Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,6130GT30.9% 98.7 / 11.0 68Q155K (CAA→AAA) malFmaltose ABC transporter membrane subunit MalF
Reads supporting (aligned to +/- strand):  ref base G (31/16);  new base T (21/0);  total (52/16)
Fisher's exact test for biased strand distribution p-value = 1.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAAC  >  NC_000913/4244485‑4244752
                                                                                                                                |                                                                                                                                           
catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTGAGTTGCAGTTTTTGCTCGCCGCCcaa                                                                                                                                 >  1:176379/1‑141 (MQ=255)
catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTAAGTTGCAGTTTTTTCTCGCCGCCaaa                                                                                                                                 >  1:209975/1‑141 (MQ=255)
catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCcaa                                                                                                                                 >  1:1130954/1‑141 (MQ=255)
catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCaaa                                                                                                                                 >  2:532433/1‑141 (MQ=255)
catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAATTGCAGTTTTTTCTCCCCGCCaaa                                                                                                                                 >  1:209991/1‑141 (MQ=255)
catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTGACTTGCAGGTTTTTCTCGCCGCCcaa                                                                                                                                 >  1:602375/1‑141 (MQ=255)
 atcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTCAGTTTTTTCTCGCCGCCCAAt                                                                                                                                >  1:82486/1‑141 (MQ=255)
   caCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCAAAttt                                                                                                                              >  2:153871/1‑141 (MQ=255)
      tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaa                                                                                                                           <  1:570695/141‑1 (MQ=255)
      tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaa                                                                                                                           <  1:570686/141‑1 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                          >  2:1484786/1‑141 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                          >  1:753452/1‑141 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCAAATTTaaaa                                                                                                                          >  2:692508/1‑141 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                          >  2:974564/1‑141 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTGAGTTGCAGTTTTTGCTCGCCGCCCAATTTaaaa                                                                                                                          >  1:273360/1‑141 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTTTTTGCTCGCCGCCCAATTTaaaa                                                                                                                          >  2:55549/1‑141 (MQ=255)
       ttgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                          >  1:961846/1‑141 (MQ=255)
              cATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGGAGTTTTTTCTCGCCGCCAAAATTAATAGCGTCgg                                                                                                                   >  1:1847224/1‑141 (MQ=255)
                                      cACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGATTCAATTGCAGTTTTTTGTCGCCGCCCAATTTTAAAGAGTCGGCGATGTAATTTTTTGCGGTTTAGc                                                                                           >  1:1694866/1‑141 (MQ=255)
                                         tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTGAGTTGCAGTTTTTTCTCGCCGCCAAATTTAAAATCGTCGGAGAGGTAATTTTTTCCGGTTTCGCCGt                                                                                        >  2:1618085/1‑141 (MQ=255)
                                         tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCCGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGt                                                                                        >  2:480986/1‑141 (MQ=255)
                                         tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGt                                                                                        >  2:104455/1‑141 (MQ=255)
                                         tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGGTGCAGGTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGt                                                                                        >  2:363292/1‑141 (MQ=255)
                                         tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTGCAGTTTTTGGGCGCCGCCCAATTTAAAAGCGTCGGAGTGGTAATTTTTTCCGGTTTCGCCgg                                                                                        >  1:681749/1‑140 (MQ=255)
                                         tCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCGTTCAGTTGCAGGTTTTGCTCGCCGCCCAATTTAAAAGAGTCGGAGCGGTAATTTTTGACGGTTTGGcccg                                                                                        >  1:1052907/1‑139 (MQ=255)
                                                   aCGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAgcgc                                                                              <  2:408528/141‑1 (MQ=255)
                                                     gATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCa                                                                            <  1:105229/141‑1 (MQ=255)
                                                     gATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCa                                                                            <  1:104577/141‑1 (MQ=255)
                                                     gATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCa                                                                            <  1:105172/141‑1 (MQ=255)
                                                      aTTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAATTTTGCCGGTTTCGCCGTCGCTGAGCGCCAg                                                                           >  2:1764331/1‑141 (MQ=255)
                                                      aTTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTTCGTTGGAGTTTTTGCTCGCCGCCCAATTTAAAATCGTCGGAGAGGTAAATTTTGCCGGTTTCGCCGGCGCGGAGCGCCAg                                                                           >  1:300332/1‑141 (MQ=255)
                                                       ttCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGt                                                                          <  1:501672/141‑1 (MQ=255)
                                                         cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAATCGTCGGAGAGGTAATTTTTTCCGGTTTCGCCGTCGCTGAGCGCCAGTTg                                                                        >  2:1141219/1‑141 (MQ=255)
                                                         cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTTCCGGTTTCGCCGTCGCTGAGCGCCAGTTg                                                                        >  2:1141234/1‑141 (MQ=255)
                                                         cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTGAGTTTGACTTTTTTCTCGCCGCCAAATTTAAAAACGGCGGAGAGGTAAATTTTTGCGGGTTGGCCGTCGCGGTGCGCCAGGTg                                                                        >  1:1674924/1‑141 (MQ=255)
                                                                aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTTCAGTTTTTTCTCGCCGCCCAAAATAAAAGCGTAGGCGAGGTAATTTTTGGCGGTTTGGCCCTCGCTGAGCGCCAGTTGCcacaca                                                                 >  1:522220/1‑141 (MQ=255)
                                                                aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTTTTTGCTCGCCCCCAAATTTAAAAGCCTCGGAGAGGTAATTTTTTCCGGGTTCGCCGTCGCTGAGCGCCCGTTGCcacaca                                                                 >  1:1211509/1‑141 (MQ=255)
                                                                aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTAAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTAGCCGTCGCTGAGCGCCAGTTGCcacaca                                                                 >  1:1977776/1‑141 (MQ=255)
                                                                   aCGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcg                                                              >  2:1459186/1‑141 (MQ=255)
                                                                     gcgcAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCCAATTTAAAATCGTCGGAGAGGTAATTTTTTCCGGGTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcc                                                            >  1:315453/1‑141 (MQ=255)
                                                                              cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTGAGTTTGAGGTTTTTGTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTGGCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCCCACATAGCCCGCCGGGTa                                                   >  1:528782/1‑141 (MQ=255)
                                                                                  cgTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTTCTCGGCGCCACAATTAAAATCGTCGGAGTGGTTATTTTTGGCGGTTTAGCCGTCGCTGAGCCCCCGTTTCCACTCAACGCCCGCCGGGTAAAGa                                               >  1:1306545/1‑141 (MQ=255)
                                                                                  cgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTAGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTGTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCCCCGGGTAAAGa                                               >  2:1762214/1‑141 (MQ=255)
                                                                                  cgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTGCAGTTTTTTCTGGCCGCCAAATTTAAAATCGGAGGAGAGGTAATTTTTTGCGGTTTCGCCCTCGCTGAGCGCCAGTTTCCACACATAGCCCGCCCGGGAAAGa                                               >  1:610404/1‑141 (MQ=255)
                                                                                  cgTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAGTTTGAGTTTTTTCTCGCCGCCAAAATTAAAAACGTCGGAGAGGTAATTTTTTCCGGTTTAGCCGTCGCTGTGCGCCAGTTTGCACACAAAGCCCGCCGGGTaaata                                               >  1:312449/1‑139 (MQ=255)
                                                                                  cgTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTTGAGTTTTTGGGCGCCGCCAAATTTAAAAACGTCGGAGAGGTAATTTTTTGCGGTTTCGCCGTCGCTGTGCGCCACTTGCCCCGCAAAGCCCGCCCGGTaaata                                               >  1:194464/1‑139 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTTTGTTTCCGTTGCAGTTTTTGCACGCCGCCAAATTTAAAATCGTCGGATAGGTAATTTTTTCGGGGTTCGCCGTCCCAGAGGGCCATATGCCACTCATAGCCCGCCGGGTAAAGAcc                                             >  2:229321/1‑141 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTGTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                             >  2:1409707/1‑141 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTTTGTTTAAGTTGGAGTTTTTTCTCGCCGCCAAAATTAAAATCGTCGGAGAGGTAATTTTTTCCGGTTTAGCCGTCGCGGAGCGCCACTTGCCACACAACGCCCCCCCGGTaaaaaac                                             >  1:2026179/1‑137 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTCAGTTTTTTCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTGGCGGGTTCGCCGTCGCTGATCGCCAGTTGCCACACATCGCCCGCCCGGTAAAGaac                                             >  1:1420441/1‑139 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                             >  2:1267334/1‑141 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAATTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTTCCACACATAGCCCGCCGGGTAAAGAcc                                             >  2:1909986/1‑141 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTGTGTTTGAGTTGCAGTTTTTTGTCGCCGCCCAAATTAAAAACGTGGGGGAGGTAATTTTTTGCGGTTTAGCCGTCGCTGAGCGCCCGTTGCCACTCAAAGCCCCCCGGGGAAAAAcc                                             >  1:99760/1‑141 (MQ=255)
                                                                                    ttCGCCTTCGGGCTGGGCGGTCGTGTGTTTAAGTTTCAGTTTTTTGTCGCCCCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTGGCGGTTTAGCCGTCGCTGAGCGCCAGATTCCACAAATAGCCCGCCCGGTaaataaa                                             >  1:1420478/1‑137 (MQ=255)
                                                                                      cGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGTGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaa                                           <  1:2001202/141‑1 (MQ=255)
                                                                                      cGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaa                                           <  2:665837/141‑1 (MQ=255)
                                                                                      cGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaa                                           <  2:665537/141‑1 (MQ=255)
                                                                                       gCCTTCGGGCTGGGCGGTCGTTTCTTTCTGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGTGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGACACTCATCGCCCGCCGGGTAAAGACCaaa                                          <  2:1489944/141‑1 (MQ=255)
                                                                                            cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTtat                                     <  2:775519/141‑1 (MQ=255)
                                                                                                    gCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTg                             <  1:1410912/141‑1 (MQ=255)
                                                                                                       gTCGTTTGTTTCAGTTGCAGGTTTTTCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCAACGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTTCCt                          >  2:1015601/1‑141 (MQ=255)
                                                                                                       gTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCt                          <  1:104455/141‑1 (MQ=255)
                                                                                                         cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGc                        <  2:1955162/141‑1 (MQ=255)
                                                                                                                 tcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAATACCAAAGTTATAGGTTTTGCCTGCTTGCCAgg                >  1:873577/1‑141 (MQ=255)
                                                                                                                   aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAg              >  1:918207/1‑141 (MQ=255)
                                                                                                                   aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAg              >  2:1762890/1‑141 (MQ=255)
                                                                                                                   aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAg              >  2:1465370/1‑141 (MQ=255)
                                                                                                                               tGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaac  <  1:153871/141‑1 (MQ=255)
                                                                                                                                |                                                                                                                                           
CATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAAC  >  NC_000913/4244485‑4244752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: