Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,366,751	Δ1,271 bp	IS5‑mediated	[yhcE]–[yhcG]	[yhcE], yhcF, [yhcG]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3365673–3366754	3368021	1268–2349	6 [5]	[0] 40	insH1–[yhcG]	insH1,yhcE,yhcF,[yhcG]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3366750	NA (NA)	40 (1.110)	36/254	0.2	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	3368022 =	0 (0.000)					coding (196/1128 nt)	yhcG	DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

TAGCGCCCGGACGGAAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACAC  >  NC_000913/3367898‑3368160                                                                                                                             |                                                                                                                                           gtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGAt                                                                                                                     <  5:115197‑M2/24‑1 (MQ=255)    tgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacgaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTAt                                                                                                                 >  1:105259‑M2/122‑149 (MQ=255)        ggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccc                                                                                                                                            <  8:134086‑M2/1‑1 (MQ=255)        ggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccc                                                                                                                                            >  7:134086‑M2/118‑118 (MQ=255)        ggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAgg                                                                                                                       >  4:6548‑M2/118‑139 (MQ=255)        ggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAgg                                                                                                                       <  3:6548‑M2/22‑1 (MQ=255)         gcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCt                                                                                                             >  3:22872‑M2/117‑148 (MQ=255)               actgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacgaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGt                                                                                                        <  2:105259‑M2/37‑1 (MQ=255)                     gaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGt                                                                                                >  7:134897‑M2/105‑149 (MQ=255)                        ttcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCaaa                                                                                                                         >  6:172912‑M2/102‑121 (MQ=255)                           actgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTa                                                                                                                  >  8:35435‑M2/99‑125 (MQ=255)                           actgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTa                                                                                                                  <  7:35435‑M2/27‑1 (MQ=255)                             tgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                                                                                        >  7:1409‑M2/97‑149 (MQ=255)                               tcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTtctc                                                                                        <  8:1409‑M2/53‑1 (MQ=255)                                                     tcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAg                                                                >  4:118055‑M2/73‑149 (MQ=255)                                                       atgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccc                                                                                                                                            >  1:23944‑M2/71‑71 (MQ=255)                                                       atgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccc                                                                                                                                            <  2:23944‑M2/1‑1 (MQ=255)                                                          atgacccctgttctatggctccagatgacaaacatgagctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCttt                                                           <  4:22872‑M2/82‑1 (MQ=255)                                                          atgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCttt                                                           >  6:128844‑M2/68‑149 (MQ=255)                                                               ccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTAc                                                      >  2:36757‑M2/63‑149 (MQ=255)                                                                      ctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCttt                                                <  8:134897‑M2/93‑1 (MQ=255)                                                                       tatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                                                                                          <  2:61191‑M2/35‑1 (MQ=255)                                                                       tatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAg                                                                                                          >  1:61191‑M2/55‑89 (MQ=255)                                                                        atggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCtttttt                                             >  7:23422‑M2/54‑149 (MQ=255)                                                                                    gacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCaa                                          <  7:105045‑M2/99‑1 (MQ=255)                                                                                    gacaaacatgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCaa                                          >  8:105045‑M2/42‑140 (MQ=255)                                                                                          catgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCAc                           >  5:2507‑M2/36‑149 (MQ=255)                                                                                          catgatctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCAc                           <  5:128844‑M2/114‑1 (MQ=255)                                                                                               tctcatatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGa                       >  2:146621‑M2/31‑148 (MQ=255)                                                                                                   atatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGa                       <  1:36757‑M2/118‑1 (MQ=255)                                                                                                   atatcagggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAAt                   <  1:146621‑M2/122‑1 (MQ=255)                                                                                                          ggacttgttcgcaccttccCTAATCAAGCGAGTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAAt                   >  4:14748‑M2/20‑141 (MQ=255)                                                                                                          ggacttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAAt                   <  3:14748‑M2/122‑1 (MQ=255)                                                                                                              ttgttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATtcatt                          >  4:3947‑M2/16‑128 (MQ=255)                                                                                                                gttcgcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTaa     >  7:121277‑M2/14‑149 (MQ=255)                                                                                                                    gcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCtt                                                 <  1:44929‑M2/92‑1 (MQ=255)                                                                                                                    gcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCtt                                                 >  2:44929‑M2/10‑101 (MQ=255)                                                                                                                    gcaccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAacac  >  1:176566‑M2/10‑148 (MQ=255)                                                                                                                     caccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGTGAAtt       <  8:101055‑M2/134‑1 (MQ=255)                                                                                                                     caccttccCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGTGAAtt       >  7:101055‑M2/9‑142 (MQ=255)                                                                                                                             |                                                                                                                                           TAGCGCCCGGACGGAAACGGTACGTAGCGTTAACGCGTTAATGACCGCGACGTACCAGGAAATTGGCCGACGAATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACAC  >  NC_000913/3367898‑3368160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑