTCCACTCATGGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAt                                                                        <  1:1011833/141‑1 (MQ=255)
                                                                     ccacaaaaCTCACCCGCTGTGACATTGTTTACGTTTTTATTTTTTCCCCCGCCCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAt                                                                        <  1:1068619/137‑1 (MQ=255)
                                                                            aCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTg                                                                 >  2:664353/1‑141 (MQ=255)
                                                                               tccccGCAGTGAAATTTTTTACGGTTTTATTTTTTTCCACGCCCAGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCAc                                                              <  1:955544/139‑1 (MQ=255)
                                                                                  ccGCTGTGACATTGTTTACGGTTTTTTTTTTTACCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                                                           <  2:1983889/141‑1 (MQ=255)
                                                                                      aGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGt                                                       >  2:726043/1‑141 (MQ=255)
                                                                                      aGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGt                                                       >  2:726044/1‑141 (MQ=255)
                                                                                        tGACTTTGTTTCCGGTTTTATTTTTTCCCCCGACCCGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTg                                                     <  2:1716437/141‑1 (MQ=255)
                                                                                          actttttttACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                                   <  1:340386/135‑1 (MQ=255)
                                                                                          actttGTTTACGGTTTTATTTATTACCACGACCAGGCGTGCACACCTCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                                   <  1:853550/138‑1 (MQ=255)
                                                                                          aaaTTTTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATGGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                                   <  1:1950354/139‑1 (MQ=255)
                                                                                          aaaTTGTTTCCGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                                   <  2:490049/139‑1 (MQ=255)
                                                                                          aaaTTGTTTACGTTTTTTTTTTTTTCCACGACCTGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                                   <  1:1275293/139‑1 (MQ=255)
                                                                                          aCATTGTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                                   <  2:83757/141‑1 (MQ=255)
                                                                                             ttGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                                                <  2:1105453/141‑1 (MQ=255)
                                                                                                 ttACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTa                                            >  1:1716601/1‑141 (MQ=255)
                                                                                                  tccGGTTTTATTTTTTTCCCCGACCAGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTAc                                           <  1:1242485/139‑1 (MQ=255)
                                                                                                  tACGTTTTTATTTTTTTCCACTACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTAc                                           <  2:888107/141‑1 (MQ=255)
                                                                                                          tATTTTTTCCCCCGACCAGGGGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTcc                                   <  1:1766696/141‑1 (MQ=255)
                                                                                                          tATTTTTTCCCCCGACCAGGCGTGCACTCATCGGTTAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTcc                                   <  1:1504357/141‑1 (MQ=255)
                                                                                                          tATTTTTTACCCCGACCCGGGTTGACCTAATGGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTcc                                   <  1:1338632/141‑1 (MQ=255)
                                                                                                                tttCCACGACCTGGGGTGAACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACtt                             <  1:1040387/141‑1 (MQ=255)
                                                                                                                tttCCACGACCAGGCGTCCACTCAGCGTATAAAAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACtt                             <  1:1040233/141‑1 (MQ=255)
                                                                                                                    cccGACCCGGCGTGCACTCATGGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAAc                         <  1:155761/139‑1 (MQ=255)
                                                                                                                     aCGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACg                        <  2:358969/141‑1 (MQ=255)
                                                                                                                          cAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGAc                   <  2:1991829/141‑1 (MQ=255)
                                                                                                                               gttcaatCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCAc              <  1:1139513/135‑1 (MQ=255)
                                                                                                                               gtccACTCAGCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCAc              <  1:209235/138‑1 (MQ=255)
                                                                                                                               gTGCACTCATCGTATAATAATCGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCAc              <  2:941678/141‑1 (MQ=255)
                                                                                                                               gTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCAc              <  1:407110/141‑1 (MQ=255)
                                                                                                                               gTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCAc              <  1:1139486/141‑1 (MQ=255)
                                                                                                                               gTGCACTCAGCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCAc              <  1:109428/141‑1 (MQ=255)
                                                                                                                                tGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACt             >  2:896925/1‑141 (MQ=255)
                                                                                                                                tGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACt             >  2:897189/1‑141 (MQ=255)
                                                                                                                                     tattCGGATAAACAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCtt        <  1:1167175/138‑1 (MQ=255)
                                                                                                                                     tCATGGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCtt        <  1:1955670/141‑1 (MQ=255)
                                                                                                                                     tCATGGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCtt        <  1:973649/141‑1 (MQ=255)
                                                                                                                                      cATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCttt       >  1:347335/1‑141 (MQ=255)
                                                                                                                                      cATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCttt       >  1:347334/1‑141 (MQ=255)
                                                                                                                                      cATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCttt       >  1:1586242/1‑141 (MQ=255)
                                                                                                                                      cATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCttt       >  1:1586238/1‑141 (MQ=255)
                                                                                                                                      cATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCttt       >  2:1252402/1‑141 (MQ=255)
                                                                                                                                           gATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTg  >  2:1516368/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTG  >  NC_000913/151588‑151867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: