Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,366,751	Δ1,271 bp	IS5‑mediated	[yhcE]–[yhcG]	[yhcE], yhcF, [yhcG]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3365673–3366754	3368021	1268–2349	6 [5]	[0] 40	insH1–[yhcG]	insH1,yhcE,yhcF,[yhcG]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3366750	NA (NA)	40 (1.110)	36/254	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	3368022 =	0 (0.000)					coding (196/1128 nt)	yhcG	DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

ATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC  >  NC_000913/3365525‑3365695                                                                                                                                                    |                        aTCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGGGCGAATAAGCGGGGAAATTCCTCTCGGCTGACTCAGTCAATTCATTTCTTCATGTTTGCGCCGATATTTTCTCCCGTAAATGCATTGAATCTGCCTATTTAGACCGTttc                         >  7:148837/1‑148 (MQ=17)  tCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttctt                       >  1:183771/1‑149 (MQ=255)       gcagcaCAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCa                  <  3:149685/149‑1 (MQ=255)             caGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg            <  4:135872/149‑1 (MQ=255)               ggAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc          <  5:10882/149‑1 (MQ=40)                       aTCAATTAGGGAAGGTGCGAAGAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTGTGAGGCGAGTTTTTCTCCCGTAAATGCGTTGAATGAGCCTATTTAGCCCGTGTCTTCGCCATTTAAGGCGTTATccc  >  4:13332/1‑149 (MQ=11)                                                                                                                                                    |                        ATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC  >  NC_000913/3365525‑3365695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑