mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | KT2440_Van_A3_F108_I0_R1_S66_L001_R1_001.good.fq | 276,869 | 35,275,754 | 100.0% | 127.4 bases | 135 bases | 94.2% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L001_R2_001.good.fq | 276,869 | 35,276,671 | 100.0% | 127.4 bases | 135 bases | 91.3% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L002_R1_001.good.fq | 275,482 | 35,173,644 | 100.0% | 127.7 bases | 135 bases | 95.5% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L002_R2_001.good.fq | 275,482 | 35,173,095 | 100.0% | 127.7 bases | 135 bases | 93.8% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L003_R1_001.good.fq | 277,103 | 35,410,011 | 100.0% | 127.8 bases | 135 bases | 94.1% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L003_R2_001.good.fq | 277,103 | 35,408,806 | 100.0% | 127.8 bases | 135 bases | 92.2% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L004_R1_001.good.fq | 278,159 | 35,684,106 | 100.0% | 128.3 bases | 136 bases | 95.5% |
| errors | KT2440_Van_A3_F108_I0_R1_S66_L004_R2_001.good.fq | 278,159 | 35,684,087 | 100.0% | 128.3 bases | 136 bases | 93.8% |
| total | 2,215,226 | 283,086,174 | 100.0% | 127.8 bases | 136 bases | 93.8% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | KT2440 | 6,185,276 | 42.6 | 2.9 | 100.0% | tig00000001|arrow. |
| total | 6,185,276 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 951 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 67 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.003 |
| reference sequence | pr(no read start) |
|---|---|
| KT2440 | 0.88128 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 22:00:46 01 May 2023 | 22:01:28 01 May 2023 | 42 seconds |
| Read alignment to reference genome | 22:01:28 01 May 2023 | 22:06:31 01 May 2023 | 5 minutes 3 seconds |
| Preprocessing alignments for candidate junction identification | 22:06:31 01 May 2023 | 22:07:00 01 May 2023 | 29 seconds |
| Preliminary analysis of coverage distribution | 22:07:00 01 May 2023 | 22:08:19 01 May 2023 | 1 minute 19 seconds |
| Identifying junction candidates | 22:08:19 01 May 2023 | 22:08:19 01 May 2023 | 0 seconds |
| Re-alignment to junction candidates | 22:08:19 01 May 2023 | 22:09:24 01 May 2023 | 1 minute 5 seconds |
| Resolving best read alignments | 22:09:24 01 May 2023 | 22:10:03 01 May 2023 | 39 seconds |
| Creating BAM files | 22:10:03 01 May 2023 | 22:11:15 01 May 2023 | 1 minute 12 seconds |
| Tabulating error counts | 22:11:15 01 May 2023 | 22:11:38 01 May 2023 | 23 seconds |
| Re-calibrating base error rates | 22:11:38 01 May 2023 | 22:11:41 01 May 2023 | 3 seconds |
| Examining read alignment evidence | 22:11:41 01 May 2023 | 22:28:39 01 May 2023 | 16 minutes 58 seconds |
| Polymorphism statistics | 22:28:39 01 May 2023 | 22:28:39 01 May 2023 | 0 seconds |
| Output | 22:28:39 01 May 2023 | 22:29:15 01 May 2023 | 36 seconds |
| Total | 28 minutes 29 seconds | ||
