Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0040AC45.5% ‑6.4 / 10.0 12noncoding (2181/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (1/5);  new base C (4/1);  total (6/6)
Fisher's exact test for biased strand distribution p-value = 8.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT  >  NC_000913/2726918‑2727103
                                                                                       |                                                                                                   
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                               >  1:577623/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                               >  1:577626/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                               >  1:580174/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                               >  2:673449/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                               >  1:540994/4‑141 (MQ=1)
                   gtaaaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGTCCATCAAACATTGTAGGGTGGGAGTTCCACGTCGGGTCCGTGCAGTCTGGCGTCCCGACGTCAAAGCCTCCCACCTATCCTACACAt                            >  2:1239728/4‑141 (MQ=1)
                             cccccaGACACTGTCCGCAACCCGGATTACGGGACAACGTTAGAACATCAAACAATAAAGGGTGGTATATCAAGCTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTCTCCTACACATCAAGGCTCaa                  <  2:1711987/138‑1 (MQ=1)
                                  aggcacTGTCCGCCACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGATCCACGAATACTGGCGTACTCGTTTCAAAGACTCCCACCTATCCTACACATGTAGGGTCAATGTTc             <  1:261962/138‑1 (MQ=0)
                                  aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc             <  1:1731306/138‑1 (MQ=0)
                                  aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc             <  2:321142/138‑1 (MQ=0)
                                        tggccGCAACCCGCATTACGGGTCAACGTTAGAACATCCAACATTAAAGGCTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCAACAATTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTc       <  2:234193/138‑1 (MQ=1)
                                             gccaCCCGGATTACGGGTCAACGTTAGAACAACACACATTACAGGGAGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCCTCCCACCTATACTACACATCAAGGCTCAATGTTCAGTGTCAAGCt  <  2:1359324/138‑1 (MQ=1)
                                                                                       |                                                                                                   
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCT  >  NC_000913/2726918‑2727103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: