Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 542,271 IS5 (–) +4 bp 35.0% coding (136‑139/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 269666 =NA (NA)9 (0.510) 8/276 NT 40.2% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 = 542274 13 (0.750)coding (139/1668 nt) fdrA putative NAD(P)‑binding acyl‑CoA synthetase
* ? CP009273 = 270860NA (NA)5 (0.280) 5/276 NT 27.2% noncoding (320/1221 nt) IS30 repeat region
?CP009273 542271 = 13 (0.750)coding (136/1668 nt) fdrA putative NAD(P)‑binding acyl‑CoA synthetase

GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGATGT  >  CP009273/270714‑271007
                                                                                                                                                  |                                                                                                                                                   
gggACAAGAAAATCTCTTTTCTAGTCTGACGGCGATTACTGCTGAATTAACTGTCGGCGAAGGTAACCGGCTGACTCATGATGAACCCTGCTCTATGGCTCCAACTGACACACATGAGCTCATATCAGGGACCTGTCCGCACCTTCCgt                                                                                                                                                   >  1:135688/1‑148 (MQ=11)
    cgaGAACATCGCTTTGCTGGTGTGACGGCGCTTACTGCTGAATCCACGGGCGGCGAAGTGAAAGTGCTGCCAAATGATGAACCCTGTTCTATGGCTCCAGATGACAAACAGTATCTCATATCAGTGACTTGTTCGCACCTTCCGGAGGc                                                                                                                                               <  1:79039/147‑1 (MQ=11)
                          tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                                         >  1:275324/1‑149 (MQ=255)
                                   ttACTGTTGAATTCACTGTCGGCGAAGGGAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGa                                                                                                                <  1:76409/149‑1 (MQ=255)
                                     aCTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACGCTATCTTGTTGAGCTGGCGGCCCTTTTTGTATCTGatt                                                                                                              <  1:67667/149‑1 (MQ=14)
                                       tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattct                                                                                                            >  2:103362/1‑147 (MQ=255)
                                           gAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAAt                                                                                                        <  2:61075/149‑1 (MQ=255)
                                            aaTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATc                                                                                                       >  1:248596/1‑149 (MQ=255)
                                              ttCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATccc                                                                                                     >  2:126614/1‑149 (MQ=255)
                                                cACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCCGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCa                                                                                                   <  2:66299/149‑1 (MQ=255)
                                                cACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCa                                                                                                   <  1:281330/149‑1 (MQ=255)
                                                                                  gaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGt                                                                 >  2:275543/1‑149 (MQ=255)
                                                                                             tATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAggg                                                      <  2:236277/149‑1 (MQ=255)
                                                                                              aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAgggg                                                     >  1:159108/1‑148 (MQ=255)
                                                                                                          gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCAtt                                         >  1:1128/1‑149 (MQ=255)
                                                                                                                     tctcATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACaaa                              >  2:102332/1‑149 (MQ=255)
                                                                                                                                          gCACCTTCCGGAGGCGTTATGAGCTGGGGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTACGAAACGCGGGATAACCAACcttctt         >  1:309335/1‑149 (MQ=255)
                                                                                                                                          gCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTTTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACcttctt         >  1:84100/1‑149 (MQ=255)
                                                                                                                                           cACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCttt        <  2:275324/149‑1 (MQ=255)
                                                                                                                                                 ccGGAGGCGTGATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGATGt  <  1:179638/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTTGGATGT  >  CP009273/270714‑271007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.