New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 2902695 = | 54 (1.000) | 4 (0.080) | 4/280 | 7.4 | 6.8% | coding (331/1638 nt) | pyrG | CTP synthetase |
? | CP009273 | 2902717 = | 57 (1.100) | coding (309/1638 nt) | pyrG | CTP synthetase | |||||
Rejected: Coverage evenness skew score above cutoff. | |||||||||||
Rejected: Frequency below/above cutoff threshold. |
CAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGT > CP009273/2902573‑2902858 | cAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGAGATGTGCGGAATAACCTGCACGGTTGCGCGGAGGTAGTCACCGCGGCGTTCtt < 1:503959/149‑1 (MQ=255) tCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGACACCTCGGCGTTCTTTACGCAGAACGTc < 1:599616/149‑1 (MQ=255) ccTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCaga < 1:758377/149‑1 (MQ=255) tACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGGTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGCGGTAGTCACCGCGGCGTTCTTTACGCAGAACGt > 2:107062/1‑143 (MQ=255) ccgccgATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGGTCTTTGATTGCGTTAGTGATTTGCGGAATAACCTGCACGGTTGCGCAGAGGTAGTCACCGCGGCGTTCTTTAAGCCGAACGtacgagaa > 2:229126/1‑136 (MQ=255) cgccgATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAGGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCGCCGCGGCGTTCTTTACGCCGAActtgagggcag > 2:62694/1‑133 (MQ=255) cgccgATTTCTACCACTACTACGGCAGGGCCTGCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGa < 1:618071/149‑1 (MQ=255) gccgATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAAAGTCAGAGgaga > 2:351064/1‑143 (MQ=255) ttCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCgtgg < 1:606277/149‑1 (MQ=255) cGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTgcgg > 1:705554/1‑149 (MQ=255) aTGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCggcggc > 1:806545/1‑149 (MQ=255) aCCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGGTGcggcgggcca > 1:326952/1‑145 (MQ=255) ccTTCGCCACCTTCCAGCACGCGCTCTTTGATTTCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCAt > 1:585144/1‑149 (MQ=255) cTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGcggcgg < 2:535993/143‑1 (MQ=255) cGCCACCGTCCAGCACGCGCTCTTTGATTGCGTCAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTg < 1:643086/149‑1 (MQ=255) ccACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCCGAACGTCAGAGGAGATACGACCCGTGGGGAAGTTGGTGCGGCGGcacaa > 2:114057/1‑142 (MQ=255) ccACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCAt > 2:490986/1‑143 (MQ=255) cACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCCTTTTGGTa > 1:333978/1‑149 (MQ=255) ttCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTGCGaa > 1:799441/1‑149 (MQ=255) cAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGa < 1:789371/149‑1 (MQ=255) cACGCGCTCTTTGATGGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGaa < 2:339213/143‑1 (MQ=255) gcgcTCTTTGATTTCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCCGCGGCTCATTTTGGTACGAATGa < 2:392658/143‑1 (MQ=255) gcgcTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGa < 2:841423/143‑1 (MQ=255) tctTGGATGGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACg < 2:136482/143‑1 (MQ=255) gATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGt > 2:211525/1‑143 (MQ=255) gATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGcc > 1:603194/1‑149 (MQ=255) aTTGCGTTAGTGATGGGAGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTa < 2:519308/143‑1 (MQ=255) cGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTACTTCACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCccagg > 1:131687/1‑149 (MQ=255) cGTTAGTGATGGCCGGAACAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTGCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGc < 2:12489/143‑1 (MQ=255) gTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCCCGTAGTGcc > 2:720343/1‑143 (MQ=255) gTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAAGGAAAAGATCGtgaggcc > 2:559538/1‑137 (MQ=255) tAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGcccc < 2:646494/143‑1 (MQ=255) tAGTGATGTGCGGAATAACCTGCACGGTCGCGCCGGGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGcccc < 2:337809/143‑1 (MQ=255) gagggATGGGCGGAATAACCTCCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGcccc < 2:820301/139‑1 (MQ=255) tGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTGGCGGCGGCGCATTTTGGTACGAATGAAACGCCCGTAGTGCcccaggcccagg > 1:899206/1‑149 (MQ=255) tgCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGGCCCGTGGTGGAGTTGTTGCGGCGGCTCATTTTGGTGCGAATGAAACGCTCGTCGTGCCCCGGGTCCAGGTCGGt > 1:43237/1‑149 (MQ=255) tgCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGGAGTTGTTGCGGCGGCTCGTTTTGGTACGAATGAAACGGTCGTAGTGCCccaggtccag > 2:648621/1‑143 (MQ=255) gCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGtt < 1:450327/149‑1 (MQ=255) gCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGtt < 1:770839/149‑1 (MQ=255) cGGAATAACCCGCACGGTTGCCCCGAGGTAGTCACCGCGGTGGTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAACGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGttt < 1:335577/149‑1 (MQ=255) ggAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCGCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCGAGGTCGGGTTc > 1:913955/1‑149 (MQ=255) gAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCg < 2:702953/143‑1 (MQ=255) gCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAgcg < 2:738078/143‑1 (MQ=255) tgtgcCCAGGAAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTaa < 1:17242/146‑1 (MQ=255) tacgcCGAGGTAGCCCCCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTaa < 1:232272/147‑1 (MQ=255) gggcCGGGGTAGTCCCCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAAc < 1:224099/147‑1 (MQ=255) cgcCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGATCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCa < 2:597590/143‑1 (MQ=255) gctgAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGAACCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTGCGAGCCGTCTTCAg > 2:23697/4‑143 (MQ=255) gcCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGa > 1:637411/1‑149 (MQ=255) ccGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCTGCGCAGCTCCGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGaa < 1:753511/149‑1 (MQ=255) ccGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCtgccgt > 2:280320/1‑138 (MQ=255) ccGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGaa < 1:371130/149‑1 (MQ=255) ccGAGGGAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGaa < 1:125891/149‑1 (MQ=255) cGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCATAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAac < 1:461514/149‑1 (MQ=255) actccctcggcgTTCTTTACGCAGGACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAaca < 2:716312/136‑1 (MQ=255) tCACCGCGGCGCTCTTTACGCAGAACGTGAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTACTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTcccc > 1:515489/1‑149 (MQ=255) aCCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTTGTGGAGGTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCgt < 1:401809/149‑1 (MQ=255) aCCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCgt < 1:252077/149‑1 (MQ=255) cgcgGCGTTCTTTACGCAGAACGTCAGAGGAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCgtgt < 1:704788/149‑1 (MQ=255) gggcgTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGTTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACCCTTcccc < 2:454849/142‑1 (MQ=255) gcgTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCgt < 2:731279/143‑1 (MQ=255) | CAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGT > CP009273/2902573‑2902858 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |