mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-1-BOP-27-RPE-END-4_S40_L001_R1_001.good.fq | 403,410 | 56,413,379 | 100.0% | 139.8 bases | 149 bases | 95.1% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L001_R2_001.good.fq | 403,410 | 55,822,819 | 100.0% | 138.4 bases | 149 bases | 97.1% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L002_R1_001.good.fq | 379,286 | 53,018,559 | 100.0% | 139.8 bases | 149 bases | 95.3% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L002_R2_001.good.fq | 379,286 | 52,506,720 | 100.0% | 138.4 bases | 149 bases | 96.9% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L003_R1_001.good.fq | 390,687 | 54,581,906 | 100.0% | 139.7 bases | 149 bases | 94.9% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L003_R2_001.good.fq | 390,687 | 54,008,026 | 100.0% | 138.2 bases | 149 bases | 97.0% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L004_R1_001.good.fq | 408,541 | 57,031,188 | 100.0% | 139.6 bases | 149 bases | 95.3% |
| errors | Plate-1-BOP-27-RPE-END-4_S40_L004_R2_001.good.fq | 408,541 | 56,488,301 | 100.0% | 138.3 bases | 149 bases | 97.2% |
| total | 3,163,848 | 439,870,898 | 100.0% | 139.0 bases | 149 bases | 96.1% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 80.1 | 3.7 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 31813 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 26 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.002 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.75456 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 10:33:09 06 Apr 2022 | 10:34:02 06 Apr 2022 | 53 seconds |
| Read alignment to reference genome | 10:34:03 06 Apr 2022 | 10:39:46 06 Apr 2022 | 5 minutes 43 seconds |
| Preprocessing alignments for candidate junction identification | 10:39:46 06 Apr 2022 | 10:40:23 06 Apr 2022 | 37 seconds |
| Preliminary analysis of coverage distribution | 10:40:23 06 Apr 2022 | 10:42:03 06 Apr 2022 | 1 minute 40 seconds |
| Identifying junction candidates | 10:42:03 06 Apr 2022 | 10:42:22 06 Apr 2022 | 19 seconds |
| Re-alignment to junction candidates | 10:42:22 06 Apr 2022 | 10:43:41 06 Apr 2022 | 1 minute 19 seconds |
| Resolving best read alignments | 10:43:41 06 Apr 2022 | 10:44:33 06 Apr 2022 | 52 seconds |
| Creating BAM files | 10:44:33 06 Apr 2022 | 10:46:04 06 Apr 2022 | 1 minute 31 seconds |
| Tabulating error counts | 10:46:04 06 Apr 2022 | 10:46:42 06 Apr 2022 | 38 seconds |
| Re-calibrating base error rates | 10:46:42 06 Apr 2022 | 10:46:44 06 Apr 2022 | 2 seconds |
| Examining read alignment evidence | 10:46:44 06 Apr 2022 | 10:54:08 06 Apr 2022 | 7 minutes 24 seconds |
| Polymorphism statistics | 10:54:08 06 Apr 2022 | 10:54:08 06 Apr 2022 | 0 seconds |
| Output | 10:54:08 06 Apr 2022 | 10:54:18 06 Apr 2022 | 10 seconds |
| Total | 21 minutes 8 seconds | ||
