New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 442074634 (0.950)6 (0.180) 6/234 3.4 16.7% coding (767/1398 nt) ulaA L‑ascorbate‑specific enzyme IIC permease component of PTS
?NC_000913 = 4420774 28 (0.830)coding (795/1398 nt) ulaA L‑ascorbate‑specific enzyme IIC permease component of PTS
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCGTTCCTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCATTTAACGGCATTTCCCAGCGCC  >  NC_000913/4420600‑4420892
                                                                                                                                                  |                                                                                                                                                  
gcCGTTCCTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGc                                                                                                                                                  <  5:163323/149‑1 (MQ=255)
  cGTTCCTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAg                                                                                                                                                <  6:114805/149‑1 (MQ=255)
      ccTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGa                                                                                                                                            >  6:119455/1‑149 (MQ=255)
            cAAAAAAGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATggcag                                                                                                                                      <  4:63866/149‑1 (MQ=255)
             aaaaaaGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATggcagg                                                                                                                                     <  3:105156/149‑1 (MQ=255)
               aaaaGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATggcaggc                                                                                                                                    >  1:148874/1‑148 (MQ=255)
                      gagaGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCa                                                                                                                            <  5:119455/149‑1 (MQ=255)
                                aaGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGa                                                                                                                                            >  3:55493/1‑123 (MQ=255)
                                aaGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGa                                                                                                                                            <  4:55493/123‑1 (MQ=255)
                                  gACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTa                                                                                                                <  4:158892/149‑1 (MQ=255)
                                                      gctgAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTtct                                                                                            >  2:24844/1‑149 (MQ=255)
                                                       ctgAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTtctc                                                                                           <  2:146288/149‑1 (MQ=255)
                                                             aTTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGc                                                                                     <  3:26111/149‑1 (MQ=255)
                                                               tttCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGTCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTtctc                                                                                           >  7:49976/1‑141 (MQ=255)
                                                               tttCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGTCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTtctc                                                                                           <  8:49976/141‑1 (MQ=255)
                                                                   cACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTgg                                                                                <  2:148874/148‑1 (MQ=255)
                                                                                                  tgaCCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGc                                                              <  3:101496/135‑1 (MQ=255)
                                                                                                  tgaCCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGc                                                              >  4:101496/1‑135 (MQ=255)
                                                                                                     ccATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGttt                                             <  7:99958/149‑1 (MQ=255)
                                                                                                     ccATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGttt                                             >  8:99958/1‑149 (MQ=255)
                                                                                                         cttcttTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGa                                                                             <  4:112241/113‑1 (MQ=255)
                                                                                                         cttcttTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGa                                                                             >  3:112241/1‑113 (MQ=255)
                                                                                                         cttcttTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTgg                                         >  5:37872/1‑149 (MQ=255)
                                                                                                                          tGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTgg                                         >  4:85702/1‑132 (MQ=255)
                                                                                                                          tGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTgg                                         <  3:85702/132‑1 (MQ=255)
                                                                                                                           gCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAg                                                            <  4:19227/112‑1 (MQ=255)
                                                                                                                           gCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAg                                                            >  3:19227/1‑112 (MQ=255)
                                                                                                                           gCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCAt                       >  8:129188/1‑149 (MQ=255)
                                                                                                                            ctctCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCAtt                      >  2:119352/1‑149 (MQ=255)
                                                                                                                             tctcCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAgg                                                           >  2:77906/1‑111 (MQ=255)
                                                                                                                             tctcCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAgg                                                           <  1:77906/111‑1 (MQ=255)
                                                                                                                                       tATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCATTTAACGGCAttt           <  7:129188/149‑1 (MQ=255)
                                                                                                                                         tCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCATTTAACGGCATTTcc         <  1:24844/149‑1 (MQ=255)
                                                                                                                                                cGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCATTTAACGGCATTTCCCAGCGcc  <  1:119352/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCGTTCCTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTCAAATTGCCGGGCTGGCTGAACATTTTCCACGACAACATCGTCTCCACGGCGATTGTGATGACCATCTTCTTTGGTGCCATTCTGCTCTCCTTCGGTATCGACACCGTGCAGGCGATGGCAGGCAAAGTGCACTGGACGGTGTACATCCTGCAAACTGGTTTCTCCTTTGCGGTGGCGATCTTCATCATCACGCAGGGTGTGCGCATGTTTGTGGCGGAACTCTCTGAAGCATTTAACGGCATTTCCCAGCGCC  >  NC_000913/4420600‑4420892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.