Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,843 G→C R200P (CGT→CCT)  rpoB → RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,8430GC85.3% 482.9 / 68.5 156R200P (CGT→CCT) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (3/20);  new base C (38/95);  total (41/115)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 156 total aligned reads displayed.

GGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGC  >  NC_000913/4181704‑4181979
                                                                                                                                           |                                                                                                                                        
    tcttctTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGcc                                                                                                                                     <  2:1852211/141‑1 (MQ=255)
    tcttctTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGcc                                                                                                                                     <  2:1328311/141‑1 (MQ=255)
     cttcttTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCCTCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCg                                                                                                                                    >  1:779760/1‑141 (MQ=255)
      ttcttTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGt                                                                                                                                   <  1:1929672/141‑1 (MQ=255)
          ttGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAAc                                                                                                                               >  1:2106835/1‑141 (MQ=255)
                 cGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAActgcctgc                                                                                                                        >  1:567086/1‑141 (MQ=255)
                 cGACAAAGGTAAAACCCACTCTTAGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAActgcctgc                                                                                                                        >  2:1057920/1‑141 (MQ=255)
                  gACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCg                                                                                                                       <  2:883188/141‑1 (MQ=255)
                    cAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGAc                                                                                                                     <  2:2136875/141‑1 (MQ=255)
                           aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTCCCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCAtt                                                                                                              <  2:886319/141‑1 (MQ=255)
                           aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCAtt                                                                                                              <  2:1121247/141‑1 (MQ=255)
                           aaaaCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCAtt                                                                                                              <  2:886357/141‑1 (MQ=255)
                                ccACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcg                                                                                                         <  1:151790/141‑1 (MQ=255)
                                 cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc                                                                                                        <  2:2005199/141‑1 (MQ=255)
                                 cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc                                                                                                        <  2:1298005/141‑1 (MQ=255)
                                 cACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgc                                                                                                        <  2:1297925/141‑1 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                       >  1:594356/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                       >  2:1372623/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                       >  2:1630603/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                       >  2:1344945/1‑141 (MQ=255)
                                  aCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcg                                                                                                       >  2:1656815/1‑141 (MQ=255)
                                   ctctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTgcgcgc                                                                                                      <  1:1852611/141‑1 (MQ=255)
                                     ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                    <  1:1816483/141‑1 (MQ=255)
                                     ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                    <  2:229067/141‑1 (MQ=255)
                                     ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                    <  2:1747453/141‑1 (MQ=255)
                                     ctTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGccc                                                                                                    <  2:1747461/141‑1 (MQ=255)
                                       tCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTg                                                                                                  >  1:1439237/1‑141 (MQ=255)
                                        cGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTACGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCAGTCTGCGCGCCCTGa                                                                                                 <  2:268712/141‑1 (MQ=255)
                                            tAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTa                                                                                             <  2:1308187/141‑1 (MQ=255)
                                            tAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGATGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTa                                                                                             <  2:147259/141‑1 (MQ=255)
                                             aaaGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTac                                                                                            <  1:530296/141‑1 (MQ=255)
                                               aGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAGCTGCCTGCGACCATCATTCTGCGCGCCCTGAACTacac                                                                                          <  1:1747479/141‑1 (MQ=255)
                                                  gCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTAcaccac                                                                                       <  1:150972/141‑1 (MQ=255)
                                                     gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACaga                                                                                    >  1:1048312/1‑141 (MQ=255)
                                                     gTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACaga                                                                                    >  1:622666/1‑141 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:2005809/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:594356/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:1595566/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:1963376/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:1207168/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:12736/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:1489184/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:922845/141‑1 (MQ=255)
                                                         aaCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAg                                                                                <  2:2063694/141‑1 (MQ=255)
                                                          aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                               >  2:1803513/1‑141 (MQ=255)
                                                          aCGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                               >  2:597077/1‑141 (MQ=255)
                                                          aCGCGCGTATCATCACTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTTTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGa                                                                               >  2:597086/1‑141 (MQ=255)
                                                           cgcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCTGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGAt                                                                              <  2:298357/141‑1 (MQ=255)
                                                            gcgcgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATc                                                                             >  2:422017/1‑141 (MQ=255)
                                                               cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                          >  1:1299998/1‑141 (MQ=255)
                                                               cgTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTc                                                                          >  1:945556/1‑141 (MQ=255)
                                                                      tcCCTGACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGt                                                                   <  2:666311/141‑1 (MQ=255)
                                                                        cccgaCCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1283087/137‑1 (MQ=255)
                                                                        ccacaCCGTCGTTCCTGGCTGCACAACCAATTCGAACCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGAAAACTGCCTGCGACCATCATTCTGCTCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1196781/137‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1915182/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTAGGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  1:1351590/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1950507/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  1:1688672/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  1:1476042/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  1:1688825/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:277741/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:368756/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:951399/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:190891/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1904580/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  1:517425/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1022667/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  1:1386941/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1517321/141‑1 (MQ=255)
                                                                        ccTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1364109/141‑1 (MQ=255)
                                                                        ccTGACCGTGGGTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTc                                                                 <  2:1090360/141‑1 (MQ=255)
                                                                         cTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCt                                                                >  2:80830/1‑141 (MQ=255)
                                                                         cTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCt                                                                >  2:769491/1‑141 (MQ=255)
                                                                           tACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCttt                                                              >  1:1330964/1‑141 (MQ=255)
                                                                                      ctggctggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTAt                                                   >  2:745381/1‑141 (MQ=255)
                                                                                            ggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTTCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGa                                             >  2:569063/1‑141 (MQ=255)
                                                                                            ggACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGa                                             >  2:932842/1‑141 (MQ=255)
                                                                                                   gAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGt                                      <  2:1087480/141‑1 (MQ=255)
                                                                                                   gAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGt                                      <  1:886725/141‑1 (MQ=255)
                                                                                                   gAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGt                                      <  2:1890621/141‑1 (MQ=255)
                                                                                                      ttCGATCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGAt                                   <  2:899470/141‑1 (MQ=255)
                                                                                                           tCCGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACaa                              <  1:90321/141‑1 (MQ=255)
                                                                                                             cGAAGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAGGTTATCTTTGAAATCCGTGATAACAAGc                            >  2:2155996/1‑141 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:380291/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:1863292/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:667819/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:1206954/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:1559458/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:1915170/141‑1 (MQ=255)
                                                                                                               aaGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGGTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTg                          <  2:1914626/141‑1 (MQ=255)
                                                                                                                aGGACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGc                         <  1:1965145/141‑1 (MQ=255)
                                                                                                                  gACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAg                       <  2:1593873/141‑1 (MQ=255)
                                                                                                                  gACAACCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAg                       <  2:1603934/141‑1 (MQ=255)
                                                                                                                      aCCTGTTCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATgg                   <  2:1489435/141‑1 (MQ=255)
                                                                                                                           ttCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTg              >  1:1490543/1‑141 (MQ=255)
                                                                                                                            tCGTACGTATCGACCGTCGCCTTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTgg             <  1:1586099/141‑1 (MQ=255)
                                                                                                                            tCGTACGTATCGACCCTCGCCGTAAACTGCCTGCGACCATCAGTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTgg             <  1:40687/141‑1 (MQ=255)
                                                                                                                                    aTCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACCGGTGCCGGaa     >  2:1137682/1‑141 (MQ=255)
                                                                                                                                     tCGACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAAc    <  1:1212735/141‑1 (MQ=255)
                                                                                                                                       gACCCTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGc  >  1:1354841/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGTAAACTGCCTGCGACCATCATTCTGCGCGCCCTGAACTACACCACAGAGCAGATCCTCGACCTGTTCTTTGAAAAAGTTATCTTTGAAATCCGTGATAACAAGCTGCAGATGGAACTGGTGCCGGAACGC  >  NC_000913/4181704‑4181979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: