Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 62,817 +TCAATT 94.2% intergenic (+82/+229) USA300HOU_0056 → / ← speG hypothetical protein/diamine N‑acetyltransferase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 628172 (0.050)31 (0.880)
+TCAATT
28/244 NT 94.2% intergenic (+82/+229) USA300HOU_0056/speG hypothetical protein/diamine N‑acetyltransferase
?CP000730 62818 = 2 (0.050)intergenic (+83/+228) USA300HOU_0056/speG hypothetical protein/diamine N‑acetyltransferase

TTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTC‑‑‑‑‑‑TATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTAT  >  CP000730/62691‑62949
                                                                                                                              |                                                                                                                                          
ttaAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttg                                                                                                                              <  1:440644‑M1/141‑15 (MQ=255)
  aaaaCAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgtt                                                                                                                            <  2:243138‑M1/141‑17 (MQ=255)
          gCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaatttaa                                                                                                                    >  1:93239‑M1/1‑117 (MQ=255)
            aaaCAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTAGGTTAATTGAAAATAGACAGGAATAAATTGATTGCATCCTTGACCGCGAAGTGGAGTATGCTCtcaatttatttgtgttgaaagtaata                                                                                                                  >  1:109785‑M1/1‑115 (MQ=255)
                  ggCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagct                                                                                                            <  2:60095‑M1/141‑33 (MQ=255)
                  ggCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagct                                                                                                            <  1:238681‑M1/141‑33 (MQ=255)
                     ttACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttca                                                                                                         >  2:261813‑M1/1‑106 (MQ=255)
                     ttACTACAAAAAATGTTTGTGTAATGCTGATAACGTCCTATTATGTTAATTGAAAATAGGCGAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttca                                                                                                         >  1:237601‑M1/1‑106 (MQ=255)
                      tactacAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcat                                                                                                        >  2:79214‑M1/1‑105 (MQ=255)
                               aaaTGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgataga                                                                                               <  1:246860‑M1/141‑46 (MQ=255)
                               aaaTGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgataga                                                                                               >  2:337352‑M1/1‑96 (MQ=255)
                                     ttgaggaattctgaTAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtca                                                                                         <  2:265502‑M1/135‑52 (MQ=255)
                                          tAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaat                                                                                    <  1:420442‑M1/141‑57 (MQ=255)
                                          tAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaat                                                                                    <  2:149025‑M1/141‑57 (MQ=255)
                                          tAATTATTATAACGTCCTATTATTTTAATTAAAAATAGACAAAAATAAATTGAGTGCATCCCTTACTGCAAAGTGAAGGATGCTCTGAATTTATTTTTGTTGAAATTAATTTAAGCTTCATTCTGATAGATATTCATtaat                                                                                    <  2:299166/141‑1 (MQ=255)
                                                         ccTATTATGTTAATCGAAAATAGCCAACAATAAATTGACAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatatatttctgtttaaattcatataagcttaatccttatagttagtcattactaattatttgcaaatc                                                                     >  2:388430‑M1/1‑70 (MQ=255)
                                                                 gTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaa                                                             >  2:360101‑M1/1‑62 (MQ=255)
                                                                       tGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccg                                                       >  1:206297‑M1/1‑56 (MQ=255)
                                                                        gAAAATAGACAAAAAGAAATTGAGAGCACTCCTTTCCTCTAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtatgcaaatcgttattaagtaccgt                                                      <  1:382004‑M1/141‑87 (MQ=255)
                                                                           aaTAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgt                                                   <  2:323339‑M1/141‑90 (MQ=255)
                                                                             tAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgtta                                                 >  2:295542‑M1/1‑50 (MQ=255)
                                                                                  aaaaaTAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcg                                            <  2:245794‑M1/141‑97 (MQ=255)
                                                                                             gagaGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcat                                 <  2:8553‑M1/141‑108 (MQ=255)
                                                                                             gagaGCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcat                                 >  1:58334‑M1/1‑34 (MQ=255)
                                                                                                agCATCCCTTACCGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaa                              <  1:161396‑M1/141‑111 (MQ=255)
                                                                                                         tACCGCAAAGTGAAGGATGCTC‑‑‑‑‑‑TATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGAtttt               <  1:326798/141‑1 (MQ=255)
                                                                                                           ccGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtaga                   >  1:898‑M1/1‑20 (MQ=255)
                                                                                                            tttgcggtaagggatgctctcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagat                  >  1:196131‑M1/12‑19 (MQ=255)
                                                                                                            cGCAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagat                  <  2:347574‑M1/141‑123 (MQ=255)
                                                                                                              cAAAGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagattt                <  1:423583‑M1/141‑125 (MQ=255)
                                                                                                               aaaGTGAAGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagatttt               <  2:61258‑M1/141‑126 (MQ=255)
                                                                                                                     aaGGATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagattttatcaag         <  1:394568‑M1/141‑132 (MQ=255)
                                                                                                                       ggATGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagattttatcaagaa       >  1:387799‑M1/1‑8 (MQ=255)
                                                                                                                         aTGCTCtcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacagcgtcatcattcataaaatcctcgtagattttatcaagaatt     <  1:181528‑M1/141‑136 (MQ=255)
                                                                                                                            ctctcaatttatttttgttgaaattaatataagcttcattctgatagatagtcattaataattgtttgcaaatcgttattaagtaccgttgttacaccgtcatcattcataaaattctcgtagtttttatcaagaatttat  <  2:242658‑M1/141‑139 (MQ=255)
                                                                                                                              |                                                                                                                                          
TTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTC‑‑‑‑‑‑TATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTAT  >  CP000730/62691‑62949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.