mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.26.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 6-Glu_S3_L001_R1_001 | 956,070 | 268,016,423 | 100.0% | 280.3 bases | 301 bases | 99.5% |
| errors | 6-Glu_S3_L001_R2_001 | 956,023 | 268,714,961 | 100.0% | 281.1 bases | 301 bases | 95.9% |
| total | 1,912,093 | 536,731,384 | 100.0% | 280.7 bases | 301 bases | 97.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 116.6 | 4.8 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 2695 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 191 |
| Total length of all junction candidates (factor times the reference genome length) | ≥ 0.1 | 0.025 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.87414 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum coverage each strand | 2 |
| Polymorphism bias cutoff | 0.001 |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥2 bases |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 22:27:33 06 Jul 2015 | 22:28:18 06 Jul 2015 | 45 seconds |
| Read alignment to reference genome | 22:28:18 06 Jul 2015 | 22:32:02 06 Jul 2015 | 3 minutes 44 seconds |
| Preprocessing alignments for candidate junction identification | 22:32:02 06 Jul 2015 | 22:33:00 06 Jul 2015 | 58 seconds |
| Preliminary analysis of coverage distribution | 22:33:00 06 Jul 2015 | 22:35:54 06 Jul 2015 | 2 minutes 54 seconds |
| Identifying junction candidates | 22:35:54 06 Jul 2015 | 22:35:55 06 Jul 2015 | 1 second |
| Re-alignment to junction candidates | 22:35:55 06 Jul 2015 | 22:36:22 06 Jul 2015 | 27 seconds |
| Resolving alignments with junction candidates | 22:36:22 06 Jul 2015 | 22:37:50 06 Jul 2015 | 1 minute 28 seconds |
| Creating BAM files | 22:37:50 06 Jul 2015 | 22:39:32 06 Jul 2015 | 1 minute 42 seconds |
| Tabulating error counts | 22:39:32 06 Jul 2015 | 22:42:22 06 Jul 2015 | 2 minutes 50 seconds |
| Re-calibrating base error rates | 22:42:22 06 Jul 2015 | 22:42:23 06 Jul 2015 | 1 second |
| Examining read alignment evidence | 22:42:23 06 Jul 2015 | 00:29:24 07 Jul 2015 | 1 hour 47 minutes 1 second |
| Polymorphism statistics | 00:29:24 07 Jul 2015 | 00:29:27 07 Jul 2015 | 3 seconds |
| Output | 00:29:27 07 Jul 2015 | 00:30:21 07 Jul 2015 | 54 seconds |
| Total | 2 hours 2 minutes 48 seconds | ||
