breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 96,480 T→G 32.1% intergenic (+121/‑72) cynT → / → CJ019_00104 Carbonic anhydrase 1/hypothetical protein
RA 120,975 Δ1 bp 100% intergenic (+272/+100) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 179,484 T→C 100% C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 219,812 C→T 6.3% L14L (CTG→TTG)  slyA_1 → Transcriptional regulator SlyA
RA 253,344 T→G 19.0% L137V (TTG→GTG)  ssuC_1 → Putative aliphatic sulfonates transport permeaseprotein SsuC
RA 288,790 C→G 25.4% intergenic (+90/+52) CJ019_00262 → / ← CJ019_00263 hypothetical protein/hypothetical protein
RA 345,224 C→T 6.4% A220A (GCC→GCT hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑phosphoribosylamino)m ethylideneamino] imidazole‑4‑carboxamide isomerase
RA 359,946 T→A 8.6% E140V (GAG→GTG)  CJ019_00333 ← hypothetical protein
RA 407,069 A→G 15.4% intergenic (+39/‑164) glnE → / → rfaF Bifunctional glutamine synthetase adenylyltransferase/adenylyl‑removing enzyme/ADP‑heptose‑‑LPS heptosyltransferase 2
RA 407,297 A→T 7.1% Q22L (CAG→CTG)  rfaF → ADP‑heptose‑‑LPS heptosyltransferase 2
RA 450,509 T→C 8.9% T108A (ACC→GCC)  pqqE ← PqqA peptide cyclase
RA 710,940 A→G 8.7% V186V (GTA→GTG thiO → Glycine oxidase
RA 752,516 A→C 60.0% intergenic (+438/‑396) CJ019_00719 → / → CJ019_00721 hypothetical protein/hypothetical protein
RA 778,930 C→G 23.6% A16P (GCA→CCA)  CJ019_00742 ← hypothetical protein
RA 933,782 G→A 8.7% A1630A (GCC→GCT CJ019_00881 ← hypothetical protein
RA 1,095,616 T→A 12.2% S2R (AGT→AGA CJ019_01035 → hypothetical protein
RA 1,378,944 T→G 29.3% N189T (AAC→ACC)  oprD_6 ← Porin D
RA 1,481,837 T→C 8.0% L114P (CTG→CCG)  CJ019_01397 → GTP cyclohydrolase 1 type 2
RA 1,523,327 T→A 6.9% intergenic (+97/‑16) ftsZ → / → lpxC Cell division protein FtsZ/UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
RA 1,670,685 A→C 27.7% intergenic (+22/‑109) thrC_2 → / → CJ019_01569 Threonine synthase/hypothetical protein
RA 1,692,266 G→T 100% E320* (GAA→TAA)  cheB_1 → Protein‑glutamatemethylesterase/protein‑glutamine glutaminase
RA 1,720,951 T→G 56.1% D90A (GAC→GCC)  plsB ← Glycerol‑3‑phosphate acyltransferase
RA 1,805,251 C→T 6.9% D75N (GAT→AAT)  dmlR_4 ← HTH‑type transcriptional regulator DmlR
RA 1,831,738 T→G 30.4% S163R (AGC→CGC)  CJ019_01748 ← NAD(P)H dehydrogenase (quinone)
RA 1,845,590 C→T 10.2% A540A (GCC→GCT relA → GTP pyrophosphokinase
RA 2,055,416 A→T 8.0% N425I (AAT→ATT)  CJ019_01952 → hypothetical protein
RA 2,094,808 A→C 51.0% L186R (CTG→CGG)  CJ019_01995 ← hypothetical protein
RA 2,094,817 T→G 61.2% Q183P (CAG→CCG)  CJ019_01995 ← hypothetical protein
RA 2,230,919 A→T 9.8% V241D (GTC→GAC)  emrB_1 ← Colistin resistance protein EmrB
RA 2,387,001 A→C 52.2% intergenic (+70/+36) CJ019_02252 → / ← dacC_1 hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 2,417,619 A→C 24.7% D44A (GAC→GCC)  yegS → Lipid kinase YegS
RA 2,465,846 G→A 8.1% A436V (GCG→GTG)  CJ019_02325 ← hypothetical protein
RA 2,471,589 G→A 20.0% L45L (CTG→CTA CJ019_02330 → hypothetical protein
RA 2,595,295 T→C 9.7% M1T (ATG→ACG) † CJ019_02454 → hypothetical protein
RA 2,759,723 A→T 7.7% L665* (TTG→TAG)  cntO_2 ← Metal‑pseudopaline receptor CntO
RA 3,061,793 G→A 100% S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)
RA 3,096,515 G→A 7.5% L215L (CTG→CTA qseB_2 → Transcriptional regulatory protein QseB
RA 3,412,615 A→T 7.6% A179A (GCT→GCA lexA_2 ← LexA repressor
RA 3,440,348 T→G 54.2% A19A (GCT→GCG CJ019_03266 → hypothetical protein
RA 3,535,852 T→C 8.9% intergenic (‑73/+33) galE ← / ← CJ019_03355 UDP‑glucose 4‑epimerase/hypothetical protein
RA 3,535,865 A→T 8.2% intergenic (‑86/+20) galE ← / ← CJ019_03355 UDP‑glucose 4‑epimerase/hypothetical protein
RA 3,786,897 T→G 51.1% D245A (GAC→GCC)  thlA_2 ← Acetyl‑CoA acetyltransferase
RA 3,957,251 G→A 100% P133L (CCG→CTG)  dmlR_13 ← HTH‑type transcriptional regulator DmlR
RA 4,092,717 C→T 8.1% A316T (GCG→ACG)  CJ019_03854 ← hypothetical protein
RA 4,135,607 A→C 49.9% Q32P (CAG→CCG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,135,612 T→G 39.7% W34G (TGG→GGG)  tgnC → (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
RA 4,165,443 T→G 13.0% N109H (AAC→CAC)  ribN ← Riboflavin transporter
RA 4,212,263 C→T 7.1% V232I (GTT→ATT)  CJ019_03953 ← hypothetical protein
RA 4,245,145 A→C 47.6% L178R (CTG→CGG)  CJ019_03982 ← Crotonyl‑CoA hydratase
RA 4,287,611 T→G 26.3% D13A (GAC→GCC)  CJ019_04019 ← hypothetical protein
RA 4,289,845 T→G 41.4% intergenic (+119/‑106) CJ019_04021 → / → gloA hypothetical protein/Lactoylglutathione lyase
RA 4,293,337 A→T 6.4% Q108L (CAG→CTG)  CJ019_04028 → hypothetical protein
RA 4,388,450 A→T 14.9% V56E (GTG→GAG)  CJ019_04138 ← hypothetical protein
RA 4,400,445 G→A 9.7% I348I (ATC→ATT CJ019_04157 ← hypothetical protein
RA 4,514,652 Δ1 bp 10.8% coding (731/1257 nt) icd_1 ← Isocitrate dehydrogenase [NADP]
RA 4,677,569 A→C 17.7% K187N (AAA→AAC CJ019_04418 → hypothetical protein
RA 4,703,371 G→A 8.7% P138S (CCG→TCG)  sixA ← Phosphohistidine phosphatase SixA
RA 4,749,286 T→G 32.7% S294A (TCG→GCG)  macB → Macrolide export ATP‑binding/permease proteinMacB
RA 4,802,244 T→A 14.8% intergenic (‑706/‑25) CJ019_04509 ← / → dsbD_2 hypothetical protein/Thiol:disulfide interchange protein DsbD
RA 4,809,650 T→C 9.1% Y66H (TAC→CAC)  CJ019_04517 → hypothetical protein
RA 4,879,225 A→T 6.8% intergenic (‑25/‑108) yedI ← / → rutR_3 Inner membrane protein YedI/HTH‑type transcriptional regulator RutR
RA 5,022,441 G→A 14.3% A295T (GCC→ACC)  doeA → Ectoine hydrolase
RA 5,528,483 A→T 7.3% intergenic (‑183/‑330) braC_2 ← / → pncB2 Leucine‑, isoleucine‑, valine‑, threonine‑, and alanine‑binding protein/Nicotinate phosphoribosyltransferase 2
RA 5,539,546 C→T 8.4% intergenic (‑412/+17) CJ019_03622 ← / ← rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
RA 5,582,884 G→A 12.5% T31T (ACC→ACT parE ← DNA topoisomerase 4 subunit B
RA 5,757,234 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein
RA 5,791,128 G→A 19.8% P203S (CCA→TCA)  gltB ← Glutamate synthase [NADPH] large chain
RA 5,856,018 C→A 7.7% M202I (ATG→ATT cysA ← Sulfate/thiosulfate import ATP‑binding proteinCysA
RA 5,865,465 C→T 8.8% M341I (ATG→ATA ptsP ← Phosphoenolpyruvate‑dependent phosphotransferasesystem
RA 5,908,523 G→A 6.4% A291V (GCG→GTG)  argA ← Amino‑acid acetyltransferase
RA 5,954,912 G→A 9.7% E67E (GAG→GAA CJ019_05576 → hypothetical protein
RA 5,960,590 G→T 9.5% P313Q (CCG→CAG)  amtB_2 ← Ammonia channel
RA 6,014,104 C→T 5.9% G127D (GGC→GAC)  dadA1_4 ← D‑amino acid dehydrogenase 1
RA 6,021,216 A→G 8.2% F159S (TTC→TCC)  ycaD_4 ← putative MFS‑type transporter YcaD
RA 6,167,267 T→C 12.8% N403D (AAC→GAC)  glmU ← Bifunctional protein GlmU
RA 6,174,824 T→G 31.5% N153T (AAC→ACC)  atpB ← ATP synthase subunit a

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ KT2440 88013 88060 48 10 [8] [9] 14 [CJ019_00095] [CJ019_00095]
* * ÷ KT2440 161234 161387–161315 82–154 11 [9] [9] 10 [uctC_1] [uctC_1]
* * ÷ KT2440 277604 277685 82 10 [8] [9] 10 [tauD_2] [tauD_2]
* * ÷ KT2440 346098 346240 143 12 [7] [9] 10 [btuD_1] [btuD_1]
* * ÷ KT2440 517190 517274 85 22 [9] [9] 10 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ KT2440 607304 607400 97 10 [9] [7] 11 dxs/ispA 1‑deoxy‑D‑xylulose‑5‑phosphate synthase/Farnesyl diphosphate synthase
* * ÷ KT2440 641266 641335 70 10 [8] [9] 10 [acoR_2] [acoR_2]
* * ÷ KT2440 661103 661232 130 10 [8] [9] 10 yfhM/CJ019_00619 Alpha‑2‑macroglobulin/hypothetical protein
* * ÷ KT2440 674246 674380 135 10 [9] [8] 13 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ KT2440 711482 711536 55 10 [6] [9] 10 thiO/aam Glycine oxidase/Acylamidase
* * ÷ KT2440 856908 856981 74 11 [9] [8] 16 [hemH]–[uraA] [hemH],[uraA]
* * ÷ KT2440 908071 908237 167 11 [8] [9] 10 [fruA]–[CJ019_00870] [fruA],[CJ019_00870]
* * ÷ KT2440 1360628 1360980 353 10 [9] [9] 11 CJ019_01275 hypothetical protein
* * ÷ KT2440 1376293 1376337 45 14 [5] [9] 10 CJ019_01289 hypothetical protein
* * ÷ KT2440 1396599 1396688 90 10 [8] [9] 10 [mcpU_1] [mcpU_1]
* * ÷ KT2440 1457213 1457294 82 11 [8] [9] 10 [algL]–[algX] [algL],[algX]
* * ÷ KT2440 1492461 1492596 136 12 [9] [8] 11 [CJ019_01406] [CJ019_01406]
* * ÷ KT2440 1499923 1500061 139 10 [9] [8] 11 [petC]–[sspA] [petC],[sspA]
* * ÷ KT2440 1608676 1608791 116 10 [9] [8] 10 CJ019_01513 hypothetical protein
* * ÷ KT2440 1632609 1632674 66 10 [9] [6] 10 czcR_4/CJ019_01536 Transcriptional activator protein CzcR/hypothetical protein
* * ÷ KT2440 1697642 1697754 113 10 [7] [9] 10 [CJ019_01593]–[CJ019_01594] [CJ019_01593],[CJ019_01594]
* * ÷ KT2440 1705089 1705195 107 12 [9] [9] 10 ppc/adk Phosphoenolpyruvate carboxylase/Adenylate kinase
* * ÷ KT2440 1835836 1835886 51 11 [9] [9] 10 barA Signal transduction histidine‑protein kinaseBarA
* * ÷ KT2440 1914560 1914687 128 15 [8] [9] 10 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ KT2440 2234254 2234415 162 10 [9] [7] 10 [gltX] [gltX]
* * ÷ KT2440 2288805 2288934 130 14 [9] [8] 14 [CJ019_02171] [CJ019_02171]
* * ÷ KT2440 2393567 2393615 49 10 [9] [8] 10 [dgt] [dgt]
* * ÷ KT2440 2786787 2786833 47 10 [9] [7] 10 CJ019_02632/CJ019_02633 hypothetical protein/hypothetical protein
* * ÷ KT2440 2830089 2830238 150 10 [8] [6] 11 [gabD1] [gabD1]
* * ÷ KT2440 2942843 2942872 30 10 [7] [6] 11 CJ019_02780/CJ019_02781 hypothetical protein/8‑oxoguanine deaminase
* * ÷ KT2440 2944350 2944441 92 15 [5] [9] 11 [CJ019_02782] [CJ019_02782]
* * ÷ KT2440 3046193 3046353 161 10 [9] [9] 10 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ KT2440 3145305 3145396 92 10 [8] [9] 12 livH_4 High‑affinity branched‑chain amino acidtransport system permease protein LivH
* * ÷ KT2440 3459859 3459971 113 11 [9] [9] 10 [CJ019_03284] [CJ019_03284]
* * ÷ KT2440 3471024 3471085 62 15 [8] [9] 10 CJ019_03292/hcp1_1 hypothetical protein/Protein hcp1
* * ÷ KT2440 3769404 3769507 104 12 [9] [9] 10 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ KT2440 4077416 4077464 49 10 [8] [8] 10 artJ_2/hisQ_1 ABC transporter arginine‑binding protein 1/Histidine transport system permease proteinHisQ
* * ÷ KT2440 4163797 4163863 67 11 [9] [9] 12 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ KT2440 4346803 4346849 47 11 [8] [9] 11 nmoA Nitronate monooxygenase
* * ÷ KT2440 4523612 4523708 97 10 [7] [9] 11 [CJ019_04289] [CJ019_04289]
* * ÷ KT2440 4668931 4669025 95 10 [9] [9] 10 [btuB_4] [btuB_4]
* * ÷ KT2440 4734443 4734518 76 10 [8] [8] 10 gltA/CJ019_04468 Citrate synthase/hypothetical protein
* * ÷ KT2440 4790221 4790309 89 11 [7] [9] 11 [qseC_3] [qseC_3]
* * ÷ KT2440 4801762 4802163 402 10 [9] [9] 10 CJ019_04509/dsbD_2 hypothetical protein/Thiol:disulfide interchange protein DsbD
* * ÷ KT2440 4838975 4839076 102 12 [8] [7] 13 [copA_3] [copA_3]
* * ÷ KT2440 4846236 4846350 115 10 [9] [9] 10 recR/ybaB Recombination protein RecR/Nucleoid‑associated protein YbaB
* * ÷ KT2440 4883369 4883485 117 10 [9] [9] 17 [hyi] [hyi]
* * ÷ KT2440 4944746 4944831 86 10 [9] [7] 10 [CJ019_04642] [CJ019_04642]
* * ÷ KT2440 5156299 5156448 150 11 [9] [9] 14 [CJ019_04852] [CJ019_04852]
* * ÷ KT2440 5188696 5188747 52 10 [9] [8] 10 [pxpC_2] [pxpC_2]
* * ÷ KT2440 5193445 5193528 84 12 [9] [9] 12 [gltR_4]–[CJ019_04888] [gltR_4],[CJ019_04888]
* * ÷ KT2440 5250635 5250729 95 10 [8] [8] 12 [CJ019_04947]–[CJ019_04948] [CJ019_04947],[CJ019_04948]
* * ÷ KT2440 5478944 5479048 105 10 [9] [9] 10 purH/purD Bifunctional purine biosynthesis protein PurH/Phosphoribosylamine‑‑glycine ligase
* * ÷ KT2440 5500300 5500442 143 12 [7] [9] 10 [CJ019_05176] [CJ019_05176]
* * ÷ KT2440 5530814 5530936 123 11 [9] [9] 10 [nadE] [nadE]
* * ÷ KT2440 5551638 5551812 175 11 [9] [9] 11 [purA] [purA]
* * ÷ KT2440 5646789 5646845 57 10 [9] [9] 10 CJ019_05302/CJ019_05303 hypothetical protein/hypothetical protein
* * ÷ KT2440 5734267 5734329 63 11 [7] [8] 10 [CJ019_05378] [CJ019_05378]
* * ÷ KT2440 5896948 5897040 93 10 [9] [9] 13 [CJ019_05521] [CJ019_05521]
* * ÷ KT2440 5914028 5914116 89 10 [9] [9] 10 CJ019_05537 hypothetical protein
* * ÷ KT2440 5937442 5937528 87 10 [9] [8] 10 [chaC] [chaC]
* * ÷ KT2440 6074778 6074886 109 10 [7] [8] 10 [yeaD_2]–[CJ019_05690] [yeaD_2],[CJ019_05690]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? KT2440 = 354269519 (0.490)20 (0.530) 17/224 NT 57.8% coding (970/1467 nt) betA_1 Oxygen‑dependent choline dehydrogenase
?KT2440 3543005 = 11 (0.290)coding (1280/1467 nt) betA_1 Oxygen‑dependent choline dehydrogenase
* ? KT2440 4918800 =0 (0.000)51 (1.340) 28/228 NT 100% coding (7/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 = 5539492 NA (NA)intergenic (‑358/+71) CJ019_03622/rlmB IS110 family transposase ISPpu9/23S rRNA (guanosine‑2'‑O‑)‑methyltransferaseRlmB
* ? KT2440 = 49188010 (0.000)35 (0.910) 25/230 NT 100% coding (6/789 nt) soj_1 Sporulation initiation inhibitor protein Soj
?KT2440 5216408 = NA (NA)intergenic (‑50/+386) rhaS_5/CJ019_01212 HTH‑type transcriptional activator RhaS/IS110 family transposase ISPpu9