breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 44,293 | Δ1 bp | 100% | intergenic (+124/‑367) | oprD_1 → / → czcR_2 | Porin D/Transcriptional activator protein CzcR |
RA | 96,480 | T→G | 32.1% | intergenic (+121/‑72) | cynT → / → CJ019_00104 | Carbonic anhydrase 1/hypothetical protein |
RA | 120,975 | Δ1 bp | 100% | intergenic (+272/+100) | polA → / ← engB | DNA polymerase I/putative GTP‑binding protein EngB |
RA | 179,484 | T→C | 100% | C316R (TGC→CGC) | CJ019_00182 → | hypothetical protein |
RA | 219,812 | C→T | 6.3% | L14L (CTG→TTG) | slyA_1 → | Transcriptional regulator SlyA |
RA | 253,344 | T→G | 19.0% | L137V (TTG→GTG) | ssuC_1 → | Putative aliphatic sulfonates transport permeaseprotein SsuC |
RA | 288,790 | C→G | 25.4% | intergenic (+90/+52) | CJ019_00262 → / ← CJ019_00263 | hypothetical protein/hypothetical protein |
RA | 345,224 | C→T | 6.4% | A220A (GCC→GCT) | hisA → | 1‑(5‑phosphoribosyl)‑5‑[(5‑phosphoribosylamino)m ethylideneamino] imidazole‑4‑carboxamide isomerase |
RA | 359,946 | T→A | 8.6% | E140V (GAG→GTG) | CJ019_00333 ← | hypothetical protein |
RA | 407,069 | A→G | 15.4% | intergenic (+39/‑164) | glnE → / → rfaF | Bifunctional glutamine synthetase adenylyltransferase/adenylyl‑removing enzyme/ADP‑heptose‑‑LPS heptosyltransferase 2 |
RA | 407,297 | A→T | 7.1% | Q22L (CAG→CTG) | rfaF → | ADP‑heptose‑‑LPS heptosyltransferase 2 |
RA | 450,509 | T→C | 8.9% | T108A (ACC→GCC) | pqqE ← | PqqA peptide cyclase |
RA | 710,940 | A→G | 8.7% | V186V (GTA→GTG) | thiO → | Glycine oxidase |
RA | 752,516 | A→C | 60.0% | intergenic (+438/‑396) | CJ019_00719 → / → CJ019_00721 | hypothetical protein/hypothetical protein |
RA | 778,930 | C→G | 23.6% | A16P (GCA→CCA) | CJ019_00742 ← | hypothetical protein |
RA | 933,782 | G→A | 8.7% | A1630A (GCC→GCT) | CJ019_00881 ← | hypothetical protein |
RA | 1,095,616 | T→A | 12.2% | S2R (AGT→AGA) | CJ019_01035 → | hypothetical protein |
RA | 1,378,944 | T→G | 29.3% | N189T (AAC→ACC) | oprD_6 ← | Porin D |
RA | 1,481,837 | T→C | 8.0% | L114P (CTG→CCG) | CJ019_01397 → | GTP cyclohydrolase 1 type 2 |
RA | 1,523,327 | T→A | 6.9% | intergenic (+97/‑16) | ftsZ → / → lpxC | Cell division protein FtsZ/UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase |
RA | 1,670,685 | A→C | 27.7% | intergenic (+22/‑109) | thrC_2 → / → CJ019_01569 | Threonine synthase/hypothetical protein |
RA | 1,692,266 | G→T | 100% | E320* (GAA→TAA) | cheB_1 → | Protein‑glutamatemethylesterase/protein‑glutamine glutaminase |
RA | 1,720,951 | T→G | 56.1% | D90A (GAC→GCC) | plsB ← | Glycerol‑3‑phosphate acyltransferase |
RA | 1,805,251 | C→T | 6.9% | D75N (GAT→AAT) | dmlR_4 ← | HTH‑type transcriptional regulator DmlR |
RA | 1,831,738 | T→G | 30.4% | S163R (AGC→CGC) | CJ019_01748 ← | NAD(P)H dehydrogenase (quinone) |
RA | 1,845,590 | C→T | 10.2% | A540A (GCC→GCT) | relA → | GTP pyrophosphokinase |
RA | 2,055,416 | A→T | 8.0% | N425I (AAT→ATT) | CJ019_01952 → | hypothetical protein |
RA | 2,094,808 | A→C | 51.0% | L186R (CTG→CGG) | CJ019_01995 ← | hypothetical protein |
RA | 2,094,817 | T→G | 61.2% | Q183P (CAG→CCG) | CJ019_01995 ← | hypothetical protein |
RA | 2,230,919 | A→T | 9.8% | V241D (GTC→GAC) | emrB_1 ← | Colistin resistance protein EmrB |
RA | 2,387,001 | A→C | 52.2% | intergenic (+70/+36) | CJ019_02252 → / ← dacC_1 | hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC |
RA | 2,417,619 | A→C | 24.7% | D44A (GAC→GCC) | yegS → | Lipid kinase YegS |
RA | 2,465,846 | G→A | 8.1% | A436V (GCG→GTG) | CJ019_02325 ← | hypothetical protein |
RA | 2,471,589 | G→A | 20.0% | L45L (CTG→CTA) | CJ019_02330 → | hypothetical protein |
RA | 2,595,295 | T→C | 9.7% | M1T (ATG→ACG) † | CJ019_02454 → | hypothetical protein |
RA | 2,759,723 | A→T | 7.7% | L665* (TTG→TAG) | cntO_2 ← | Metal‑pseudopaline receptor CntO |
RA | 3,061,793 | G→A | 100% | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
RA | 3,096,515 | G→A | 7.5% | L215L (CTG→CTA) | qseB_2 → | Transcriptional regulatory protein QseB |
RA | 3,412,615 | A→T | 7.6% | A179A (GCT→GCA) | lexA_2 ← | LexA repressor |
RA | 3,440,348 | T→G | 54.2% | A19A (GCT→GCG) | CJ019_03266 → | hypothetical protein |
RA | 3,535,852 | T→C | 8.9% | intergenic (‑73/+33) | galE ← / ← CJ019_03355 | UDP‑glucose 4‑epimerase/hypothetical protein |
RA | 3,535,865 | A→T | 8.2% | intergenic (‑86/+20) | galE ← / ← CJ019_03355 | UDP‑glucose 4‑epimerase/hypothetical protein |
RA | 3,786,897 | T→G | 51.1% | D245A (GAC→GCC) | thlA_2 ← | Acetyl‑CoA acetyltransferase |
RA | 3,957,251 | G→A | 100% | P133L (CCG→CTG) | dmlR_13 ← | HTH‑type transcriptional regulator DmlR |
RA | 4,092,717 | C→T | 8.1% | A316T (GCG→ACG) | CJ019_03854 ← | hypothetical protein |
RA | 4,135,607 | A→C | 49.9% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
RA | 4,135,612 | T→G | 39.7% | W34G (TGG→GGG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
RA | 4,165,443 | T→G | 13.0% | N109H (AAC→CAC) | ribN ← | Riboflavin transporter |
RA | 4,212,263 | C→T | 7.1% | V232I (GTT→ATT) | CJ019_03953 ← | hypothetical protein |
RA | 4,245,145 | A→C | 47.6% | L178R (CTG→CGG) | CJ019_03982 ← | Crotonyl‑CoA hydratase |
RA | 4,287,611 | T→G | 26.3% | D13A (GAC→GCC) | CJ019_04019 ← | hypothetical protein |
RA | 4,289,845 | T→G | 41.4% | intergenic (+119/‑106) | CJ019_04021 → / → gloA | hypothetical protein/Lactoylglutathione lyase |
RA | 4,293,337 | A→T | 6.4% | Q108L (CAG→CTG) | CJ019_04028 → | hypothetical protein |
RA | 4,388,450 | A→T | 14.9% | V56E (GTG→GAG) | CJ019_04138 ← | hypothetical protein |
RA | 4,400,445 | G→A | 9.7% | I348I (ATC→ATT) | CJ019_04157 ← | hypothetical protein |
RA | 4,514,652 | Δ1 bp | 10.8% | coding (731/1257 nt) | icd_1 ← | Isocitrate dehydrogenase [NADP] |
RA | 4,677,569 | A→C | 17.7% | K187N (AAA→AAC) | CJ019_04418 → | hypothetical protein |
RA | 4,703,371 | G→A | 8.7% | P138S (CCG→TCG) | sixA ← | Phosphohistidine phosphatase SixA |
RA | 4,749,286 | T→G | 32.7% | S294A (TCG→GCG) | macB → | Macrolide export ATP‑binding/permease proteinMacB |
RA | 4,802,244 | T→A | 14.8% | intergenic (‑706/‑25) | CJ019_04509 ← / → dsbD_2 | hypothetical protein/Thiol:disulfide interchange protein DsbD |
RA | 4,809,650 | T→C | 9.1% | Y66H (TAC→CAC) | CJ019_04517 → | hypothetical protein |
RA | 4,879,225 | A→T | 6.8% | intergenic (‑25/‑108) | yedI ← / → rutR_3 | Inner membrane protein YedI/HTH‑type transcriptional regulator RutR |
RA | 5,022,441 | G→A | 14.3% | A295T (GCC→ACC) | doeA → | Ectoine hydrolase |
RA | 5,528,483 | A→T | 7.3% | intergenic (‑183/‑330) | braC_2 ← / → pncB2 | Leucine‑, isoleucine‑, valine‑, threonine‑, and alanine‑binding protein/Nicotinate phosphoribosyltransferase 2 |
RA | 5,539,546 | C→T | 8.4% | intergenic (‑412/+17) | CJ019_03622 ← / ← rlmB | IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB |
RA | 5,582,884 | G→A | 12.5% | T31T (ACC→ACT) | parE ← | DNA topoisomerase 4 subunit B |
RA | 5,757,234 | Δ1 bp | 100% | coding (776/834 nt) | CJ019_05400 → | hypothetical protein |
RA | 5,791,128 | G→A | 19.8% | P203S (CCA→TCA) | gltB ← | Glutamate synthase [NADPH] large chain |
RA | 5,856,018 | C→A | 7.7% | M202I (ATG→ATT) | cysA ← | Sulfate/thiosulfate import ATP‑binding proteinCysA |
RA | 5,865,465 | C→T | 8.8% | M341I (ATG→ATA) | ptsP ← | Phosphoenolpyruvate‑dependent phosphotransferasesystem |
RA | 5,908,523 | G→A | 6.4% | A291V (GCG→GTG) | argA ← | Amino‑acid acetyltransferase |
RA | 5,954,912 | G→A | 9.7% | E67E (GAG→GAA) | CJ019_05576 → | hypothetical protein |
RA | 5,960,590 | G→T | 9.5% | P313Q (CCG→CAG) | amtB_2 ← | Ammonia channel |
RA | 6,014,104 | C→T | 5.9% | G127D (GGC→GAC) | dadA1_4 ← | D‑amino acid dehydrogenase 1 |
RA | 6,021,216 | A→G | 8.2% | F159S (TTC→TCC) | ycaD_4 ← | putative MFS‑type transporter YcaD |
RA | 6,167,267 | T→C | 12.8% | N403D (AAC→GAC) | glmU ← | Bifunctional protein GlmU |
RA | 6,174,824 | T→G | 31.5% | N153T (AAC→ACC) | atpB ← | ATP synthase subunit a |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | KT2440 | 88013 | 88060 | 48 | 10 [8] | [9] 14 | [CJ019_00095] | [CJ019_00095] |
* | * | ÷ | KT2440 | 161234 | 161387–161315 | 82–154 | 11 [9] | [9] 10 | [uctC_1] | [uctC_1] |
* | * | ÷ | KT2440 | 277604 | 277685 | 82 | 10 [8] | [9] 10 | [tauD_2] | [tauD_2] |
* | * | ÷ | KT2440 | 346098 | 346240 | 143 | 12 [7] | [9] 10 | [btuD_1] | [btuD_1] |
* | * | ÷ | KT2440 | 517190 | 517274 | 85 | 22 [9] | [9] 10 | tyrS/birA | Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA |
* | * | ÷ | KT2440 | 607304 | 607400 | 97 | 10 [9] | [7] 11 | dxs/ispA | 1‑deoxy‑D‑xylulose‑5‑phosphate synthase/Farnesyl diphosphate synthase |
* | * | ÷ | KT2440 | 641266 | 641335 | 70 | 10 [8] | [9] 10 | [acoR_2] | [acoR_2] |
* | * | ÷ | KT2440 | 661103 | 661232 | 130 | 10 [8] | [9] 10 | yfhM/CJ019_00619 | Alpha‑2‑macroglobulin/hypothetical protein |
* | * | ÷ | KT2440 | 674246 | 674380 | 135 | 10 [9] | [8] 13 | [nimR_1]–[mcpH_2] | [nimR_1],[mcpH_2] |
* | * | ÷ | KT2440 | 711482 | 711536 | 55 | 10 [6] | [9] 10 | thiO/aam | Glycine oxidase/Acylamidase |
* | * | ÷ | KT2440 | 856908 | 856981 | 74 | 11 [9] | [8] 16 | [hemH]–[uraA] | [hemH],[uraA] |
* | * | ÷ | KT2440 | 908071 | 908237 | 167 | 11 [8] | [9] 10 | [fruA]–[CJ019_00870] | [fruA],[CJ019_00870] |
* | * | ÷ | KT2440 | 1360628 | 1360980 | 353 | 10 [9] | [9] 11 | CJ019_01275 | hypothetical protein |
* | * | ÷ | KT2440 | 1376293 | 1376337 | 45 | 14 [5] | [9] 10 | CJ019_01289 | hypothetical protein |
* | * | ÷ | KT2440 | 1396599 | 1396688 | 90 | 10 [8] | [9] 10 | [mcpU_1] | [mcpU_1] |
* | * | ÷ | KT2440 | 1457213 | 1457294 | 82 | 11 [8] | [9] 10 | [algL]–[algX] | [algL],[algX] |
* | * | ÷ | KT2440 | 1492461 | 1492596 | 136 | 12 [9] | [8] 11 | [CJ019_01406] | [CJ019_01406] |
* | * | ÷ | KT2440 | 1499923 | 1500061 | 139 | 10 [9] | [8] 11 | [petC]–[sspA] | [petC],[sspA] |
* | * | ÷ | KT2440 | 1608676 | 1608791 | 116 | 10 [9] | [8] 10 | CJ019_01513 | hypothetical protein |
* | * | ÷ | KT2440 | 1632609 | 1632674 | 66 | 10 [9] | [6] 10 | czcR_4/CJ019_01536 | Transcriptional activator protein CzcR/hypothetical protein |
* | * | ÷ | KT2440 | 1697642 | 1697754 | 113 | 10 [7] | [9] 10 | [CJ019_01593]–[CJ019_01594] | [CJ019_01593],[CJ019_01594] |
* | * | ÷ | KT2440 | 1705089 | 1705195 | 107 | 12 [9] | [9] 10 | ppc/adk | Phosphoenolpyruvate carboxylase/Adenylate kinase |
* | * | ÷ | KT2440 | 1835836 | 1835886 | 51 | 11 [9] | [9] 10 | barA | Signal transduction histidine‑protein kinaseBarA |
* | * | ÷ | KT2440 | 1914560 | 1914687 | 128 | 15 [8] | [9] 10 | [ppsC_1]–[CJ019_01830] | [ppsC_1],[CJ019_01830] |
* | * | ÷ | KT2440 | 2234254 | 2234415 | 162 | 10 [9] | [7] 10 | [gltX] | [gltX] |
* | * | ÷ | KT2440 | 2288805 | 2288934 | 130 | 14 [9] | [8] 14 | [CJ019_02171] | [CJ019_02171] |
* | * | ÷ | KT2440 | 2393567 | 2393615 | 49 | 10 [9] | [8] 10 | [dgt] | [dgt] |
* | * | ÷ | KT2440 | 2786787 | 2786833 | 47 | 10 [9] | [7] 10 | CJ019_02632/CJ019_02633 | hypothetical protein/hypothetical protein |
* | * | ÷ | KT2440 | 2830089 | 2830238 | 150 | 10 [8] | [6] 11 | [gabD1] | [gabD1] |
* | * | ÷ | KT2440 | 2942843 | 2942872 | 30 | 10 [7] | [6] 11 | CJ019_02780/CJ019_02781 | hypothetical protein/8‑oxoguanine deaminase |
* | * | ÷ | KT2440 | 2944350 | 2944441 | 92 | 15 [5] | [9] 11 | [CJ019_02782] | [CJ019_02782] |
* | * | ÷ | KT2440 | 3046193 | 3046353 | 161 | 10 [9] | [9] 10 | [rcsC_5]–[degU_2] | [rcsC_5],[degU_2] |
* | * | ÷ | KT2440 | 3145305 | 3145396 | 92 | 10 [8] | [9] 12 | livH_4 | High‑affinity branched‑chain amino acidtransport system permease protein LivH |
* | * | ÷ | KT2440 | 3459859 | 3459971 | 113 | 11 [9] | [9] 10 | [CJ019_03284] | [CJ019_03284] |
* | * | ÷ | KT2440 | 3471024 | 3471085 | 62 | 15 [8] | [9] 10 | CJ019_03292/hcp1_1 | hypothetical protein/Protein hcp1 |
* | * | ÷ | KT2440 | 3769404 | 3769507 | 104 | 12 [9] | [9] 10 | CJ019_03581/CJ019_03582 | hypothetical protein/hypothetical protein |
* | * | ÷ | KT2440 | 4077416 | 4077464 | 49 | 10 [8] | [8] 10 | artJ_2/hisQ_1 | ABC transporter arginine‑binding protein 1/Histidine transport system permease proteinHisQ |
* | * | ÷ | KT2440 | 4163797 | 4163863 | 67 | 11 [9] | [9] 12 | katG/leuO_2 | Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO |
* | * | ÷ | KT2440 | 4346803 | 4346849 | 47 | 11 [8] | [9] 11 | nmoA | Nitronate monooxygenase |
* | * | ÷ | KT2440 | 4523612 | 4523708 | 97 | 10 [7] | [9] 11 | [CJ019_04289] | [CJ019_04289] |
* | * | ÷ | KT2440 | 4668931 | 4669025 | 95 | 10 [9] | [9] 10 | [btuB_4] | [btuB_4] |
* | * | ÷ | KT2440 | 4734443 | 4734518 | 76 | 10 [8] | [8] 10 | gltA/CJ019_04468 | Citrate synthase/hypothetical protein |
* | * | ÷ | KT2440 | 4790221 | 4790309 | 89 | 11 [7] | [9] 11 | [qseC_3] | [qseC_3] |
* | * | ÷ | KT2440 | 4801762 | 4802163 | 402 | 10 [9] | [9] 10 | CJ019_04509/dsbD_2 | hypothetical protein/Thiol:disulfide interchange protein DsbD |
* | * | ÷ | KT2440 | 4838975 | 4839076 | 102 | 12 [8] | [7] 13 | [copA_3] | [copA_3] |
* | * | ÷ | KT2440 | 4846236 | 4846350 | 115 | 10 [9] | [9] 10 | recR/ybaB | Recombination protein RecR/Nucleoid‑associated protein YbaB |
* | * | ÷ | KT2440 | 4883369 | 4883485 | 117 | 10 [9] | [9] 17 | [hyi] | [hyi] |
* | * | ÷ | KT2440 | 4944746 | 4944831 | 86 | 10 [9] | [7] 10 | [CJ019_04642] | [CJ019_04642] |
* | * | ÷ | KT2440 | 5156299 | 5156448 | 150 | 11 [9] | [9] 14 | [CJ019_04852] | [CJ019_04852] |
* | * | ÷ | KT2440 | 5188696 | 5188747 | 52 | 10 [9] | [8] 10 | [pxpC_2] | [pxpC_2] |
* | * | ÷ | KT2440 | 5193445 | 5193528 | 84 | 12 [9] | [9] 12 | [gltR_4]–[CJ019_04888] | [gltR_4],[CJ019_04888] |
* | * | ÷ | KT2440 | 5250635 | 5250729 | 95 | 10 [8] | [8] 12 | [CJ019_04947]–[CJ019_04948] | [CJ019_04947],[CJ019_04948] |
* | * | ÷ | KT2440 | 5478944 | 5479048 | 105 | 10 [9] | [9] 10 | purH/purD | Bifunctional purine biosynthesis protein PurH/Phosphoribosylamine‑‑glycine ligase |
* | * | ÷ | KT2440 | 5500300 | 5500442 | 143 | 12 [7] | [9] 10 | [CJ019_05176] | [CJ019_05176] |
* | * | ÷ | KT2440 | 5530814 | 5530936 | 123 | 11 [9] | [9] 10 | [nadE] | [nadE] |
* | * | ÷ | KT2440 | 5551638 | 5551812 | 175 | 11 [9] | [9] 11 | [purA] | [purA] |
* | * | ÷ | KT2440 | 5646789 | 5646845 | 57 | 10 [9] | [9] 10 | CJ019_05302/CJ019_05303 | hypothetical protein/hypothetical protein |
* | * | ÷ | KT2440 | 5734267 | 5734329 | 63 | 11 [7] | [8] 10 | [CJ019_05378] | [CJ019_05378] |
* | * | ÷ | KT2440 | 5896948 | 5897040 | 93 | 10 [9] | [9] 13 | [CJ019_05521] | [CJ019_05521] |
* | * | ÷ | KT2440 | 5914028 | 5914116 | 89 | 10 [9] | [9] 10 | CJ019_05537 | hypothetical protein |
* | * | ÷ | KT2440 | 5937442 | 5937528 | 87 | 10 [9] | [8] 10 | [chaC] | [chaC] |
* | * | ÷ | KT2440 | 6074778 | 6074886 | 109 | 10 [7] | [8] 10 | [yeaD_2]–[CJ019_05690] | [yeaD_2],[CJ019_05690] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | KT2440 | = 3542695 | 19 (0.490) | 20 (0.530) | 17/224 | NT | 57.8% | coding (970/1467 nt) | betA_1 | Oxygen‑dependent choline dehydrogenase |
? | KT2440 | 3543005 = | 11 (0.290) | coding (1280/1467 nt) | betA_1 | Oxygen‑dependent choline dehydrogenase | |||||
* | ? | KT2440 | 4918800 = | 0 (0.000) | 51 (1.340) | 28/228 | NT | 100% | coding (7/789 nt) | soj_1 | Sporulation initiation inhibitor protein Soj |
? | KT2440 | = 5539492 | NA (NA) | intergenic (‑358/+71) | CJ019_03622/rlmB | IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB | |||||
* | ? | KT2440 | = 4918801 | 0 (0.000) | 35 (0.910) | 25/230 | NT | 100% | coding (6/789 nt) | soj_1 | Sporulation initiation inhibitor protein Soj |
? | KT2440 | 5216408 = | NA (NA) | intergenic (‑50/+386) | rhaS_5/CJ019_01212 | HTH‑type transcriptional activator RhaS/IS110 family transposase ISPpu9 |