Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,606,148	IS2 (+) +5 bp	coding (49‑53/876 nt)	lsrF →	putative autoinducer‑2 (AI‑2) aldolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1467910 =	NA (NA)	90 (0.880)	53/470	0.6	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	1606148 =	0 (0.000)					coding (49/876 nt)	lsrF	putative autoinducer‑2 (AI‑2) aldolase
*	?	NC_000913	= 1469240	NA (NA)	76 (0.740)	47/470	0.8	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 1606152	0 (0.000)					coding (53/876 nt)	lsrF	putative autoinducer‑2 (AI‑2) aldolase

TTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1468994‑1469489                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          ttGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAAcc                                                                                                                                                                                                                                                       <  1:1734/251‑1 (MQ=11)   gCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGAACGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGCCTAGTCTCAAAACTAGTATTAAGACTAACACTTATTTAAGTAATATTGGTTGCCTGGAGATTCAGGGGGCCAGTTTAAAcccc                                                                                                                                                                                                                                                     >  2:695983/1‑251 (MQ=9)      tACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGa                                                                                                                                                                                                                                                  >  1:50161/1‑251 (MQ=32)                 aaTAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATg                                                                                                                                                                                                                                       >  1:858940/1‑251 (MQ=37)                   tAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCt                                                                                                                                                                                                                                     >  1:1094962/1‑251 (MQ=38)                           tggctgCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTACGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCgg                                                                                                                                                                                                                             >  2:1072499/1‑251 (MQ=255)                                   acacCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGaaaa                                                                                                                                                                                                                     <  2:50202/251‑1 (MQ=255)                                      ccATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTg                                                                                                                                                                                                                  >  2:985934/1‑251 (MQ=255)                                       cATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTCTCCTGTGTGGTACGCCGTATCCGTTTCTCCGGCCCTAAGAAATCAATCATCTGTTCCACAATGATTAGTACAAACACTAGTATTAAGACTATTACTTATTTAAGTGATATTGGTTGTCTGGCAATTCAGGGGGCAAGTCTAAACCACAGAACTTACTGAGTCCGATTCCGGCGCGAAAATTGt                                                                                                                                                                                                                 >  2:512507/1‑251 (MQ=14)                                             ggcGGGCAACTAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCGATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTAATTAAGAGATATTGGTTGTCTGGAGATGCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTtaataa                                                                                                                                                                                                           <  2:738183/249‑1 (MQ=255)                                                              aCCGTCATCCCCGGTCCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCa                                                                                                                                                                                          <  1:1072337/251‑1 (MQ=255)                                                               ccGTCATCCCCAGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCaa                                                                                                                                                                                         <  1:893923/251‑1 (MQ=255)                                                                                  gCTCTGCTGCACAATTGGGTTATTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTATGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCCAGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAAATGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTa                                                                                                                                                                      <  2:601684/251‑1 (MQ=255)                                                                                         tgAACGATTGCTATCTTTTCCTGTGTGGTACGCGGTCTGCGTTACTCCTGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGCATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGaaa                                                                                                                                                               <  1:333459/251‑1 (MQ=255)                                                                                         tgAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGaaa                                                                                                                                                               <  1:985772/251‑1 (MQ=255)                                                                                                   cGATCTTTCCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAAcccc                                                                                                                                                                                                                                                     >  2:864426/1‑155 (MQ=14)                                                                                                               gtgtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACCAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGtt                                                                                                                                                                                                                <  2:213179/180‑1 (MQ=255)                                                                                                                tgtgGTACGCCTTCTTCGTTTCTCCGGCCCTAAGACATCCATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGAAGATTCAGGGGGCCTGTCTAATCCCCAGAACTTACTTATGCGGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGtttttt                                                                                                                                        <  1:755648/251‑1 (MQ=255)                                                                                                                     tACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATg                                                                                                                                   <  2:1095129/251‑1 (MQ=255)                                                                                                                               gCGTTTCTCCGG‑CCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTGTTGAATGAGACGAACAtt                                                                                                                        >  1:1058842/1‑251 (MQ=255)                                                                                                                                cGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAACGACTAGTCTAAAAACTAGTATTAAGACTATCACTTAATTAAGTGATATTGGTTGTCTGGGGATTCAAGGTGCCTGTCTAAACCCCAGAACTTACTGATGCTGATTCCAGTGCGAAAATTGTTAATAAAGGTACTTGTAGAGTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACAtt                                                                                                                        <  1:695858/251‑1 (MQ=37)                                                                                                                                       ccGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACCAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAAt                                                                                                                                                                                        >  1:213129/1‑180 (MQ=255)                                                                                                                                        cagCCCTGAGACTTCGATCATCAGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATAATGGTTGTCTTGAGATTCAGAGGGACAGTCTAAACCCCAGAACTTACTTATTCTGAGTCCGGTGCGAAAATTGTGTAAAAAGGTACTTGTTTGTTCAATTGGTCAACGCAAGAGTTATGTGAATACATGTGGTTGCGGATGTTTTTTGACTGACACGAAGATTTAcagcag                                                                                                                <  2:1073569/249‑1 (MQ=21)                                                                                                                                        cGGCCCTAAGACATCAATCATCTGTTCTCCAATTACTAGTCTAAAAACTAGTATTAAGACTATCTCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTTTGCTGATTCCGGTGCGAAAAGTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAGCATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagcag                                                                                                                <  1:663573/251‑1 (MQ=255)                                                                                                                                                  agctccattctcTGTTCTTCAATGGACAGTCTTAAAGCTAGTATTAATACTATCACTTATTTAAGTGATATTTGTTGTCTTGAGGTTCAGGGGGCCAGTCTAAACCCCATAACGTTCTTATGCTGATTCCGGTGCGAATATTGTTAATAAAGGTACTTGTTGTTTCAATTTGTCAACGCAACAGTTATGTGAAAACATGGGATTTCGGAGGTTTTTTGAATGAGACGAACATTTACATCAGAGGAAAAAGc                                                                                                      <  2:668683/241‑1 (MQ=25)                                                                                                                                                                 tctcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACCTCTCAAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCCAAACCCCAGAACTTACTTATGCTGATACCGGTGCGAAAATTTTAAATAAAGGTATATGTAGTTTCAATTGGTCAACGCATCAGTTATGTGAAAACATGGGGTTGCGGAGGTCTACTGAATGAGTCGATCACTTACAGAAGAGGAAAAAGACTCTGTTTTTGAACt                                                                                       >  1:340918/1‑251 (MQ=25)                                                                                                                                                                             aGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTTCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCATAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACgg                                                                           >  1:1050461/1‑251 (MQ=255)                                                                                                                                                                                       cTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTc                                                                 >  1:589784/1‑251 (MQ=255)                                                                                                                                                                                       cTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTc                                                                 >  1:1036119/1‑251 (MQ=255)                                                                                                                                                                                          gTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGtttttt                                                                                                                                        <  1:831375/177‑1 (MQ=255)                                                                                                                                                                                               aaggatATCATTTATTTAAGTGATATTGGTTGTCTGGAGATTCATGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGTTATTTGTAGATTCAATTGGTTAACGCAACAGTTATATGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAAt                                                         <  2:1059004/246‑1 (MQ=255)                                                                                                                                                                                                 gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGAtt                                                                                                                                                                                            <  1:925029/118‑1 (MQ=255)                                                                                                                                                                                                 gACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAg                                                       >  2:683298/1‑251 (MQ=255)                                                                                                                                                                                                      tCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAat                                                  >  1:824860/1‑251 (MQ=255)                                                                                                                                                                                                         cTTATTTCAGAGATATTGGTTGTCTGGGGATTCATGAGGCCAGTCTAAAACCCAGAACTTACTTATTGTGGATCCGGGACGGAAATTGTGAGTAAAGGGACTTGTAGATTCAATTGGTCAACGCAACAGATATGTGAGAACGTGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTa                                                                                      <  2:120259/212‑1 (MQ=18)                                                                                                                                                                                                            aTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTg                                            <  2:124118/251‑1 (MQ=255)                                                                                                                                                                                                            aTTTAAGTGATAATGGTTGTATGGAGATTCAGGGCACCAGTCTAAAACCCAGGAATTACATATGCTGATTGCGGTGCGAAAATTGTTGATAAGGGTACTTGTAGATACAATTGGTCAACGCAACAGTTATATGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGa                                                                                                                             <  1:1027078/170‑1 (MQ=25)                                                                                                                                                                                                              ttAAGTGATATTGGTTGTCTGGAGATTCAGGAGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAAGTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTggg                                          <  2:1036282/251‑1 (MQ=255)                                                                                                                                                                                                                aaGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGtt                                        <  1:683172/251‑1 (MQ=255)                                                                                                                                                                                                                  gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcagcag                                                                                                                >  2:831521/1‑177 (MQ=255)                                                                                                                                                                                                                    gATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCaaa                                    >  1:124075/1‑251 (MQ=255)                                                                                                                                                                                                                    gATATTGGTTGTCTGGAGATTCAGGGCGCCAGTCTACACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAACTGTTAATACAGGTACTTGTATATCCACTTGTTCAACGCAACAGTTTTGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGACACCAACGTTTACAACCGAGGAAAACGCCTCTGTTTTTGACCGATGGAAGAACGGAACAGGCTTAACTCACATCACGAATATCCTAGGTACaca                                    >  2:1062457/1‑249 (MQ=21)                                                                                                                                                                                                                     atatTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGaaa                                                                                                                                                               <  2:705901/127‑1 (MQ=255)                                                                                                                                                                                                                       atTGGTTGTCTTGAGATTCAGGGGGCCAGTCGAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTAATTGTAGATTCAATTAGTCAACGCAACAGTTATGTGAAAACGTGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAcc                                 <  1:37985/251‑1 (MQ=255)                                                                                                                                                                                                                         tGGTTGTCTGGGGATTCGGGGGGCCAGTCTAAACCCCTGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTAAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCg                               <  1:576747/251‑1 (MQ=255)                                                                                                                                                                                                                         tGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTTAACGCAACAGTTAtg                                                                                                                                                                    >  2:925181/1‑118 (MQ=255)                                                                                                                                                                                                                         tGGTTGTATGGAGCTGCAGGGTGCCAGTCTAACCCCCAGAACTTACTTATTCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGAATCAATTGTTTAACGCAACAGTTATGCGAAAACATCGGTTTGCGGATGTTTTTCAAATGAGACAAACATTTACAGAATAGGTAAAAGCCTCTGTCTTTTTAATACAGAAAAACGGAACAGCGATAAGTGAAATATCGAATATCCTGTGCTAaaaaactg                               >  2:762194/1‑247 (MQ=255)                                                                                                                                                                                                                             tGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAAc                           >  1:1011069/1‑251 (MQ=255)                                                                                                                                                                                                                               tCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTAcag                                                                                                                   <  2:413214/161‑1 (MQ=255)                                                                                                                                                                                                                                 tGGAGATTCAGGGGGCTAGTCTAAACCCCAGTACTTACTTATGCTGATTCCGGTGCGAAACTTGTTAATAAAGGTACTTGTAGATTTAATTGGTCAACTCAACAGTTATGTGAAAACATGGGGTAGCGGAGGTTTTTTGAAAGAGACGAACATTTACAGCAGAGGTAAATGCTGCTGTTTGTGAACTAGGGAATAATGGAACAGGCCTTAGTGAAATAGCAAATATCCTGGGTTCAAAAACCGGAACAATc                       >  2:38026/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAAGGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGTTTCAAAACCCGGAACGAta                       >  2:871243/1‑250 (MQ=255)                                                                                                                                                                                                                                   tggtttaaGGGGGCCAGTCTAATCCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTTTTAGTAGAGGTACTTTTAGATTCAATTGGTCAACGGCAAAATTATGTTAGAACATAGGATTGCGTAGGTTTTTTGAATGAGACGAACAGTTACATCCGAAGAAAAAACCTCTGTTTTTGAACTATGGAAGAACTGAACAGGCTTCAGTGAAATAGCGAATATCCTAGGTTCAAAACCCGGAACGATCtt                     <  2:528382/244‑1 (MQ=17)                                                                                                                                                                                                                                          aGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACACGGAACGATCTTCACTATg              >  1:454975/1‑251 (MQ=255)                                                                                                                                                                                                                                           gggggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCATAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCCCTATGt             <  1:512394/251‑1 (MQ=255)                                                                                                                                                                                                                                            ggggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTCGAAGTATGGAAGAACGGAACAGGCTTCAGTGAAAAAGCGAATATCCTGGGCTCaaaatctgtctcttaaaccaatctccg            >  1:546117/1‑228 (MQ=18)                                                                                                                                                                                                                                            ggggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTGGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGATTCAGTGAAATAGAGAATATCCTGGGTTCAAAACACGGAACGATTTTCACTATGtt            >  2:576863/1‑251 (MQ=255)                                                                                                                                                                                                                                             gggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTg                                                                                                                                       >  1:705772/1‑127 (MQ=255)                                                                                                                                                                                                                                             gggCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTa           >  1:386599/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cAGTCTAAACCCCAGGACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAAAATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAggg       <  1:871094/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATgggg                                                                                                                                                       <  2:395916/105‑1 (MQ=255)                                                                                                                                                                                                                                                   gTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCaaa                                    <  1:945643/220‑1 (MQ=255)                                                                                                                                                                                                                                                      tAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTATAGCAGAGGAAAACGCCTATGTTTTTGAACTATGGATGAACGGAACAGGCCTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGTACGATCTTCACTTCGTTAAGGGATACt  >  1:528269/1‑251 (MQ=255)                                                                                                                                                                                                                                                       aaaCCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTg                                                                                           >  1:413123/1‑161 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                          TTGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1468994‑1469489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.