Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,727,004 | 0 | A | C | 45.5% | ‑6.4 / 10.0 | 12 | noncoding (2181/2904 nt) | rrlG | 23S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base A (1/5); new base C (4/1); total (6/6) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.01e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT > NC_000913/2726918‑2727103 | ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:577623/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:577626/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 1:580174/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGC‑CAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc > 2:673449/4‑141 (MQ=0) ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc > 1:540994/4‑141 (MQ=1) gtaaaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGTCCATCAAACATTG‑TAGGGTGGGAGTTCCACGTCGGGTCCGTGCAGTCTGGCGTCCCGACGTCAAAGCCTCCCACCTATCCTACACAt > 2:1239728/4‑141 (MQ=1) cccccaGACACTGTCCGCAACCCGGATTACGGGACAACGTTAGAACATCAAACAATA‑AAGGGTGGTATATCAAGCTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTCTCCTACACATCAAGGCTCaa < 2:1711987/138‑1 (MQ=1) aggcacTGTCCGCCACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGATCCACGAATACTGGCGTACTCGTTTCAAAGACTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:261962/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 1:1731306/138‑1 (MQ=0) aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATC‑AACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc < 2:321142/138‑1 (MQ=0) tggccGCAACCCGCATTACGGGTCAACGTTAGAACATCCAACATTA‑AAGGCTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCAACAATTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTc < 2:234193/138‑1 (MQ=1) gccaCCCGGATTACGGGTCAACGTTAGAACAACACACATTA‑CAGGGAGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCCTCCCACCTATACTACACATCAAGGCTCAATGTTCAGTGTCAAGCt < 2:1359324/138‑1 (MQ=1) | TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTA‑AAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT > NC_000913/2726918‑2727103 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |