Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 3,463,556 IS2 (+) +5 bp 35.4% coding (633‑637/984 nt) gspK → general secretory pathway component, cryptic

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)25 (0.290) 21/264 NT 31.6% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 3463560 52 (0.620)coding (637/984 nt) gspK general secretory pathway component, cryptic
* ? NC_000913 = 3187426NA (NA)32 (0.370) 27/264 NT 37.2% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 3463556 = 52 (0.620)coding (633/984 nt) gspK general secretory pathway component, cryptic

ATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186242‑3186096
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAGCATCTGATTGGCACTATGCCGGGGCGGCGTCTGGCTTTGGTA  <  NC_000913/3463560‑3463425
                                                                                                                                                                                                                                                                                           
ATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAC                                                                                                                                         >  8:320127/1‑148
ATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTAGTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGGCAGTCTACC                                                                                                                                        >  1:158234/1‑149
               GTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACG                                                                                                                         <  1:51249/149‑1
                                CTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGA                                                                                                                              <  8:34255/127‑1
                                CTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGA                                                                                                                              >  7:34255/1‑127
                                       CCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGA                                                                                                 <  3:16244/149‑1
                                             ACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATG                                                                                           <  5:143551/149‑1
                                                TCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTT                                                                                        >  7:10282/1‑149
                                                  AATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTTTAGATTCAGTGGGCCAGTCTACAGTGGTCGGGAGAACTCAGACATACGTGATAAGCCGCTGATAGATGTTTT                                                                                       <  6:289034/148‑1
                                                   ATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCG                                                                                     >  8:123630/1‑149
                                                          GTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGC                                                                              >  8:320661/1‑149
                                                             CTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCT                                                                            <  4:329556/148‑1
                                                                CAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTA                                                                        >  1:205681/1‑149
                                                                CAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTT                                                                         <  7:320661/148‑1
                                                                     ACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTG                                                                    <  7:123630/148‑1
                                                                      CTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAATTGATATTGGTTGTCTGGAGATTCAGGGTGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGTATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGAC                                                                  <  6:21895/149‑1
                                                                      CTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCATTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGAC                                                                  <  6:27378/149‑1
                                                                         GTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGT                                                                >  3:252482/1‑148
                                                                            TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATT                                                            >  3:253354/1‑149
                                                                            TAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTC                                                           >  4:195677/1‑149
                                                                                            caaTATCACTTAAATAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAG                                            >  6:236110/4‑149
                                                                                                       ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAGCATCTGATTGG                                 >  4:69222/1‑149
                                                                                                       ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAGCATCTGATTGG                                 >  3:205423/1‑149
                                                                                                          TAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCTCTGATAGATGTTTTCGGGTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAACCATCTGATTGGCA                               <  2:158234/148‑1
                                                                                                                   TTGGTTGTCTGGAGATTCAGGGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAGCATCTGATTGGCACTATGCCGGG                     <  2:64360/149‑1
                                                                                                                          TCTGGAGATTCAGGGGGCCAGTCTAGCGTGGTCGGGAGAAAGCTGAAATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCATTCAGAAAAAGCATCTGATTGGCACTATGCCGGGGCGGCGT              <  4:252482/149‑1
                                                                                                                                       GGGGCCAGTCTACCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAGCATCTGATTGGCACTATGCCGGGGCGGCGTCTGGCTTTGGTA  >  2:35959/1‑148
                                                                                                                                                                                                                                                                                           
ATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3186242‑3186096
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGTGGTCGGGAGAACGCAGACATACGGGATAAGCCGCTGATAGATGTTTTCGGTTATGCCTTTTATCTGACGTAATTCGCCAGTCAGAAAAAGCATCTGATTGGCACTATGCCGGGGCGGCGTCTGGCTTTGGTA  <  NC_000913/3463560‑3463425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.