Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.

ALE, Flask, Isolate, Technical Replicate Clonal or Population ALE Sample Details Base Media Carbon Source(g/L) Nitrogen Source(g/L) Phosphorus Source(g/L) Sulfur Source(g/L) Calcium Source(g/L) Supplement(g/L) Temperature (Celsius) Strain Additional Strain Details Taxonomy ID Reference Genome
A0 F0 I1 R1 clonal None M9 None None None None None None 37 None None None NC_000913.3.gb
A4 F165 I0 R1 population ALE flasks alternate substrates. Post ALE enrichment with Glucose(4) M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A4 F165 I0 R2 population ALE flasks alternate substrates. Post ALE enrichment with Glycerol(2.5) M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A4 F165 I1 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A4 F165 I2 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A5 F160 I0 R1 population ALE flasks alternate substrates. Post ALE enrichment with Glucose(4) M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A5 F160 I0 R2 population ALE flasks alternate substrates. Post ALE enrichment with Glycerol(2.5) M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A5 F160 I1 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A5 F160 I2 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A5 F160 I3 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A6 F162 I0 R1 population ALE flasks alternate substrates. Post ALE enrichment with Glucose(4) M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A6 F162 I0 R2 population ALE flasks alternate substrates. Post ALE enrichment with Glycerol(2.5) M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A6 F162 I1 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb
A6 F162 I2 R1 clonal ALE flasks alternate substrates M9 None None None None None None 37 511145 BOP27 NC_000913_3.gb