| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,037,308 | 0 | T | A | 37.5% | ‑5.5 / 25.9 | 24 | noncoding (49/76 nt) | alaT | tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base T (9/6); new base A (6/3); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACT > NC_000913/4037178‑4037439 | ttagagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGAt > 1:685297‑M1/83‑141 (MQ=255) gagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATccc < 1:2019618‑M1/62‑1 (MQ=255) gagcgcacccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATccc < 1:1098393‑M1/62‑1 (MQ=255) ccctgataagggtgaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCATAGCt > 2:1439943‑M1/72‑141 (MQ=255) gaggtcggcagttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGcttggctccaccactttcgc > 2:793747‑M1/59‑134 (MQ=255) gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa > 2:1093202‑M1/49‑141 (MQ=255) gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa > 2:1093207‑M1/49‑141 (MQ=255) gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa > 2:1093246‑M1/49‑141 (MQ=255) gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa > 2:1093341‑M1/49‑141 (MQ=255) gttcaaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaa > 2:1093201‑M1/49‑141 (MQ=255) caaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaataa > 2:356099‑M1/46‑141 (MQ=255) caaatctgcccagacctaccattacatggttcagccgtagaatacGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATtaaataa > 2:2152620‑M1/46‑141 (MQ=255) tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa > 2:1013856‑M1/42‑141 (MQ=255) tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa > 2:36977‑M1/42‑141 (MQ=255) tctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCTGCCTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATaaaaaa > 2:231712‑M1/42‑141 (MQ=255) atgtGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATaaaaaa < 2:1783632/138‑1 (MQ=1) atgtGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATaaaaaa < 2:496222/138‑1 (MQ=1) atgtGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATaaaaaa < 2:1678113/138‑1 (MQ=1) atgtGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATaaaaaa < 2:1237302/138‑1 (MQ=1) ctgcccagacctaccattacatggttcagccgtagaatacGGGGCCATAGCTCAGCTGGGAGAGCGCCCGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAAt > 2:734623‑M1/41‑141 (MQ=255) ttggAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTa < 2:1481037/138‑1 (MQ=1) ttagAAGAGGATTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCATCTCTGTAGTGATTAAATCAAAAATACTTCAGAGTGTa < 2:428442/138‑1 (MQ=1) tcagacgagGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTGTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTa < 1:489901/135‑1 (MQ=0) aatgacgTCTGCGGTTCGATCCCGCATAGATCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACt > 2:670852/7‑141 (MQ=0) | AAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGATTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTCACTGCGAAGTTTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACT > NC_000913/4037178‑4037439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |