MMV: Wirth_MMV668399_1 - Sample Meta Data
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Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.
| ALE, Flask, Isolate, Technical Replicate | Clonal or Population | ALE Sample Details | Base Media | Carbon Source(g/L) | Nitrogen Source(g/L) | Phosphorus Source(g/L) | Sulfur Source(g/L) | Calcium Source(g/L) | Supplement(g/L) | Temperature (Celsius) | Strain | Additional Strain Details | Taxonomy ID | Reference Genome |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A1 F1 I1 R1 | clonal | Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. | RPMI | Ca(NO3)2(4H2O)(0.1) | Na2HPO4(0.8) | MgSO4(0.04884) | None | ,MMV668399(high dose intermittent exposure) | 37 | 36329 | BMGF-Wirth-3D7-11-1-13 | Illumina/ | /var/data/GCF_000002765.5.gb | |
| A2 F1 I1 R1 | clonal | Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. | RPMI | Ca(NO3)2(4H2O)(0.1) | Na2HPO4(0.8) | MgSO4(0.04884) | None | ,MMV668399(high dose intermittent exposure) | 37 | 36329 | BMGF-Wirth-3D7-11-1-13 | Illumina/ | /var/data/GCF_000002765.5.gb | |
| A3 F1 I1 R1 | clonal | Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. | RPMI | Ca(NO3)2(4H2O)(0.1) | Na2HPO4(0.8) | MgSO4(0.04884) | None | ,MMV668399(high dose intermittent exposure) | 37 | 36329 | BMGF-Wirth-3D7-11-1-13 | Illumina/ | /var/data/GCF_000002765.5.gb | |
| A4 F1 I1 R1 | clonal | Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. | RPMI | Ca(NO3)2(4H2O)(0.1) | Na2HPO4(0.8) | MgSO4(0.04884) | None | ,MMV668399(high dose intermittent exposure) | 37 | 36329 | BMGF-Wirth-3D7-11-1-13 | Illumina/ | /var/data/GCF_000002765.5.gb | |
| A5 F1 I1 R1 | clonal | Further media components: sup:AlbumaxII(0.5%),sup:humanSerum(4.3%),sup:Hepes(25 mM),sup:NaHCO3(25 mM),sup:hypoxanthine(0.36 mM),antibiotic:gentamicin(100 µg/mL). Cultures were maintained in leukocyte-depleted red blood cells at 2.5% hematocrit. Compound-resistant parasites were developed using either a high-pressure intermittent (high dose intermittent), a step-wise, or a constant method of compound exposure. | RPMI | Ca(NO3)2(4H2O)(0.1) | Na2HPO4(0.8) | MgSO4(0.04884) | None | ,MMV668399(high dose intermittent exposure) | 37 | 36329 | BMGF-Wirth-3D7-11-1-13 | Illumina/ | /var/data/GCF_000002765.5.gb |