Predicted mutation
evidence seq id position mutation freq annotation gene description
JC CP000730 62,817 +TCAATT 94.2% intergenic (+82/+229) USA300HOU_0056 → / ← speG hypothetical protein/diamine N‑acetyltransferase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 628172 (0.050)31 (0.880)
+TCAATT
28/244 NT 94.2% intergenic (+82/+229) USA300HOU_0056/speG hypothetical protein/diamine N‑acetyltransferase
?CP000730 62818 = 2 (0.050)intergenic (+83/+228) USA300HOU_0056/speG hypothetical protein/diamine N‑acetyltransferase

TTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTC‑‑‑‑‑‑TATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTCCTC  >  CP000730/62691‑62954
                                                                                                                                     |                                                                                                                                        
ttaaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTttg                                                                                                                                   <  1:440644‑M2/8‑1 (MQ=255)
  aaaacaacgcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTttgtt                                                                                                                                 <  2:243138‑M2/10‑1 (MQ=255)
          gcaaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAATTTaa                                                                                                                         >  1:93239‑M2/124‑141 (MQ=255)
            aaacaaggcttactacaaaaaatgtttgtgtaattctgataacgtcctattaggttaattgaaaatagacaggaataaattgattgcatccttgaccgcgaagtggagtatgctctcaattTATTTGTGTTGAAAGTAata                                                                                                                       >  1:109785‑M2/122‑141 (MQ=255)
                  ggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCt                                                                                                                 <  1:238681‑M2/26‑1 (MQ=255)
                  ggcttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCt                                                                                                                 <  2:60095‑M2/26‑1 (MQ=255)
                     ttactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCa                                                                                                              >  2:261813‑M2/113‑141 (MQ=255)
                     ttactacaaaaaatgtttgtgtaatgctgataacgtcctattatgttaattgaaaataggcgaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCa                                                                                                              >  1:237601‑M2/113‑141 (MQ=255)
                      tactacaaaaaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCAt                                                                                                             >  2:79214‑M2/112‑141 (MQ=255)
                               aaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTgataga                                                                                                    <  1:246860‑M2/39‑1 (MQ=255)
                               aaatgtttgtgtaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTgataga                                                                                                    >  2:337352‑M2/103‑141 (MQ=255)
                                     ttgaggaattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCa                                                                                              <  2:265502‑M2/45‑1 (MQ=255)
                                          taattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATtaat                                                                                         <  1:420442‑M2/50‑1 (MQ=255)
                                          taattctgataacgtcctattatgttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATtaat                                                                                         <  2:149025‑M2/50‑1 (MQ=255)
                                          tAATTATTATAACGTCCTATTATTTTAATTAAAAATAGACAAAAATAAATTGAGTGCATCCCTTACTGCAAAGTGAAGGATGCTCTGAATTTATTTTTGTTGAAATTAATTTAAGCTTCATTCTGATAGATATTCATtaat                                                                                         <  2:299166/141‑1 (MQ=255)
                                                         cctattatgttaatcgaaaatagccaacaataaattgacagcatcccttaccgcaaagtgaaggatgctctcaataTATTTCTGTTTAAATTCATATAAGCTTAATCCTTATAGTTAGTCATTACTAATTATTTGCAAATc                                                                          >  2:388430‑M2/77‑141 (MQ=255)
                                                                 gttaattgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTaa                                                                  >  2:360101‑M2/69‑141 (MQ=255)
                                                                       tgaaaatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCg                                                            >  1:206297‑M2/63‑141 (MQ=255)
                                                                        gaaaatagacaaaaagaaattgagagcactcctttcctctaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTATGCAAATCGTTATTAAGTACCgt                                                           <  1:382004‑M2/80‑1 (MQ=255)
                                                                           aatagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCgttgt                                                        <  2:323339‑M2/83‑1 (MQ=255)
                                                                             tagacaaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTa                                                      >  2:295542‑M2/57‑141 (MQ=255)
                                                                                  aaaaataaattgagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCg                                                 <  2:245794‑M2/90‑1 (MQ=255)
                                                                                             gagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCAtcattcat                                      <  2:8553‑M2/101‑1 (MQ=255)
                                                                                             gagagcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCAtcattcat                                      >  1:58334‑M2/41‑141 (MQ=255)
                                                                                                agcatcccttaccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATaaa                                   <  1:161396‑M2/104‑1 (MQ=255)
                                                                                                         tACCGCAAAGTGAAGGATGCTC‑‑‑‑‑‑TATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGAtttt                    <  1:326798/141‑1 (MQ=255)
                                                                                                           ccgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGa                        >  1:898‑M2/27‑141 (MQ=255)
                                                                                                            tttgcggtaagggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGAt                       >  1:196131‑M2/26‑141 (MQ=255)
                                                                                                            cgcaaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGAt                       <  2:347574‑M2/116‑1 (MQ=255)
                                                                                                              caaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGAttt                     <  1:423583‑M2/118‑1 (MQ=255)
                                                                                                               aaagtgaaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGAtttt                    <  2:61258‑M2/119‑1 (MQ=255)
                                                                                                                     aaggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAg              <  1:394568‑M2/125‑1 (MQ=255)
                                                                                                                       ggatgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGaa            >  1:387799‑M2/15‑141 (MQ=255)
                                                                                                                         atgctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAAtt          <  1:181528‑M2/129‑1 (MQ=255)
                                                                                                                            ctctcaattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACACCGTCATCATTCATAAAATTCTCGTAGTTTTTATCAAGAATTTAt       <  2:242658‑M2/132‑1 (MQ=255)
                                                                                                                                 aattTATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATACAATCCTCGTAGATTTTATCAAGAATTTATTCctc  <  2:246785‑M2/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
TTAAAACAACGCAAACAAGGCTTACTACAAAAAATGTTTGTGTAATTCTGATAACGTCCTATTATGTTAATTGAAAATAGACAAAAATAAATTGAGAGCATCCCTTACCGCAAAGTGAAGGATGCTC‑‑‑‑‑‑TATTTTTGTTGAAATTAATATAAGCTTCATTCTGATAGATAGTCATTAATAATTGTTTGCAAATCGTTATTAAGTACCGTTGTTACAGCGTCATCATTCATAAAATCCTCGTAGATTTTATCAAGAATTTATTCCTC  >  CP000730/62691‑62954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.