New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 1963216 =61 (0.920)6 (0.090) 5/258 7.8 9.1% intergenic (‑79/+244) USA300HOU_1822/hemG possible hydrolase/protoporphyrinogen oxidase
?CP000730 1963268 = 61 (0.940)intergenic (‑131/+192) USA300HOU_1822/hemG possible hydrolase/protoporphyrinogen oxidase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACTGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGAAAGTATCTA  >  CP000730/1963076‑1963355
                                                                                                                                            |                                                                                                                                           
cACTGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATaaaaa                                                                                                                                             <  1:525944/141‑1 (MQ=255)
  cTGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAaca                                                                                                                                           <  2:720761/141‑1 (MQ=255)
  cTGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAaca                                                                                                                                           >  2:390040/1‑141 (MQ=255)
   tGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAacac                                                                                                                                          >  1:735457/1‑141 (MQ=255)
   tGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAacac                                                                                                                                          >  1:225077/1‑141 (MQ=255)
         cAATTAATTGTACTATTTGCATGCATAGAATGTACAATAAGTACATCTGTCATCTCATTCTCTCCTTTCAAATTACTGCTTCTTTTCTATTATTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTGAt                                                                                                                                    <  2:276006/141‑1 (MQ=255)
              aTTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGaa                                                                                                                               >  1:321309/1‑141 (MQ=255)
                   tactaTTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGtt                                                                                                                          >  1:528329/1‑141 (MQ=255)
                    actaTTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTta                                                                                                                         >  1:540962/1‑141 (MQ=255)
                        tttCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATc                                                                                                                     >  1:550263/1‑141 (MQ=255)
                          tCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGtcactttgc                                                                                                                   >  1:767387/1‑133 (MQ=255)
                            cATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCaa                                                                                                                 >  1:717695/1‑141 (MQ=255)
                              tGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAg                                                                                                               >  1:490116/1‑141 (MQ=255)
                               gCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAAAAAAAAAAAAAAAACACCTTAATTAGAAATGTTATATCGCAAAGt                                                                                                              <  2:548656/141‑1 (MQ=255)
                                      aTGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACAttt                                                                                                       <  1:170203/141‑1 (MQ=255)
                                      aTGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAANTAGAAATGTTATNTCGCAAAGTGACAttt                                                                                                       <  1:741570/141‑1 (MQ=255)
                                       tGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTc                                                                                                      >  1:728401/1‑141 (MQ=255)
                                             ataatTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTa                                                                                                <  1:245430/141‑1 (MQ=255)
                                                atTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAg                                                                                             >  2:256491/1‑141 (MQ=255)
                                                  tACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAgtg                                                                                           >  1:157836/1‑141 (MQ=255)
                                                     aTCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTAt                                                                                        >  1:481482/1‑141 (MQ=255)
                                                      tCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTAtt                                                                                       <  1:62335/141‑1 (MQ=255)
                                                       cTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTAttt                                                                                      >  1:200898/1‑140 (MQ=255)
                                                           cATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATCACTGCTTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATCTCGCAAAGTGACATTTCTAATTAAAGTGTATTGtcat                                                                                  <  1:572071/141‑1 (MQ=255)
                                                              ctcATTCTCTCCTTTCAACTTAATACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAAAAATGTTATATCGCAAAGAGACATTTCTAATTAAAGTGTATTGTcatcat                                                                               >  2:28239/1‑141 (MQ=255)
                                                               tcATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCAtt                                                                              <  2:321309/141‑1 (MQ=255)
                                                                       tcCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATc                                                                      >  2:438647/1‑141 (MQ=255)
                                                                         cTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCAt                                                                    >  2:426819/1‑141 (MQ=255)
                                                                          tttCAACTTACTACTTCTTTTCTATTTTTTTTTTTTTTTTTTTTTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAACTGTATTGTCATCATTTCAattttttt                                                                   >  2:307263/1‑135 (MQ=255)
                                                                          tttCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCAtt                                                                   >  1:365426/1‑141 (MQ=255)
                                                                                cTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGAATACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACAACTCTAATTAAAGTGTATTTTCATCATTTCAATATCATTCaaaaa                                                             >  2:579986/1‑141 (MQ=255)
                                                                                  tactacTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACa                                                           >  1:387558/1‑141 (MQ=255)
                                                                                   actactTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAg                                                          >  2:414734/1‑141 (MQ=255)
                                                                                    ctactTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGc                                                         >  2:454723/1‑141 (MQ=255)
                                                                                            tttCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACAt                                                                                                         >  2:632023/1‑85 (MQ=255)
                                                                                            tttCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACAt                                                                                                         <  1:632023/85‑1 (MQ=255)
                                                                                              tCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTTTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTg                                               >  2:63053/1‑141 (MQ=255)
                                                                                              tCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTg                                               >  1:666342/1‑141 (MQ=255)
                                                                                               cTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGt                                              <  2:464817/141‑1 (MQ=255)
                                                                                                     ttATAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTTATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGc                                        >  2:588214/1‑141 (MQ=255)
                                                                                                     ttAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGc                                        <  1:390040/141‑1 (MQ=255)
                                                                                                          aaaaTGACTGATTACCTATAATTGTAAAATAAAAATACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAACTAAACCTTTGTCTCTGCTTCaa                                   >  2:343600/1‑141 (MQ=255)
                                                                                                          aaaaTGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCaa                                   >  2:304376/1‑141 (MQ=255)
                                                                                                           aaaTGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAAt                                  <  1:138919/141‑1 (MQ=255)
                                                                                                           aaaTGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAAt                                  >  2:300161/1‑141 (MQ=255)
                                                                                                            aaTGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAACGTGTATTGTCATCCTTTCAATATCTTTCAAAAACAGCTAAACCTTTGTCTCTGCTTCCAtt                                 <  2:540962/141‑1 (MQ=255)
                                                                                                            aaTGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAAtt                                 >  1:275987/1‑141 (MQ=255)
                                                                                                                   gATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGAc                                                                                                           >  1:440776/1‑60 (MQ=255)
                                                                                                                   gATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGAc                                                                                                           <  2:440776/60‑1 (MQ=255)
                                                                                                                    aTTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTATAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACaaaa                         >  1:325032/1‑141 (MQ=255)
                                                                                                                      tACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTTAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAaat                       <  1:600198/141‑1 (MQ=255)
                                                                                                                           ataATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTc                  <  1:562252/141‑1 (MQ=255)
                                                                                                                           ataATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTc                  <  1:389295/141‑1 (MQ=255)
                                                                                                                            taATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTcc                 >  2:509483/1‑141 (MQ=255)
                                                                                                                             aaTTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTccc                <  2:387558/141‑1 (MQ=255)
                                                                                                                                 gTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTg            <  1:256491/141‑1 (MQ=255)
                                                                                                                                    aaataaaaACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGaaa         >  1:56052/1‑141 (MQ=255)
                                                                                                                                      ataaaaACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGAAAGt       >  2:768758/1‑141 (MQ=255)
                                                                                                                                         aaaaCACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGAAAGTATc    <  1:768758/141‑1 (MQ=255)
                                                                                                                                          aaaCACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGAAAGTATCt   <  2:56052/141‑1 (MQ=255)
                                                                                                                                           aaCACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGAAAGTATCta  >  1:348494/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CACTGATACCAATGATTTCTACTATTTCCATGCATAGAATGTACAATAATTACATCTGTCATCTCATTCTCTCCTTTCAACTTACTACTTCTTTTCTATTTTTAAAAAAATGACTGATTACCTATAATTGTAAAATAAAAACACCTTAATTAGAAATGTTATATCGCAAAGTGACATTTCTAATTAAAGTGTATTGTCATCATTTCAATATCATTCAAAAACAGCTAAACCTTTGTCTCTGCTTCAATTTCACAAAAATAATTCCCGCTGAAAGTATCTA  >  CP000730/1963076‑1963355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.