Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,727,0350GA45.5% ‑5.1 / 10.4 15noncoding (2150/2904 nt)rrlG23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base A (5/0);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCAC  >  NC_000913/2726918‑2727158
                                                                                                                      |                                                                                                                           
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                      >  1:577623/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                      >  1:577626/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                      >  1:580174/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCATACACTGTCCTCGATCCGGATAACGGACCTGAGTTAGAACCTCAAAGTTGCCAGGGTGGTATTTCAAGGATGGCTCCATGAGAACTGGCGTCCCCACTTCAAAGcc                                                                                                      >  2:673449/4‑141 (MQ=0)
ttgggaggaGACCGCCCCAGTCAAACTACCCACCAGGCACTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGcc                                                                                                      >  1:540994/4‑141 (MQ=1)
                   gtaaaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGTCCATCAAACATTGTAGGGTGGGAGTTCCACGTCGGGTCCGTGCAGTCTGGCGTCCCGACGTCAAAGCCTCCCACCTATCCTACACAt                                                                                   >  2:1239728/4‑141 (MQ=1)
                             cccccaGACACTGTCCGCAACCCGGATTACGGGACAACGTTAGAACATCAAACAATAAAGGGTGGTATATCAAGCTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTCTCCTACACATCAAGGCTCaa                                                                         <  2:1711987/138‑1 (MQ=1)
                                  aggcacTGTCCGCCACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGATCCACGAATACTGGCGTACTCGTTTCAAAGACTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                    <  1:261962/138‑1 (MQ=0)
                                  aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                    <  1:1731306/138‑1 (MQ=0)
                                  aggcacTGTCCGCAACCCCGATAAGGGGTCAACGTTAGAACATCAACACTACAAGGGTGGTATTTCAAGGACGGCTCCACGAATACTGGCGTACTCGTTTCAAAGCCTCCCACCTATCCTACACATGTAGGGTCAATGTTc                                                                    <  2:321142/138‑1 (MQ=0)
                                        tggccGCAACCCGCATTACGGGTCAACGTTAGAACATCCAACATTAAAGGCTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCAACAATTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTc                                                              <  2:234193/138‑1 (MQ=1)
                                             gccaCCCGGATTACGGGTCAACGTTAGAACAACACACATTACAGGGAGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTACAAAGCCTCCCACCTATACTACACATCAAGGCTCAATGTTCAGTGTCAAGCt                                                         <  2:1359324/138‑1 (MQ=1)
                                                                                      aacgggTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGt                 <  2:1347760/138‑1 (MQ=1)
                                                                                                     aaggatggCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTcac  <  1:227338/135‑1 (MQ=1)
                                                                                                     aaggatggCTCCATGAGAACTGGCGTCCCCACTTCAAAGCCTCCCACCTATCCTACACAAGCAAATTCAAAGTCCAGTGCAAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTAGCCGCGGATACACTGCATCTTcac  >  2:2116209/7‑141 (MQ=0)
                                                                                                                      |                                                                                                                           
TTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCAC  >  NC_000913/2726918‑2727158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: